Standardization of substrate and buffer concentrations for in-vivo nitrate reductase activity in Adenanthera microsperma leaves

2015 ◽  
Vol 38 (4) ◽  
pp. 309-311
Author(s):  
Priyanshu Sharma ◽  
S.P. Chaukiyal ◽  
Meenu Sengar
Keyword(s):  
Nitrate Reductase ◽  
Phosphate Buffer ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Buffer Solution ◽  
Substrate Solution ◽  
Substrate Concentrations

The combination of different substrate concentrations (0.05M, 0.10M, 0.15M, 0.20M and 0.25M, KNO3) with different pH of phosphate buffer (0.10 M and 0.20 M, KH2PO4 of the pH 7.0, 7.5, 7.6, 7.7, and 7.8) solutions were tried for in-vivo nitrate reductase activity of Adenanthera microsperma leaves. Maximum nitrate reductase activity was observed in the combination of buffer solution (0.20M KH2PO4) having pH 7.7 and substrate solution 0.20 M concentration.

Standardization of Suitable Substrate and Buffer Solutions for Optimum in-vivo Nitrate Reductase Activity in the Leaves of Sesbania rostrata Bremek. and Oberm.

2016 ◽  
Vol 39 (2) ◽  
pp. 121-124
Author(s):  
S.P. Chaukiyal ◽  
Vandana ◽  
Poornima Uniyal
Keyword(s):  
Nitrate Reductase ◽  
Phosphate Buffer ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Sesbania Rostrata ◽  
Nitrogen Fixing ◽  
Fast Growing ◽  
Buffer Solutions ◽  
Substrate Concentrations

Sesbania rostrata Bremek. and Oberm. is a multipurpose and exceptionally fast growing nitrogen fixing plant. In this study a protocol for the standardization of in-vivo nitrate reductase (NR) assay with respect to substrate concentrations along with buffer pH and concentrations was carried out in the leaves of S. rostrata. Different substrate (KNO3) concentration ( i.e. 0.025M, 0.05M, 0.10M, 0.15M, 0.20M ) were tried against phosphate buffer (KH2PO4) solutions with different pH (6.5, 7.0, 7.5, 7.6, 7.7, 7.8 ). Maximum leaf in-vivo nitrate reductase activity was observed in 0.10 M phosphate buffer (KH2PO4) concentration of pH 7.5 with 0.15M substrate (KNO3) concentration.

SEASONAL PATTERNS OF DENITRIFICATION AND LEAF NITRATE REDUCTASE ACTIVITY IN A CORN FIELD

1978 ◽  
Vol 58 (2) ◽  
pp. 283-285 ◽  
Author(s):  
D. G. PATRIQUIN ◽  
J. C. MacKINNON ◽  
K. I. WILKIE
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
General Trend ◽  
Reductase Activity ◽  
Seasonal Patterns ◽  
Assay Procedure ◽  
Acetylene Blockage Technique ◽  
Acetylene Blockage ◽  
Leaf Nitrate

Denitrification in soil around the bases of corn stalks, determined by the "acetylene blockage technique," exhibited a general trend of decline from June to September. Leaf nitrate reductase activity, determined by an in vivo assay procedure, was low in June and July, and then exhibited a pronounced maximum at the time of tasselling.

Differential effects of ozone on in vivo nitrate reduction in soybean cultivars. I. Response to exogenous sugars

1978 ◽  
Vol 56 (13) ◽  
pp. 1540-1544 ◽  
Author(s):  
Albert C. Purvis
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Foliar Injury ◽  
Glycolytic Intermediates ◽  
Glycine Max L ◽  
Growth Chambers ◽  
Visible Foliar Injury

Two cultivars of soybeans (Glycine max (L.) Merr.) differing widely in their resistance to ozone were exposed to 0.5 μl/ℓ ozone for 2 h in growth chambers. In vivo nitrate reduction was depressed by more than 50% in the primary leaves of Dare, the ozone-sensitive cultivar, but was not significantly altered in Hood, the ozone-resistant cultivar. Sucrose, up to 1.5% (w/v), added to excised seedlings of the Dare cultivar during exposure to ozone eliminated the ozone depression of in vivo nitrate reductase activity and also reduced foliar injury. Addition of two glycolytic intermediates, glyceraldehyde-3-phosphate and fructose-1,6-diphosphate, to the infiltration medium recovered some in vivo nitrate reduction in treated Dare leaves. The levels of extractable nitrate reductase and glyceraldehyde-3-phosphate dehydrogenase in the primary leaves of both cultivars were unaltered by ozone fumigations. These observations led to the conclusion that ozone depression of in vivo nitrate reduction is not due to ozone inactivation of nitrate reductase or of the enzymes coupling nitrate reduction to glycolysis, but may be caused by an inadequate supply of photosynthetic sugars. It was also noted that ozone depression of in vivo nitrate reduction only occurred with treatments which subsequently caused the development of visible foliar injury.

scholarly journals Effects of growth retarding compounds on chlorophyll accumulation and nitrate reductase activity in nitrate induced cucumber cotyledons

2015 ◽  
Vol 42 (3) ◽  
pp. 431-439 ◽  
Author(s):  
J. S. Knypl
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Chlorophyll Synthesis ◽  
Growth Retardants ◽  
Chlorophyll Accumulation ◽  
High Concentrations ◽  
Amo 1618 ◽  
Nr Activity

Cotyledons were excised from 5-day old etiolated cucumber seedlings and .grown for 24 or 48 h in solutions of plant growth retardants: AMO-1618,B-Nine, CCC and phosfon D, supplemented with KNO<sub>3</sub> (10<sup>-2</sup>M) in light. Nitrate reductase (NR) activity was determined <i>in vivo</i>. CCC and Phosfon D at high concentrations had no effect on nitrate reductase activity in 24 h tests. CCC at 5xl0<sup>-2</sup> M enhanced NR activity in longer 48 h tests; Phosfon D was inhibitory in that case. AMO-1618 markedly decreased NR activity. B-Nine strikingly enhanced NR activity in KNO<sub>3</sub> induced cytoledons; the effect was positively correlated with the concentration of B-Nine. Ali the compounds inhibited chlorophyll synthesis.

Changes in Leaf Nitrate Reductase Activity In Vivo and In Vitro During Light-Dark Transitions

1999 ◽  
Vol 8 (1) ◽  
pp. 37-40 ◽  
Author(s):  
B. K. Salalkar ◽  
R. S. Shaikh ◽  
R. M. Naik ◽  
S. V. Munjal ◽  
B. B. Desai ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Leaf Nitrate
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