Five Years’ Evaluation of the BD ProbeTec System for the Direct Molecular Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Clinical Samples

In this study, Mycobacterium tuberculosis complex was detected by BD ProbeTec ET system in 4716 respiratory and 167 nonrespiratory samples [mostly (98%) smear negative]. Sensitivity, specificity, positive and negative predictive values were 81.8%, 98.3, 85.1 and 97.9 for respiratory and 100%, 96.2, 64.7 and 100, for nonrespiratory samples, respectively. Among 149 (3.1%) ProbeTec DTB positive and culture negative samples, 72 (65 respiratory and seven nonrespiratory) (48.3%) were recovered from the patients who were evaluated as having TB infection. The system has been found as useful in early diagnosis of tuberculosis infection in association with the clinical, radiological and histopathological findings.

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Molecular Detection and Identification of Mycobacterium tuberculosis Complex and Four Clinically Important Nontuberculous Mycobacterial Species in Smear-Negative Clinical Samples by the GenoType Mycobacteria Direct Test

Journal of Clinical Microbiology ◽

10.1128/jcm.00612-11 ◽

2011 ◽

Vol 49 (8) ◽

pp. 2874-2878 ◽

Cited By ~ 17

Author(s):

C. Bicmen ◽

A. T. Gunduz ◽

M. Coskun ◽

G. Senol ◽

A. K. Cirak ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Molecular Detection ◽

Mycobacterium Tuberculosis Complex ◽

Clinical Samples ◽

Direct Test ◽

Mycobacterial Species ◽

Tuberculosis Complex ◽

Detection And Identification ◽

Smear Negative

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Evaluation of the Enhanced Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens

Archives of Pathology & Laboratory Medicine ◽

10.5858/1999-123-1101-eoteam ◽

1999 ◽

Vol 123 (11) ◽

pp. 1101-1103 ◽

Cited By ~ 1

Author(s):

Michael B. Smith ◽

John S. Bergmann ◽

Michelle Onoroto ◽

Greg Mathews ◽

Gail L. Woods

Keyword(s):

Mycobacterium Tuberculosis ◽

Nontuberculous Mycobacteria ◽

Direct Detection ◽

Direct Test ◽

Tuberculosis Complex ◽

Predictive Values ◽

Smear Negative ◽

Sensitivity Specificity ◽

Culture Positive ◽

Respiratory Specimens

Abstract Objective.—To evaluate the performance of the enhanced Mycobacterium Tuberculosis Direct Test (E-MTD), for the direct detection of M tuberculosis complex (MTBC) in respiratory specimens. Design.—Two hundred seventy-four respiratory specimens from 151 patients in respiratory isolation were tested with the E-MTD, and the results were compared with the results of mycobacterial smear, culture, and the earlier form of the test, MTD-1. Results.—Forty-one specimens were culture positive for mycobacteria (20 MTBC and 21 nontuberculous mycobacteria), 23 of which were smear positive (16 MTBC, 7 nontuberculous mycobacteria). Twenty-four specimens were positive by E-MTD, and 21 were positive by MTD-1. Of the 20 MTBC culture-positive specimens, 19 were positive by the E-MTD and 19 were positive by the MTD-1. The remaining specimens were MTBC negative by all methods. After resolution of discrepancies, the sensitivity, specificity, and positive and negative predictive values were 95.2%, 100%, 100%, 99.6% for the MTD-1 and 95.2%, 98.8%, 87.0%, and 99.6%, for the E-MTD. For the E-MTD smear-positive and smear-negative specimens, these same values were 93.8%, 100%, 100%, and 87.5% and 100%, 98.8%, 62.5%, and 100%, respectively. Conclusion.—The results suggest that the E-MTD is a reliable method for the direct detection of MTBC in smear-positive respiratory specimens.

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Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex

Eastern Mediterranean Health Journal ◽

10.26719/2004.10.3.329 ◽

2004 ◽

Vol 10 (3) ◽

pp. 329-335

Author(s):

K. K. Abu Amero ◽

M. A. Halablab

Keyword(s):

Mycobacterium Tuberculosis ◽

Mycobacterium Tuberculosis Complex ◽

Tuberculosis Complex ◽

False Negatives ◽

Predictive Values ◽

Lowenstein Jensen ◽

Polymerase Chain ◽

Sensitivity Specificity ◽

Culture Positive ◽

Positive Results

Wevaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samples only. Sensitivity, specificity, and positive and negative predictive values for this test were 92.5%, 100%, 100% and 99.1% respectively. This test is a valuable diagnostic tool for today’s mycobacteriology laboratory

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