Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex

Wevaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samples only. Sensitivity, specificity, and positive and negative predictive values for this test were 92.5%, 100%, 100% and 99.1% respectively. This test is a valuable diagnostic tool for today’s mycobacteriology laboratory

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The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from White rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).

10.21203/rs.2.23897/v2 ◽

2020 ◽

Author(s):

Wynand Johan Goosen ◽

Tanya Jane Kerr ◽

Léanie Kleynhans ◽

Peter Buss ◽

David Cooper ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Mycobacterium Tuberculosis Complex ◽

African Elephants ◽

Tuberculosis Complex ◽

Wildlife Species ◽

Exposed Population ◽

White Rhinoceros ◽

Polymerase Chain ◽

Positive Results ◽

Culture Negative

Abstract Background: Bovine tuberculosis and tuberculosis are chronic infectious diseases caused by the Mycobacterium tuberculosis complex members, Mycobacterium bovis and Mycobacterium tuberculosis , respectively. Infection with M. bovis and M. tuberculosis have significant implications for wildlife species management, public health, veterinary disease control, and conservation endeavours. Results : Here we describe the first use of the VetMAX TM Mycobacterium tuberculosis complex (MTBC) DNA quantitative real-time polymerase chain reaction (qPCR) detection kit for African wildlife samples. DNA was extracted from tissues harvested from 48 African buffaloes and MTBC DNA was detected (test-positive) in all 26 M. bovis culture-confirmed animals with an additional 12 PCR-positive results in culture-negative buffaloes (originating from an exposed population). Of six MTBC-infected African rhinoceros tested, MTBC DNA was detected in antemortem and postmortem samples from five animals. The PCR was also able to detect MTBC DNA in samples from two African elephants confirmed to have M. bovis and M. tuberculosis infections (one each). Culture-confirmed uninfected rhinoceros and elephants’ samples tested negative in the PCR assay.Conclusions: These results suggest this new detection kit is a sensitive screening test for the detection of MTBC-infected African buffaloes, African elephants and white rhinoceros.

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Evaluation of the Enhanced Amplified Mycobacterium Tuberculosis Direct Test for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens

Archives of Pathology & Laboratory Medicine ◽

10.5858/1999-123-1101-eoteam ◽

1999 ◽

Vol 123 (11) ◽

pp. 1101-1103 ◽

Cited By ~ 1

Author(s):

Michael B. Smith ◽

John S. Bergmann ◽

Michelle Onoroto ◽

Greg Mathews ◽

Gail L. Woods

Keyword(s):

Mycobacterium Tuberculosis ◽

Nontuberculous Mycobacteria ◽

Direct Detection ◽

Direct Test ◽

Tuberculosis Complex ◽

Predictive Values ◽

Smear Negative ◽

Sensitivity Specificity ◽

Culture Positive ◽

Respiratory Specimens

Abstract Objective.—To evaluate the performance of the enhanced Mycobacterium Tuberculosis Direct Test (E-MTD), for the direct detection of M tuberculosis complex (MTBC) in respiratory specimens. Design.—Two hundred seventy-four respiratory specimens from 151 patients in respiratory isolation were tested with the E-MTD, and the results were compared with the results of mycobacterial smear, culture, and the earlier form of the test, MTD-1. Results.—Forty-one specimens were culture positive for mycobacteria (20 MTBC and 21 nontuberculous mycobacteria), 23 of which were smear positive (16 MTBC, 7 nontuberculous mycobacteria). Twenty-four specimens were positive by E-MTD, and 21 were positive by MTD-1. Of the 20 MTBC culture-positive specimens, 19 were positive by the E-MTD and 19 were positive by the MTD-1. The remaining specimens were MTBC negative by all methods. After resolution of discrepancies, the sensitivity, specificity, and positive and negative predictive values were 95.2%, 100%, 100%, 99.6% for the MTD-1 and 95.2%, 98.8%, 87.0%, and 99.6%, for the E-MTD. For the E-MTD smear-positive and smear-negative specimens, these same values were 93.8%, 100%, 100%, and 87.5% and 100%, 98.8%, 62.5%, and 100%, respectively. Conclusion.—The results suggest that the E-MTD is a reliable method for the direct detection of MTBC in smear-positive respiratory specimens.

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Five Years’ Evaluation of the BD ProbeTec System for the Direct Molecular Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Clinical Samples

Polish Journal of Microbiology ◽

10.5604/17331331.1185240 ◽

2015 ◽

Vol 64 (4) ◽

pp. 391-394 ◽

Cited By ~ 1

Author(s):

Can Bicmen ◽

Onur Karaman ◽

Ayriz T. Gunduz ◽

Onur F. Erer ◽

Meral Coskun ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Early Diagnosis ◽

Molecular Detection ◽

Mycobacterium Tuberculosis Complex ◽

Clinical Samples ◽

Tuberculosis Complex ◽

Predictive Values ◽

Smear Negative ◽

Sensitivity Specificity ◽

Culture Negative

In this study, Mycobacterium tuberculosis complex was detected by BD ProbeTec ET system in 4716 respiratory and 167 nonrespiratory samples [mostly (98%) smear negative]. Sensitivity, specificity, positive and negative predictive values were 81.8%, 98.3, 85.1 and 97.9 for respiratory and 100%, 96.2, 64.7 and 100, for nonrespiratory samples, respectively. Among 149 (3.1%) ProbeTec DTB positive and culture negative samples, 72 (65 respiratory and seven nonrespiratory) (48.3%) were recovered from the patients who were evaluated as having TB infection. The system has been found as useful in early diagnosis of tuberculosis infection in association with the clinical, radiological and histopathological findings.

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