Molecular Identification of Nocardia Strains from the Soil by hsp65 Gene: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (RFLP) and Analysis of Sequence 16S rRNA Gene

Specific identification of four clam species, Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), Ruditapes philippinarum (Japanese carpet shell), and Venerupis rhomboides (yellow carpet shell), was achieved by polymerase chain reaction–restriction fragment length polymorphism analysis of a fragment of the mitochondrial 16S rRNA gene. Amplification of DNA isolated from the foot muscle produced fragments of 511 bp for V. pullastra, 523 bp for R. decussatus, 545 bp for R. philippinarum, and 502 bp for V. rhomboides. The restriction profiles obtained by agarose gel electrophoresis when amplicons were digested with endonucleases BsmAI and BsrI allowed unequivocal identification of the four clam species. This approach would be less costly, simpler, and quicker than conventional sequencing of polymerase chain reaction products followed by detailed comparison of individual sequences, especially when large numbers of samples need to be analyzed.

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Report of 2 indigenous cases of leprosy from a European country: use of polymerase chain reaction–restriction fragment length polymorphism analysis of hsp65 gene for identification of Mycobacterium leprae directly from a clinical sample

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2009.03.006 ◽

2009 ◽

Vol 64 (3) ◽

pp. 331-333 ◽

Cited By ~ 1

Author(s):

Ioannis K. Neonakis ◽

Zoe Gitti ◽

Fanourios Kontos ◽

Stavroula Baritaki ◽

Loukia Zerva ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

Fragment Length Polymorphism ◽

Clinical Sample ◽

Mycobacterium Leprae ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Hsp65 Gene ◽

Polymerase Chain ◽

Restriction Fragment Length

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The Genetic Relationship Between Mycoplasma-like Organisms Causing Diseases in the Sudan and Different Continents Revealed by Polymerase Chain Reaction (PCR) Amplification of the 16S rRNA Gene Followed by Restriction Fragment Length Polymorphism Analysis

Journal of Phytopathology ◽

10.1111/j.1439-0434.1995.tb00193.x ◽

1995 ◽

Vol 143 (1) ◽

pp. 17-20 ◽

Cited By ~ 2

Author(s):

E. M. Saeed ◽

M. T. Cousin

Keyword(s):

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

Fragment Length Polymorphism ◽

Pcr Amplification ◽

Rrna Gene ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Polymerase Chain ◽

The 16S Rrna Gene ◽

Restriction Fragment Length

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The hsp65 gene patterns of less common Mycobacterium and Nocardia spp. by polymerase chain reaction-restriction fragment length polymorphism analysis with capillary electrophoresis

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2007.02.004 ◽

2007 ◽

Vol 58 (3) ◽

pp. 315-323 ◽

Cited By ~ 12

Author(s):

Po-Ling Chang ◽

Wen-Shyang Hsieh ◽

Chia-Lien Chiang ◽

Marion J. Tuohy ◽

Gerri S. Hall ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Capillary Electrophoresis ◽

Restriction Fragment Length Polymorphism ◽

Length Polymorphism ◽

Fragment Length Polymorphism ◽

Polymorphism Analysis ◽

Chain Reaction ◽

Hsp65 Gene ◽

Polymerase Chain ◽

Restriction Fragment Length

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Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2003000800003 ◽

2003 ◽

Vol 38 (8) ◽

pp. 911-920 ◽

Cited By ~ 10

Author(s):

Marcelo Ferreira Fernandes ◽

Roberta Pereira Miranda Fernandes ◽

Mariangela Hungria

Keyword(s):

Polymerase Chain Reaction ◽

Restriction Fragment Length Polymorphism ◽

16S Rrna ◽

Cajanus Cajan ◽

Fragment Length Polymorphism ◽

23S Rrna ◽

Chain Reaction ◽

Gene 16S Rrna ◽

Polymerase Chain ◽

Restriction Fragment Length

O objetivo deste trabalho foi caracterizar geneticamente sete estirpes de rizóbios nativos dos tabuleiros costeiros de Sergipe com alta eficiência de fixação biológica do N2 em associação com guandu (Cajanus cajan) e caupi (Vigna unguiculata). A amplificação do DNA pela técnica de PCR (polymerase chain reaction) com o oligonucleotídeo específico BOX indicou um grau elevado de diversidade genética, uma vez que todas as estirpes apresentaram perfis únicos de DNA. A análise por BOX-PCR revelou, ainda, que essa metodologia é eficiente para diferenciar estirpes, mas não para a diferenciação de espécies de rizóbio. Pela técnica do RFLP (restriction fragment length polymorphism) da região do DNA que codifica o gene 16S rRNA e da região intergênica entre os genes 16S e 23S rRNA, com cinco enzimas de restrição, bem como pelo seqüenciamento parcial da região do 16S rRNA, foi possível classificar as estirpes nos gêneros Bradyrhizobium e Rhizobium. Houve coerência entre as análises envolvendo a região do 16S rRNA, mas o agrupamento com uma das estirpes diferiu pela análise do espaço intergênico. Os resultados obtidos com a estirpe R11 indicam variabilidade genética elevada em relação às espécies de rizóbios descritas, inclusive diferindo em diversas bases da região do 16S rRNA, e podem indicar uma nova espécie.

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