Anthelmintic Activity of Nine Varieties of Cajanus cajan (L.) Millsp on Haemonchus contortus from Sheep

Background: Internal parasitosis is the most important parasitism in small ruminants in tropical region. Synthetic anthelmintics are usually used for their control. Due to the emergence of resistance in worm population, the use of alternative methods such as plants bioactive molecules are developed. This study aimed at assessing anthelmintic activity of nine varieties of Cajanus cajan, a taniferous plant cultivated in West Africa. Methods: Leaves of the nine varieties of Cajanus cajan were dried, ground and hydroacetonic extracts were obtained by cold maceration at a concentration of 60 mg/ml. Live adult of Haemonchus contortus were obtained from sheep’s stomach and exposed, in triplicate, to the following solutions: hydroacetonic extracts (60 mg/ml), hydroacetonic extracts (60 mg/ml) associated with polyvinylpyrrolidone (PVP) (50 mg/ml), Levamisole (20 mg/ml) and phosphate buffered saline (PBS) solution. Worm death time was recorded for each treatment. Result: Worms exposed to Levamisole recorded the shortest death time about 9.73±1.77 min. The potent of the extracts induced worm death time of 64.83±4.73 min while the least efficient induced a death time of 156.50±23.20 min. Worms in the PBS solution were still alive after 24 hours of exposure. These results indicated that the four varieties of C. cajan used in this study have promising wormicidal. Besides that, the effect of tannins were not the only compound responsible for the anthelmintic activity.

In silico profiling of histone deacetylase inhibitory activity of compounds isolated from Cajanus cajan

Background Cancer is responsible for high morbidity and mortality globally. Because the overexpression of histone deacetylases (HDACs) is one of the molecular mechanisms associated with the development and progression of some diseases such as cancer, studies are now considering inhibition of HDAC as a strategy for the treatment of cancer. In this study, a receptor-based in silico screening was exploited to identify potential HDAC inhibitors among the compounds isolated from Cajanus cajan, since reports have earlier confirmed the antiproliferative properties of compounds isolated from this plant. Results Cajanus cajan-derived phytochemicals were docked with selected HDACs, with givinostat as the reference HDAC inhibitor, using AutodockVina and Discovery Studio Visualizer, BIOVIA, 2020. Furthermore, absorption, distribution, metabolism and excretion (ADME) drug-likeness analysis was done using the Swiss online ADME web tool. From the results obtained, 4 compounds; betulinic acid, genistin, orientin and vitexin, were identified as potential inhibitors of the selected HDACs, while only 3 compounds (betulinic acid, genistin and vitexin) passed the filter of drug-likeness. The molecular dynamic result revealed the best level of flexibility on HDAC1 and HDAC3 compared to the wild-type HDACs and moderate flexibility of HDAC7 and HDAC8. Conclusions The results of molecular docking, pharmacokinetics and molecular dynamics revealed that betulinic acid might be a suitable HDAC inhibitor worthy of further investigation in order to be used for regulating conditions associated with overexpression of HDACs. This knowledge can be used to guide experimental investigation on Cajanus cajan-derived compounds as potential HDAC inhibitors.

DEVELOPMENT AND CHARACTERIZATION OF ORO-DISPERSIBLE TABLETS OF METFORMIN HYDROCHLORIDE USING CAJANUS CAJAN STARCH AS A NATURAL SUPERDISINTEGRANT

Objective: The aim of the research work was to explore the use of Cajanus cajan (Pigeon pea) polysaccharide as a superdisintegrant. The novel superdisintegrant has been evaluated for its action by incorporating it into orodispersible tablets of Metformin Hydrochloride. Methods: Cajanus cajan starch was extracted from its seeds and superdisintegrant was developed by microwave modification of the extract. Various characterization tests such as gelatinization temperature, water absorption index, pH, and viscosity were used to identify the microwave-modified polysaccharide. The orodispersible tablets were made using a direct compression process employing varying concentrations of modified Cajanus cajan starch. Prepared tablets were tested for several pre and post-compression parameters and compared with a well-established synthetic superdisintegrant, sodium starch glycolate. The stability studies were conducted on an optimized formulation. Results: Fourier transform infrared spectroscopy study showed that the drug had no interactions with the microwave-modified Cajanus cajan starch. SEM confirmed that Cajanus cajan starch granules exhibited intact granular structure in oval shapes and smooth surfaces. After microwave modification, the Cajanus cajan starch component lost its granular structure, which further led to the generation of surface pores and internal channels, causing overall swelling responsible for superdisintegrant activity. The optimized formulation (ODF5) containing 15 % modified Cajanus cajan starch performed better in terms of wetting time (22.21 s), disintegration time (53.3 s), and in vitro drug release (92%), as compared to formulation prepared by synthetic superdisintegrant (ODF1). Conclusion: The present investigation concluded that modified Cajanus cajan starch has good potential as a superdisintegrant for formulating oro-dispersible tablets. Furthermore, modified Cajanus cajan starch is inexpensive, non-toxic and compatible in comparison with available synthetic superdisintegrants.

Performance of Pigeon Pea (Cajanus cajan) Varieties under Different Soil Amendments in Ndokwa, Delta State, Nigeria

Background: Pigeon pea is an important legume worldwide that is not cultivated by the farmers in Ndokwa, Delta State. Hence, the study looked at the possibility of evaluating the potential of pigeon pea in Ogume, a community in Ndokwa. Methods: Top soils were collected from a fallowed land mixed thoroughly and filled into 4 kg bags and were divided into 3. The 1st was amended with poultry manure, 2nd was NPK 15:15:15 amended and 3rd was without amendment, tested with two varieties of Cajanus cajan. The experiment was a 2 by 3 factorial, in a completely randomized design with 3 replicates. Residual effects of the amendments were evaluated in 2020. Soil samples were taken to establish the fertility status before amendment and at harvest. Data were taken on the agronomic and yield parameters and were analyzed using ANOVA while means were separated using LSD at P≤0.05. Result: Result obtained showed that the tallest plants (9.83±0.51) were observed in the poultry manure in 2019 while the fallowed plots had the tallest plants (11.17±0.49) in 2020, while in both years, the number of pods and dry pod weight was insignificant among the treatments and varieties. This shows that farmers do not need to spend much finance in amending the soil for pigeon pea cultivation in Ndokwa community, Delta State.

Quantification of soil temperature in sole and intercropped pigeonpea (Cajanus cajan L. Millsp.) using meteorological parameters

The meteorological week-wise soil thermal regime in the root zone (5, 15 and 30 cm depth) of pigeonpea and pigeonpea based groundnut (Arachis hypogaea) cropping systems was studied in relation to various meteorological parameters twice a day, 0738 and 1438 hrs (IST) for three years (1986-87, 1987-88 and 1988-89), in the middle Gujarat region. A decline in soil thermal regime was observed on three occasions during the crop growth period viz., at onset of SW monsoon, at the end of rainy season and at the time of harvesting of intercrop, In sole pigeonpea, the soil temperature upto 30 cm depth can be estimated from mean air temperature, whereas in the pigeonpea + groundnut cropping system, before harvest of intercrop the minimum and maximum temperature were found to be more appropriate for estimation of morning and afternoon time respectively, but only in the top layers of the soil.