scholarly journals In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

2016 ◽  
Vol 15 (24) ◽  
pp. 1269-1275 ◽  
Author(s):  
Abu Hena Mostofa Jamal Mohammad ◽  
Hoque Sharif Imdadul ◽  
Mostofa Shakil Md ◽  
N M Rubaiyath Bin Rahman A ◽  
Akhter Banu Nilufa ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Ocimum Sanctum ◽  
Mass Propagation

scholarly journals Seaweed Extract Promotes High-Frequency in vitro Regeneration of Solanum surattense Burm.f:A Valuable Medicinal Plant

2017 ◽  
Vol 11 (4) ◽  
pp. 134-141
Author(s):  
Packiaraj Gurusarava ◽  
Sadasivam Vinoth ◽  
Ganesan Prem Kumar ◽  
Pandiselvi .
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Seaweed Extract ◽  
Solanum Surattense

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

In vitro Regeneration and Conservation of Rare Medicinal Plant Dregea volubilis Benth.

2011 ◽  
Vol 6 (6) ◽  
pp. 225-228 ◽  
Author(s):  
N. Yogananth ◽  
R. Bhakyaraj ◽  
A. Chanthuru ◽  
S. Palanivel
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

scholarly journals Histological studies of in vitro regeneration induced in leaf explants of Nymphoides cristatum (Roxb) O.Kuntze – An aquatic medicinal plant

1970 ◽  
Vol 3 ◽  
Author(s):  
Mallapa Hanumanthu Niranjan ◽  
Mysore Shankar Sudarshana
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Free Medium ◽  
Histological Studies ◽  
Leaf Histology ◽  
Benzyl Amino Purine

The objective of this work was to study the histological events related to the regeneration process of a medicinal plant, Nymphoides cristatum (Roxb). Leaf explants were cultured on MS medium supplemented with 0.5 mgl-1 of 6-benzyl amino purine (BAP). About 90% of explants gave rise to shoots after 15 days of incubation. The histological studies showed that the regeneration originated directly from parenchymatous cells and direct organogenesis after 20 days of culture could be observed. Buds and roots were found completely differentiated after 40 days of culture and number of shoots per explants was 30. Micorshoots were rooted in hormone - free medium and the plants obtained grew in artificial pond under green house conditions. Key words: Leaf, Histology, in vitro regeneration, Nymphoides cristatum.  DOI: 10.3126/ijls.v3i0.2370

scholarly journals A Novel Method for Efficient Micropropagation of Radermachera xylocarpa (Roxb.) K. Schum., a Rare Medicinally Important Forest Species

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
Manjary Sathe ◽  
Megha Vibhute ◽  
Monica Jain ◽  
Pankaj Srivastav
Keyword(s):  
Tree Species ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Forest Tree ◽  
Root Induction ◽  
Mass Propagation ◽  
Forest Tree Species ◽  
Bud Induction ◽  
Indigenous Forest

Radermachera xylocarpa (Roxb.) K. Schum. is a rare indigenous forest tree species which is utilized for its wood and medicinal properties. Due to its overexploitation and specific habitat requirements the species is restricted to limited areas. In vitro mass propagation of tree species faces various challenges and no such efforts have yet been taken in propagation of this useful plant using these methods.  In order to overcome the hurdles and understanding an urgent need of its conservation and mass propagation present authors attempt to develop a simple effective tissue culture protocol for regeneration of R. xylocarpa. Nodal explants were cultured on MS supplemented with various concentrations of cytokinins and auxins.  Among different cytokinins, maximum bud induction and proliferation was obtained in media supplemented with Kn along with IBA and for effective root induction which is tough to obtain in tree species, 100% rooting was achieved in cultures with increasing concentrations of IBA. Field survival is a major challenge with regenerated plants of forest tree species. We report here for the first time 100% survival of plants in soil by carefully standardizing the period of hardening and acclimatization procedures. A novel and effective in vitro regeneration protocol of R. xylocarpa has been successfully standardized which can be adopted for large scale propagation, reforestation and conservation of rare Radermachera xylocarpa of medicinal importance.Plant Tissue Cult. & Biotech. 23(1): 21?29, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15556

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