In vitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone

2022 ◽  
Author(s):  
Preeti Choudhary ◽  
Vinod Kataria
Keyword(s):  
Medicinal Plant ◽  
In Vitro Culture ◽  
Arid Zone ◽  
Mass Propagation ◽  
Sarcostemma Acidum

Lead Induced Responses of Pfaffia glomerata, an Economically Important Brazilian Medicinal Plant, Under In Vitro Culture Conditions

2011 ◽  
Vol 86 (3) ◽  
pp. 272-277 ◽  
Author(s):  
D. K. Gupta ◽  
F. T. Nicoloso ◽  
M. R. Schetinger ◽  
L. V. Rossato ◽  
H. G. Huang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Culture ◽  
Culture Conditions ◽  
Induced Responses ◽  
Pfaffia Glomerata

scholarly journals Results with the establishment of in vitro culture of Leucojum aestivum

2007 ◽  
Vol 13 (2) ◽  
Author(s):  
E. Kohut ◽  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Culture ◽  
In Vitro Cultures ◽  
Naphthalene Acetic Acid ◽  
Benzyl Adenine ◽  
Leucojum Aestivum ◽  
Leaves And Roots ◽  
Bulbous Plant

Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too.

scholarly journals In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

2016 ◽  
Vol 15 (24) ◽  
pp. 1269-1275 ◽  
Author(s):  
Abu Hena Mostofa Jamal Mohammad ◽  
Hoque Sharif Imdadul ◽  
Mostofa Shakil Md ◽  
N M Rubaiyath Bin Rahman A ◽  
Akhter Banu Nilufa ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Ocimum Sanctum ◽  
Mass Propagation

scholarly journals Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

2017 ◽  
Vol 5 (2) ◽  
pp. 15-26 ◽  
Author(s):  
Raihan I Raju ◽  
Shyamal K Roy
Keyword(s):  
In Vitro Culture ◽  
Basal Medium ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Mass Propagation ◽  
Surface Sterilization ◽  
Half Strength

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog’s (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.Jahangirnagar University J. Biol. Sci. 5(2): 15-26, 2016 (December)

scholarly journals In vitro Mass Propagation from Shoot Tip Explants of Vernonia cinerea (L.) Less. An Antioxidant, Anti-inflammatory Medicinal Plant

1970 ◽  
Vol 20 (2) ◽  
pp. 127-131 ◽  
Author(s):  
M. Maharajan ◽  
Abdul Bakrudeen Ali Ahmed ◽  
Rosna Mat Taha ◽  
S. Jawahar ◽  
R. Ravi Paul ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Key Words ◽  
Plant Tissue ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Anti Inflammatory ◽  
Shoot Tip Explants ◽  
Vernonia Cinerea ◽  
Number Of Shoots

Plantlets were regenerated from the shoot tip explants of Vernonia cinerea (L.) Less. in MS supplimented with BA and Kn. Maximum number of shoots in BA (13.32 mg/l) and roots in IBA (7.38 mg/l) developed. The rooted plantlets were successfully established in the field.   Key words: Vernonia cinerea, Mass propagation, Shoot tip explants, Antioxidant, Medicinal plant   D.O.I. 10.3329/ptcb.v20i2.6892   Plant Tissue Cult. & Biotech. 20(2): 127-131, 2010 (December)

scholarly journals In vitro regeneration for mass propagation in commercial scale of medicinal plant vitex nigundo L.

1970 ◽  
Vol 18 ◽  
pp. 140-145 ◽  
Author(s):  
Md Abu Hena Mostofa Jamal ◽  
ANM Rubaiyath-Bin Rahman ◽  
Dipak Kumar Paul ◽  
Md Rezuanul Islam
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Commercial Scale

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferation and root induction were recorded after three weeks of inoculation and used for calculation. For each treatment 15 explants were used and all the treatments were repeated thrice. Established plantlets were transplanted in earthen pots under natural conditions and the survival rate was recorded. Results: The most effective surface sterilization treatment has been found 0.1 % HgCl2 for 7 minutes. Highest number of shoot was observed in MS medium supplemented with 3.0 mg/ BAP. It was rooted well in full MS containing 2.0 mg/l IAA. The survival rate was 85 % and propagated plantlets were successfully acclimatized in soil. Conclusion: It was observed that shoot tips are more responsive for micropropagation of Vitex nigundo L . Thus the fruitful utilization of rapid clonal propagation, germplasm conservation and distribution of Vitex nigundo, important medicinal plant of Bangladesh, is possible. Keywords: Vitex nigundo; Medicinal plant; Shoot induction; Micropropagation; Regeneration. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8790 JBS 2010; 18(0): 140-145

In vitro mass propagation and conservation of a rare medicinal plant, Zhumeria Majdae Rech.f & Wendelbo (Lamiaceae)

2019 ◽  
Vol 17 ◽  
pp. 318-325 ◽  
Author(s):  
Maryam Fallah ◽  
Mohsen Farzaneh ◽  
Morteza Yousefzadi ◽  
Mansour Ghorbanpour ◽  
Mohammad Hossein Mirjalili
Keyword(s):  
Medicinal Plant ◽  
Mass Propagation
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