Molecular marker-based validation of blast resistance gene Pi54 and identification of potential donors in temperate high altitude rice (Oryza sativa L.)

In the course of marker-assessted selection for the blast resistance gene Pi-b, among 372 rice Oryza sativa L. doubled haploids DH0 geterozygous plant was identified. In the offspring (11 doubled haploids DH1), only Pi-b resistance allele was revealed. The reason for the appearance of false heterozygosity is discussed. By analogy with mixoploidy, an explanation is proposed for the phenomenon of somatic tissues mixogeny.

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Role of the rice blast resistance gene Pi‐cd in rice ( Oryza sativa ) breeding programmes

Plant Breeding ◽

10.1111/pbr.12842 ◽

2020 ◽

Vol 139 (5) ◽

pp. 845-852

Author(s):

Kenji Fujino ◽

Mari Obara ◽

Hitoshi Kiuchi ◽

Hiroshi Shinada ◽

Tsutomu Nishimura ◽

...

Keyword(s):

Oryza Sativa ◽

Resistance Gene ◽

Rice Blast ◽

Blast Resistance ◽

Rice Blast Resistance ◽

Blast Resistance Gene ◽

Breeding Programmes

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Identification of rapd marker linked to blast resistance gene in a somaclone of rice cultivar Araguaia

Fitopatologia Brasileira ◽

10.1590/s0100-41582002000200010 ◽

2002 ◽

Vol 27 (2) ◽

pp. 181-185 ◽

Cited By ~ 2

Author(s):

LEILA G. ARAÚJO ◽

ANNE S. PRABHU ◽

MARTA C. FILIPPI

Keyword(s):

Oryza Sativa ◽

Resistance Gene ◽

Bulked Segregant Analysis ◽

Blast Resistance ◽

Rapd Marker ◽

Rice Cultivar ◽

Pyricularia Grisea ◽

Blast Resistance Gene ◽

F2 Population ◽

Susceptible Bulk

The gene Pi-ar confers resistance to Pyricularia grisea race IB-45 in a somaclone derived from immature panicles of the susceptible rice (Oryza sativa) cultivar Araguaia. RAPD technique was used to identify molecular markers linked to this gene utilizing bulked segregant analysis. Initially, the two parental DNAs from the resistant donor SC09 and 'Araguaia' were analyzed using random primers. Of the 240 primers tested, 203 produced amplification products. The two parental DNAs along with the resistant and susceptible bulks of F2 population were screened using 48 primers that differentiated resistant and susceptible parents. Even though eight primers differentiated the resistant bulk from the susceptible bulk, as well as somaclone SC09 and 'Araguaia', only one primer, OPC02 ('GTGAGGCGTC'), was found to be tightly linked (1.7cM) to the resistance gene of somaclone SC09.

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Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice

Genome ◽

10.1139/g96-004 ◽

1996 ◽

Vol 39 (1) ◽

pp. 26-30 ◽

Cited By ~ 40

Author(s):

Naweed I. Naqvi ◽

Bharat B. Chattoo

Keyword(s):

Resistance Gene ◽

Rapd Markers ◽

Oryza Sativa L ◽

Blast Resistance ◽

Major Gene ◽

Indirect Selection ◽

Sequence Information ◽

Scar Markers ◽

Blast Resistance Gene ◽

Selection Of

Two randomly amplified polymorphic DNA (RAPD) markers, OPF62700 and OPH182400, tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international race IB46) of the blast fungus were identified. To derive sequence characterized amplified regions (SCARs) from OPF62700 and OPH182400, these amplified RAPD products were cloned and sequenced. Nucleotide sequence information, obtained for each end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCAR's alternate alleles were considered for the indirect selection of Pi-10. Such polymorphisms converted the linked dominant RAPD loci into codominant SCAR markers and also facilitated the indirect scoring of the blast-resistant and blast-susceptible genotypes. The development of length variant codominant SCAR markers linked to a major gene for blast resistance in rice is described. The codominant SCARs will facilitate marker-assisted selection of the Pi-10 locus in rice breeding programs and will also be useful as genetic markers for high resolution mapping of the Pi-10 region. Key words : SCAR, Pyricularia grisea, Oryza sativa L., blast resistance, indirect selection.

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