HETEROZYGOUS ORYZA SATIVA L. DOUBLED HAPLOID GENERATED THROUGH IN VITRO ANDROGENESIS

2021 ◽  
Author(s):  
M.V. Ilyushko ◽  
◽  
M.V. Romashova
Keyword(s):  
Oryza Sativa ◽  
Doubled Haploid ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Doubled Haploids ◽  
Resistance Allele ◽  
Blast Resistance Gene ◽  
Somatic Tissues ◽  
Selection For

In the course of marker-assessted selection for the blast resistance gene Pi-b, among 372 rice Oryza sativa L. doubled haploids DH0 geterozygous plant was identified. In the offspring (11 doubled haploids DH1), only Pi-b resistance allele was revealed. The reason for the appearance of false heterozygosity is discussed. By analogy with mixoploidy, an explanation is proposed for the phenomenon of somatic tissues mixogeny.

scholarly journals Cyto-histological aspects of haploid androgenesis when obtaining haploids/doubled haploids in rice (Oryza Sativa l.) anther culture in vitro

2021 ◽  
Vol 285 ◽  
pp. 02033
Author(s):  
E. G. Savenko ◽  
Zh. M. Mukhina ◽  
V. A. Glazyrina ◽  
L. A. Shundrina
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Histological Study ◽  
Doubled Haploids ◽  
Abnormal Development ◽  
Characteristic Functions ◽  
Stage Of Development

The aim of the study was to study the development of rice microspores in anther culture in vitro, study the structure of androclinic callus to identify cyto-embryological features of the formation of morphogenic structures in anther culture, obtain doubled rice haploids (Oryza sativa L.) and accelerate the development of valuable breeding material with desired properties. Within the framework of this study, the results of a cyto-histological study of rice haploid androgenesis in vitro were obtained, which indicate that it is induced under the influence of phytohormones from rice anther microspores at the mononuclear or early binuclear stage of development. The abnormal development of microspores on nutrient media with phytohormone 2,4-D was traced, in which nuclei, having lost their characteristic functions, acquired the ability to unlimited division and growth with the formation of microcallus. The morphological structure of calli was assessed. The main morphotypes of callus tissues and the pathways of morphogenesis, leading to the formation of androgenic structures, up to full-fledged regenerant plants, were identified. Homozygous androgenic lines based on F1 and BC1 - rice generations obtained in the course of hybridization and backcrossing between Chinese samples carrying blast resistance genes and Russian accessiond were rapidly developed.

scholarly journals SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.)

2011 ◽  
Vol 30 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Sadegh Ashkani ◽  
Mohd Yusop Rafii ◽  
Ibrahim Rusli ◽  
Meon Sariah ◽  
Siti Nor Akmar Abdullah ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Marker Assisted Selection ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Selection For

scholarly journals Characterization of Pi13, a blast resistance gene that maps to chromosome 6 in indica rice (Oryza sativa L. variety, Kasalath)

2011 ◽  
Vol 61 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Takeshi Ebitani ◽  
Nagao Hayashi ◽  
Motoyasu Omoteno ◽  
Hidenobu Ozaki ◽  
Masahiro Yano ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Indica Rice ◽  
Chromosome 6 ◽  
Blast Resistance Gene

scholarly journals Use of DNA Markers Closely Linked to a Blast Resistance Gene, Piz, in Rice (Oryza sativa L.) Breeding

2005 ◽  
Vol 7 (3) ◽  
pp. 143-146
Author(s):  
Noriaki Hashimoto ◽  
Masaaki Daigen ◽  
Keiko Hayashi ◽  
Ikuo Ashikawa ◽  
Yoshinori Takahara ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Dna Markers ◽  
Oryza Sativa L ◽  
Blast Resistance ◽  
Blast Resistance Gene
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