scholarly journals The Effects of BunSimGiEumGami-Bang(Fenxinqiyinjiameifang) on Serotonin of P815 cell

2011 ◽  
Vol 22 (2) ◽  
pp. 147-162 ◽  
Author(s):  
Jae-Won Lim ◽  
In-Chul Jung ◽  
Sang-Ryong Lee
Keyword(s):  
P815 Cell

Reactive Oxygen Species-Mediated Apoptosis and Cytotoxicity of Newly Synthesized Pyridazin-3-Ones In P815 (Murin Mastocytoma) Cell Line

Drug Research ◽  
2019 ◽  
Vol 69 (10) ◽  
pp. 528-536
Author(s):  
Najat Bouchmaa ◽  
Reda Ben Mrid ◽  
Youness Boukharsa ◽  
Youssef Bouargalne ◽  
Mohamed Nhiri ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Redox Homeostasis ◽  
Reactive Oxygen ◽  
Cytotoxic Activities ◽  
Oxygen Species ◽  
P815 Cell

Abstract Background In cancer cells, the intracellular antioxidant capacity and the redox homeostasis are mainly maintained by the glutathione- and thioredoxin-dependent systems which are considered as promising targets for anticancer drugs. Pyridazinones constitute an interesting source of heterocyclic compounds for drug discovery. The present investigation focused on studying the in-vitro antitumor activity of newly synthesized Pyridazin-3(2h)-ones derivatives against P815 (Murin mastocytoma) cell line. Methods The in-vitro cytotoxic activities were investigated toward the P815 cell line using tetrazolium-based MTT assay. Lipid peroxidation and the specific activities of antioxidant enzymes were also determined. Results The newly compounds had a selective dose-dependent cytotoxic effect without affecting normal cells (PBMCs). Apoptosis was further confirmed through the characteristic apoptotic morphological changes and DNA fragmentation. Two compounds (6f and 7h) were highly cytotoxic and were submitted to extend biological testing to determine the likely mechanisms of their cytotoxicity. Results showed that these molecules may induce cytotoxicity via disturbing the redox homeostasis. Importantly, the anticancer activity of 6f and 7h could be due to the intracellular reactive oxygen species hypergeneration through significant loss of glutathione reductase and thioredoxin reductase activities. This eventually leads to oxidative stress-mediated P815 cell apoptosis. Furthermore, the co-administration of 6f or 7h with Methotrexate exhibited a synergistic cytotoxic effect. Conclusions considering their significant anticancer activity and chemosensitivity, 6f and 7h may improve the therapeutic efficacy of the current treatment for cancer.

scholarly journals Evaluation of the anaphylactoid potential of Andrographis paniculata extracts using the popliteal lymph node assay and P815 cell degranulation in vitro

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Xuguang Hu ◽  
Ya Wen ◽  
Shasha Liu ◽  
Jiabo Luo ◽  
Xiaomei Tan ◽  
...  
Keyword(s):  
Lymph Node ◽  
Andrographis Paniculata ◽  
Popliteal Lymph Node ◽  
P815 Cell ◽  
Popliteal Lymph Node Assay

scholarly journals The Effects of OnDam-tang-Kami-bang (ODK) in Antioxidant and Serotonin Metabolism Testing on P815 Cell

2013 ◽  
Vol 24 (2) ◽  
pp. 189-200 ◽  
Author(s):  
Seon-Hui Seol ◽  
Sang Ryong Lee ◽  
In Chul Jung
Keyword(s):  
Serotonin Metabolism ◽  
P815 Cell

scholarly journals Nitric oxide inhibits irreversibly P815 cell proliferation: involvement of potassium channels

2002 ◽  
Vol 35 (6) ◽  
pp. 321-332 ◽  
Author(s):  
R. S. A. Costa ◽  
J. Assreuy
Keyword(s):  
Nitric Oxide ◽  
Cell Proliferation ◽  
Potassium Channels ◽  
P815 Cell
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