Peer Review #2 of "Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and geographical variations in shell form (v0.1)"

Leptopomais a species rich genus with approximately 100 species documented. Species-level identification in this group has been based on shell morphology and colouration, as well as some anatomical features based on small sample sizes. However, the implications of the inter- and intra-species variations in shell form to the taxonomy ofLeptopomaspecies and the congruency of its current shell based taxonomy with its molecular phylogeny are still unclear. There are fourLeptopomaspecies found in Sabah, Borneo, and their taxonomy status remains uncertain due to substantial variation in shell forms. This study focuses on the phylogenetic relationships and geographical variation in shell form of threeLeptopomaspecies from Sabah. The phylogenetic relationship of these species was first estimated by performing Maximum Likelihood and Bayesian analysis based on mitochondrial genes (16S rDNA and COI) and nuclear gene (ITS-1). Then, a total of six quantitative shell characters (i.e., shell height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height to width) and three qualitative shell characters (i.e., shell colour patterns, spiral ridges, and dark apertural band) of the specimens were mapped across the phylogenetic tree and tested for phylogenetic signals. Data on shell characters ofLeptopoma sericatumandLeptopoma pellucidumfrom two different locations (i.e., Balambangan Island and Kinabatangan) where both species occurred sympatrically were then obtained to examine the geographical variations in shell form. The molecular phylogenetic analyses suggested that each of the threeLeptopomaspecies was monophyletic and indicated congruence with only one of the shell characters (i.e., shell spiral ridges) in the current morphological-based classification. Although the geographical variation analyses suggested some of the shell characters indicating inter-species differences between the twoLeptopomaspecies, these also pointed to intra-species differences between populations from different locations. This study onLeptopomaspecies is based on small sample size and the findings appear only applicable toLeptopomaspecies in Sabah. Nevertheless, we anticipate this study to be a starting point for more detailed investigations to include the other still little-known (ca. 100)Leptopomaspecies and highlights a need to assess variations in shell characters before they could be used in species classification.

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Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and variations in shell form due to geography

10.7287/peerj.preprints.3078v1 ◽

2017 ◽

Author(s):

Chee-Chean Phung ◽

Pooi-San Heng ◽

Thor-Seng Liew

Keyword(s):

Molecular Phylogeny ◽

Phylogenetic Relationship ◽

Species Differences ◽

Shell Height ◽

Nuclear Gene ◽

Land Snails ◽

Shell Form ◽

Geographical Variations ◽

Colour Patterns ◽

Relationship Of

Leptopoma is a species rich genus with approximately 100 species documented according to shell morphology and animal anatomy. Many of the Leptopoma species are described in terms of shell size, shape, sculpture and colour patterns of a small number of examined materials. However, the implications of the inter- and intra-species variations in shell form to the taxonomy of Leptopoma species and the congruency of its current shell based taxonomy with its molecular phylogeny are still unclear. Over the last decade, more than 900 collection lots consisting of more than 4000 Leptopoma specimens have been obtained in Sabah and deposited in BORNEENSIS at Universiti Malaysia Sabah. Access to this collection gave us the opportunity to examine the geographical variations in shell forms and the phylogenetic relationship of Leptopoma species in Sabah. The phylogenetic relationship of three Leptopoma species was first estimated by performing maximum likelihood and Bayesian analysis based on mitochondrial genes (16S and COI) and nuclear gene (ITS-1). After this, a total of six quantitative shell characters (i.e. shell height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height and width) and three qualitative shell characters (i.e. shell colour patterns, spiral ridges, and dark ring band in aperture) of the specimens were mapped across the phylogenetic tree and tested for phylogenetic signals. Data on shell characters of Leptopoma sericatum and Leptopoma pellucidum from two different locations (i.e. Balambangan Island and Kinabatangan) where both species occurred sympatrically were then obtained to examine the geographical variations in shell form. The molecular phylogenetic analyses suggested that each of the three Leptopoma species was monophyletic and indicated congruence with one of the shell characters (i.e. shell spiral ridges) in the current morphological-based classification. Other qualitative and quantitative shell characters were incongruent with the Leptopoma species phylogeny. Although the geographical variation analyses suggested some of the shell characters indicating inter-species differences between the two Leptopoma species, these also pointed to intra-species differences between populations from different locations. This study provides an initiation to resolve the taxonomy conundrum for the remaining 100 little known Leptopoma species from other regions and highlights a need to assess variations in shell characters before they could be used in species classification.

Download Full-text

Land snails of Leptopoma Pfeiffer, 1847 in Sabah, Northern Borneo (Caenogastropoda: Cyclophoridae): an analysis of molecular phylogeny and variations in shell form due to geography

10.7287/peerj.preprints.3078 ◽

2017 ◽

Author(s):

Chee-Chean Phung ◽

Pooi-San Heng ◽

Thor-Seng Liew

Keyword(s):

Molecular Phylogeny ◽

Phylogenetic Relationship ◽

Species Differences ◽

Shell Height ◽

Nuclear Gene ◽

Land Snails ◽

Shell Form ◽

Geographical Variations ◽

Colour Patterns ◽

Relationship Of

Leptopoma is a species rich genus with approximately 100 species documented according to shell morphology and animal anatomy. Many of the Leptopoma species are described in terms of shell size, shape, sculpture and colour patterns of a small number of examined materials. However, the implications of the inter- and intra-species variations in shell form to the taxonomy of Leptopoma species and the congruency of its current shell based taxonomy with its molecular phylogeny are still unclear. Over the last decade, more than 900 collection lots consisting of more than 4000 Leptopoma specimens have been obtained in Sabah and deposited in BORNEENSIS at Universiti Malaysia Sabah. Access to this collection gave us the opportunity to examine the geographical variations in shell forms and the phylogenetic relationship of Leptopoma species in Sabah. The phylogenetic relationship of three Leptopoma species was first estimated by performing maximum likelihood and Bayesian analysis based on mitochondrial genes (16S and COI) and nuclear gene (ITS-1). After this, a total of six quantitative shell characters (i.e. shell height, shell width, aperture height, aperture width, shell spire height, and ratio of shell height and width) and three qualitative shell characters (i.e. shell colour patterns, spiral ridges, and dark ring band in aperture) of the specimens were mapped across the phylogenetic tree and tested for phylogenetic signals. Data on shell characters of Leptopoma sericatum and Leptopoma pellucidum from two different locations (i.e. Balambangan Island and Kinabatangan) where both species occurred sympatrically were then obtained to examine the geographical variations in shell form. The molecular phylogenetic analyses suggested that each of the three Leptopoma species was monophyletic and indicated congruence with one of the shell characters (i.e. shell spiral ridges) in the current morphological-based classification. Other qualitative and quantitative shell characters were incongruent with the Leptopoma species phylogeny. Although the geographical variation analyses suggested some of the shell characters indicating inter-species differences between the two Leptopoma species, these also pointed to intra-species differences between populations from different locations. This study provides an initiation to resolve the taxonomy conundrum for the remaining 100 little known Leptopoma species from other regions and highlights a need to assess variations in shell characters before they could be used in species classification.

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