scholarly journals Connectivity map of bipolar cells and photoreceptors in the mouse retina

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Christian Behrens ◽  
Timm Schubert ◽  
Silke Haverkamp ◽  
Thomas Euler ◽  
Philipp Berens
Keyword(s):  
Electron Microscopy ◽  
Plexiform Layer ◽  
Bipolar Cells ◽  
Mouse Retina ◽  
Connectivity Map ◽  
Dendritic Field ◽  
Electron Microscopy Data ◽  
Different Types ◽  
Microscopy Data ◽  
Anatomical Evidence

In the mouse retina, three different types of photoreceptors provide input to 14 bipolar cell (BC) types. Classically, most BC types are thought to contact all cones within their dendritic field; ON-BCs would contact cones exclusively via so-called invaginating synapses, while OFF-BCs would form basal synapses. By mining publically available electron microscopy data, we discovered interesting violations of these rules of outer retinal connectivity: ON-BC type X contacted only ~20% of the cones in its dendritic field and made mostly atypical non-invaginating contacts. Types 5T, 5O and 8 also contacted fewer cones than expected. In addition, we found that rod BCs received input from cones, providing anatomical evidence that rod and cone pathways are interconnected in both directions. This suggests that the organization of the outer plexiform layer is more complex than classically thought.

scholarly journals Connectivity map of bipolar cells and photoreceptors in the mouse retina

2016 ◽  
Author(s):  
Christian Behrens ◽  
Timm Schubert ◽  
Silke Haverkamp ◽  
Thomas Euler ◽  
Philipp Berens
Keyword(s):  
Visual Processing ◽  
Plexiform Layer ◽  
Bipolar Cells ◽  
Mouse Retina ◽  
Connectivity Map ◽  
Dendritic Field ◽  
Electron Microscopy Data ◽  
Different Types ◽  
Microscopy Data ◽  
Anatomical Evidence

AbstractVisual processing begins at the first synapse of the visual system. In the mouse retina, three different types of photoreceptors provide input to 14 bipolar cell (BC) types. Classically, most BC types are thought to contact all cones within their dendritic field; ON BCs would contact cones exclusively via so-called invaginating synapses, while OFF BCs would form basal synapses. By mining publically available electron microscopy data, we discovered interesting violations of these rules of outer retinal connectivity: ON BC type X contacted only ~20% of the cones in its dendritic field and made mostly atypical non-invaginating contacts. Types 5T, 5O and 8 also contacted fewer cones than expected. In addition, we found that rod BCs received input from cones, providing anatomical evidence that rod and cone pathways are interconnected in both directions. This suggests that the organization of the outer plexiform layer is more complex than classically thought.

The Development of the Outer Plexiform Layer of the Mouse Retina

1972 ◽  
Vol 30 ◽  
pp. 56-57
Author(s):  
Janet C. Blanks ◽  
Anthony M. Adinolfi ◽  
Richard N. Lolley
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Adult Mouse ◽  
Light And Electron Microscopy ◽  
Plexiform Layer ◽  
Uranyl Acetate ◽  
Bipolar Cells ◽  
Outer Plexiform Layer ◽  
Mouse Retina ◽  
Lead Citrate

In the all-rod retina of the adult mouse, synaptic contacts in the outer plexiform layer occur between photoreceptor terminals and dendritic processes of horizontal and bipolar cells. The postnatal development of the outer plexiform layer has been studied by light and electron microscopy. Following decapitation and enucleation of the eyes, samples of retinae from DBA mice (newborn to fourteen days) were removed and fixed by immersion in solutions of buffered glutaraldehyde and paraformaldehyde. The tissue was postfixed in osmium tetroxide, dehydrated and embedded in araldite. Sections were stained with uranyl acetate and lead citrate.With light microscopy, the outer plexiform layer first appears in the central retina as a gap within the neuroblastic layer on the fifth postnatal day. The separation is completed by the seventh postnatal day and appears mature by the fourteenth postnatal day.

scholarly journals Label-free fluorescence predictions from large-scale correlative light and electron microscopy data

2021 ◽  
Vol 27 (S1) ◽  
pp. 94-95
Author(s):  
Ryan Lane ◽  
Luuk Balkenende ◽  
Simon van Staalduine ◽  
Anouk Wolters ◽  
Ben Giepmans ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Light And Electron Microscopy ◽  
Label Free ◽  
Electron Microscopy Data ◽  
Microscopy Data

scholarly journals Automatic instance segmentation of mitochondria in electron microscopy data

2021 ◽  
Author(s):  
Luke Nightingale ◽  
Joost de Folter ◽  
Helen Spiers ◽  
Amy Strange ◽  
Lucy M Collinson ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Large Scale ◽  
Machine Learning Algorithms ◽  
Public Repository ◽  
Post Processing ◽  
Human Cortex ◽  
Electron Microscopy Data ◽  
Processing Procedure ◽  
Microscopy Data ◽  
Instance Segmentation

We present a new method for rapid, automated, large-scale 3D mitochondria instance segmentation, developed in response to the ISBI 2021 MitoEM Challenge. In brief, we trained separate machine learning algorithms to predict (1) mitochondria areas and (2) mitochondria boundaries in image volumes acquired from both rat and human cortex with multi-beam scanning electron microscopy. The predictions from these algorithms were combined in a multi-step post-processing procedure, that resulted in high semantic and instance segmentation performance. All code is provided via a public repository.

scholarly journals Влияние температуры деформации в камере Бриджмена на особенности формирования дефектной структуры технически чистого титана

2018 ◽  
Vol 44 (20) ◽  
pp. 70
Author(s):  
Н.А. Шурыгина ◽  
А.М. Глезер ◽  
Д.Л. Дьяконов ◽  
А.А. Томчук ◽  
А.Г. Кадомцев ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Defect Structures ◽  
Cryogenic Temperatures ◽  
Two Phase ◽  
Α Phase ◽  
Electron Microscopy Data ◽  
Phase Mixture ◽  
Microscopy Data

AbstractTransmission electron microscopy data showed evidence of the formation of structural regions corresponding to deformation (dislocated) fragments and dynamically recrystallized grains in α-phase titanium upon torsion at high hydrostatic pressure at room and cryogenic temperatures. It is shown that the previously proposed “two-phase mixture” model is applicable to description of these defect structures.

Sign in / Sign up

Export Citation Format

Share Document