scholarly journals A Python script to merge Sanger sequences

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11354
Author(s):  
Cen Chen ◽  
Bingguo Lu ◽  
Xiaofang Huang ◽  
Chuyun Bi ◽  
Lili Zhao ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Python Script

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.

scholarly journals A Python script to merge Sanger sequences

2020 ◽  
Author(s):  
Cen Chen ◽  
Xiaofang Huang ◽  
Bingguo Lu ◽  
Chuyun Bi ◽  
Lili Zhao ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Python Script

Abstract Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to merge multiple overlapping Sanger sequences. Our results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.

Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shaan 253

2010 ◽  
Vol 36 (3) ◽  
pp. 526-532
Author(s):  
Ming-Xia WANG ◽  
Xiang GAO ◽  
Qi-Jiao CHEN ◽  
Jian DONG ◽  
Wan-Chun ZHAO ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Gene Cloning ◽  
Wheat Cultivar

Gene cloning, tissue expression and preparation of monoclonal antibody of Lateolabrax japonicus PPARγ

2011 ◽  
Vol 34 (8) ◽  
pp. 1156-1164
Author(s):  
Yun-xia QIAN ◽  
Sun-xiao YANG ◽  
Hong LIANG ◽  
Lun QIAN ◽  
Kai-xian QIAN
Keyword(s):  
Monoclonal Antibody ◽  
Gene Cloning ◽  
Tissue Expression ◽  
Lateolabrax Japonicus

scholarly journals Recipes for recombining DNA: A history of Molecular Cloning: A Laboratory Manual

BJHS Themes ◽  
2020 ◽  
Vol 5 ◽  
pp. 225-243
Author(s):  
Angela N.H. Creager
Keyword(s):  
Gene Cloning ◽  
Molecular Cloning ◽  
Recombinant Dna ◽  
Laboratory Manual ◽  
The Bible ◽  
Rapid Spread ◽  
History Of ◽  
Laboratory Manuals ◽  
Late Twentieth ◽  
Cold Spring

AbstractLaboratory instructions and recipes are sometimes edited into books with a wide circulation. Even in the late twentieth century, publications of this nature remained influential. For example, protocols from a 1980 summer course on gene cloning at Cold Spring Harbor Laboratory provided the basis for a bestselling laboratory manual by Tom Maniatis, Ed Fritsch and Joe Sambrook. Not only did the Molecular Cloning: A Laboratory Manual become a standard reference for molecular biologists (commonly called the ‘bible’), but also its recipes and clear instructions made gene cloning and recombinant DNA technologies accessible to non-specialists. Consequently, this laboratory manual contributed to the rapid spread of genetic-engineering techniques throughout the life sciences, as well as in industry. As is often the case with how-to books, however, finding a way to update methods in this rapidly changing field posed a challenge, and various molecular-biology reference books had different ways of dealing with knowledge obsolescence. This paper explores the origins of this manual, its publication history, its reception and its rivals – as well as the more recent migration of such laboratory manuals to the Internet.

scholarly journals A novel prokaryotic phospholipase A2. Characterization, gene cloning, and solution structure.

2002 ◽  
Vol 277 (32) ◽  
pp. 29348
Author(s):  
Masanori Sugiyama ◽  
Kazuhiro Ohtani ◽  
Miho Izuhara ◽  
Tohru Koike ◽  
Koji Suzuki ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Gene Cloning ◽  
Solution Structure
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