Gene cloning and analysis of the pattern of expression of the transcription factor HymMYB2 related to blue flower formation in Hydrangea macrophylla

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Jiqing Peng ◽  
Chao Xue ◽  
Xujie Dong ◽  
Chaozhen Zeng ◽  
Yi Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cloning ◽  
Blue Flower ◽  
Flower Formation ◽  
Hydrangea Macrophylla

Palynology of uppermost Proterozoic and lowermost Cambrian formations, central Mackenzie Mountains, northwestern Canada

1989 ◽  
Vol 26 (1) ◽  
pp. 129-148 ◽  
Author(s):  
D. Baudet ◽  
J. D. Aitken ◽  
M. Vanguestaine
Keyword(s):  
Rocky Mountains ◽  
Lower Cambrian ◽  
Russian Platform ◽  
Blue Flower ◽  
Flower Formation ◽  
Early Cambrian ◽  
Canadian Rocky Mountains ◽  
Structural Units ◽  
Upper Proterozoic ◽  
Lithostratigraphic Correlation

The acritarch assemblages of strata from the base of the Upper Proterozoic Sheepbed Formation to the base of the Lower Cambrian (Atdabanian) Sekwi Formation are described. The sections sampled are in southwestern (internal) structural units where erosion beneath the "sub-Cambrian"(?) unconformity is least evident. Problems of lithostratigraphic correlation of post-Sheepbed, pre-Backbone Ranges formations remain. Acritarchs indicate the age of the Sheepbed Formation and the Blue-flower Formation above it is latest Proterozoic (Vendian), whereas that of the Vampire Formation is Early Cambrian (Atdabanian). The Backbone Ranges Formation has not yielded datable acritarchs, but it is for the most part Cambrian in age, based on other fossil evidence. Comparisons are made with the Russian Platform and southern Canadian Rocky Mountains successions. The total number of acritarch genera increases markedly across the Precambrian–Cambrian transition.

scholarly journals Nonsense Mutation of an MYB Transcription Factor Is Associated with Purple-Blue Flower Color in Soybean

2011 ◽  
Vol 102 (4) ◽  
pp. 458-463 ◽  
Author(s):  
R. Takahashi ◽  
E. R. Benitez ◽  
M. E. Oyoo ◽  
N. A. Khan ◽  
S. Komatsu
Keyword(s):  
Transcription Factor ◽  
Nonsense Mutation ◽  
Flower Color ◽  
Myb Transcription Factor ◽  
Blue Flower

scholarly journals Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Qian Wu ◽  
Jie Wu ◽  
Shan-Shan Li ◽  
Hui-Jin Zhang ◽  
Cheng-Yong Feng ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Blue Flower ◽  
Flower Formation ◽  
Metabolite Analysis

scholarly journals 643 The Effect of Nitrogen and Sulfur Applications on Hydrangeas

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 508C-508
Author(s):  
Willie Helpingstine ◽  
Ellen T. Paparozzi ◽  
Walter W. Stroup
Keyword(s):  
Flower Color ◽  
Blue Flower ◽  
Leaf Color ◽  
Design Data ◽  
Color Chart ◽  
Hydrangea Macrophylla ◽  
Royal Horticultural Society ◽  
Flower Diameter ◽  
Four Levels ◽  
Treatment Design

Hydrangeas are sold as a potted florist plant during the spring, usually around Mothers Day and Easter. They are considered “heavy feeders” because of their high requirement for nitrogen. Two experiments were conducted to determine if the addition of sulfur (S) would allow lower rates of nitrogen (N) to be applied without sacrificing plant color and quality. Hydrangea macrophylla `Blue Danube' were fertilized with four levels of N (50, 100, 200, and 450 ppm) in combination with six levels of S (0, 6, 12, 24, 48, and 96 ppm) during a typical forcing program. The experimental design was a randomized complete block with a complete factorial treatment design. Data collected included visual observations (using the Royal Horticultural Society Color Chart) on leaf color and uniformity of flower color as well as flower shape. Quantitative data included flower diameter, floret diameter, height, and N an S leaf concentrations. Soil pH was monitored throoughout the experiment and remained fairly constant (range of 5.0–6.0). Additional sulfur seemed to have no effect on leaf color at the higher levels of N. Lower concentrations of N produced more true blue flower color. Also, at lower N concentrations, higher S resulted in larger flowers with larger florets.

scholarly journals The Use of Recycled Waste Paper as an Al Source for Blue-flowering Hydrangea

HortScience ◽  
1998 ◽  
Vol 33 (4) ◽  
pp. 604b-604
Author(s):  
K.M. Ryan ◽  
J.H. Edwards ◽  
C.H. Gilliam ◽  
G.J. Keever
Keyword(s):  
Waste Paper ◽  
Aluminum Concentration ◽  
Blue Flower ◽  
Blue Color ◽  
Time Control ◽  
Color Development ◽  
Hydrangea Macrophylla ◽  
The Media ◽  
Recycled Waste ◽  
Recycled Waste Paper

Blue color development in Hydrangea macrophylla is usually accomplished by applying Al as an alum drench. Drenches are applied during forcing 10–14 days after transplanting at a rate of 17,500 mg·L-1. Cultivars Blue Wave and Nikko Blue were used to evaluate if the Al contained in waste paper can provide the necessary Al for blue flower development. Two waste paper forms, pelletized and crumble, were used as surface mulches and as media amendments. The amendments were incorporated into the media at transplanting and mulches were applied either at transplanting or 28 days later. Alum drenching was initiated at transplanting as a control. Leachates were collected weekly using the VTEM. Total Al, electrical conductivity, and pH were determined on all samples. All waste paper treatments resulted in pink flowers in both cultivars. Leachate pH, from plants in this test, was >6.5. Aluminum concentration was greater than the 15 mg·L-1 Al needed for blue color development in flowers, but Al concentration decreased with time. Control of pH at the waste paper surface and in the media is critical for increasing the availability of labile Al for uptake by hydrangea.

scholarly journals Exploring the Molecular Mechanism of Blue Flower Color Formation in Hydrangea macrophylla cv. “Forever Summer”

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiqing Peng ◽  
Xujie Dong ◽  
Chao Xue ◽  
Zhiming Liu ◽  
Fuxiang Cao
Keyword(s):  
Transcription Factors ◽  
Molecular Mechanism ◽  
Color Change ◽  
Anthocyanin Biosynthesis ◽  
Flower Color ◽  
Anthocyanin Synthesis ◽  
Blue Flower ◽  
Biosynthesis Pathway ◽  
Color Formation ◽  
Hydrangea Macrophylla

Hydrangea macrophylla has a large inflorescence and rich colors, which has made it one of the most popular ornamental flowers worldwide. Thus far, the molecular mechanism of flower color formation in H. macrophylla flowers is unknown. By comparing the pigment content and transcriptome data of the bud period (FSF1), discoloration period (FSF2) and full-bloom stage (FSF3) of infertile blue flowers of H. macrophylla cv. “Forever Summer,” we found that genes associated with anthocyanin production were most associated with the formation of blue infertile flowers throughout development. The anthocyanin biosynthesis pathway is the main metabolic pathway associated with flower color formation, and the carotenoid biosynthesis pathway appeared to have almost no contribution to flower color. There was no competition between the flavonoid and flavonol and anthocyanin biosynthesis pathways for their substrate. At FSF1, the key genes CHS and CHI in the flavonoid biosynthesis pathway were up-regulated, underlying the accumulation of a substrate for anthocyanin synthesis. By FSF3, the downstream genes F3H, C3′5′H, CYP75B1, DFR, and ANS in the anthocyanin biosynthesis pathway were almost all up-regulated, likely promoting the synthesis and accumulation of anthocyanins and inducing the color change of infertile flowers. By analyzing protein–protein interaction networks and co-expression of transcription factors as well as differentially expressed structural genes related to anthocyanin synthesis, we identified negatively regulated transcription factors such as WER-like, MYB114, and WDR68. Their site of action may be the key gene DFR in the anthocyanin biosynthesis pathway. The potential regulatory mechanism of flower color formation may be that WER-like, MYB114, and WDR68 inhibit or promote the synthesis of anthocyanins by negatively regulating the expression of DFR. These results provide an important basis for studying the infertile flower color formation mechanism in H. macrophylla and the development of new cultivars with other colors.

Gene cloning of ZmMYB59 transcription factor in maize and its expression during seed germination in response to deep-sowing and exogenous hormones

2017 ◽  
Vol 136 (6) ◽  
pp. 834-844 ◽  
Author(s):  
Longgang Du ◽  
Hongye Jiang ◽  
Guangwu Zhao ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Gene Cloning ◽  
Exogenous Hormones

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression
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