scholarly journals Molecular analysis of oral microflora in patients with primary Sjögren’s syndrome by using high-throughput sequencing

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5649 ◽  
Author(s):  
Zhifang Zhou ◽  
Guanghui Ling ◽  
Ning Ding ◽  
Zhe Xun ◽  
Ce Zhu ◽  
...  
Keyword(s):  
Dental Caries ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
Primary Sjogren’S Syndrome ◽  
Core Microbiome ◽  
Primary Sjögren's Syndrome ◽  
The Core ◽  
Oral Microflora

BackgroundThe objective of this study was to characterize the oral microflora profile of primary Sjögren’s syndrome (pSS) patients, thereby revealing the connection between oral bacterial composition and dental caries, and to identify the “core microbiome” in the oral cavities of pSS patients and systemic healthy individuals by using a high-throughput sequencing technique.MethodsTwenty-two pSS patients and 23 healthy controls were enrolled in this study. Their clinical data and oral rinse samples were collected. The V3–V4 hypervariable regions of the bacterial 16S rRNA gene of samples were amplified and analyzed by high-throughput sequencing on the Illumina Miseq PE300 platform.ResultsBoth two groups were age- and sex-matched. There were significantly higher decayed, missing and filled teeth (DMFT) and decayed, missing and filled surfaces (DMFS) in the pSS group than in the control group (p < 0.01). Alpha diversity was depleted in pSS patients, compared with healthy controls (p < 0.01), while beta diversity between the two groups was not significantly different. Seven discriminative genera (LDA > 4) were found between the two groups in LEfSe (LDA Effect Size) analysis. The relative abundance ofVeillonellain pSS patients was fourfold higher, whileActinomyces,Haemophilus,Neisseria,Rothia,PorphyromonasandPeptostreptococcuswere significantly lower in pSS patients than in healthy controls. However, the correlation betweenVeillonellaand DMFT/DMFS was not significant (p > 0.05). In Venn diagram analysis, nine genera shared by all samples of two groups, which comprised 71.88% and 67.64% in pSS patients and controls, respectively.DiscussionThese findings indicate a microbial dysbiosis in pSS patients; notably,Veillonellamight be recognized as a biomarker in pSS patients. The core microbiome in pSS patients was similar to the systemic healthy population. These provide insight regarding advanced microbial prevention and treatment of severe dental caries in pSS patients. This study also provides basic data regarding microbiology in pSS.

scholarly journals Chemosensory Dysfunction, Oral Disorders and Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome: Comparative Cross-Sectional Study

2020 ◽  
Author(s):  
Mirjana Šijan Gobeljić ◽  
Vera Milić ◽  
Nada Pejnovic ◽  
Nemanja Damjanov
Keyword(s):  
Oral Health ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
Primary Sjogren’S Syndrome ◽  
Age And Gender ◽  
Primary Sjögren's Syndrome ◽  
And Gender ◽  
Sjögren‘S Syndrome

Abstract Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls. Methods: Olfactory and gustatory function, dysgeusia, burning sensations in the tongue (BST), halitosis, and oral health-related quality of life (OHRQoL) using the short-form Oral Health Impact Profile (OHIP-14) were evaluated in 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls in this comparative cross-sectional study. Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%) as evaluated using Sniffin Sticks test and taste stripts, respectively. A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001) while similar number of pSS patients and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared to controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison to the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.

scholarly journals Chemosensory Dysfunction and Oral Disorders Affect Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome

2020 ◽  
Author(s):  
Mirjana Šijan Gobeljić ◽  
Vera Milić ◽  
Nada Pejnovic ◽  
Nemanja Damjanov
Keyword(s):  
Oral Health ◽  
Sjogren’S Syndrome ◽  
Sjogren's Syndrome ◽  
Primary Sjögren’S Syndrome ◽  
Healthy Controls ◽  
Primary Sjogren’S Syndrome ◽  
Age And Gender ◽  
Primary Sjögren's Syndrome ◽  
And Gender ◽  
Sjögren‘S Syndrome

Abstract Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls.Methods: Olfactory and gustatory function, dysgeusia, burning sensations in the tongue (BST), halitosis, and oral health-related quality of life (OHRQoL) using the short-form Oral Health Impact Profile (OHIP-14) were evaluated in 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls.Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%). A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001) while similar number of pSS patients and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared to controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison to the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.

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