Application of SSR Markers for Genetic Purity Analysis of Parental Inbred Lines and Some Commercial Hybrid Maize (Zea mays L.)

Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. Marker-assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. In this paper, the relationships between SSR markers and 26 quantitative traits of hybrid maize varieties (Zea mays L.) were analyzed. Association analyses were performed based on 30 SSR primers in a set of thirteen hybrid maize varieties. A total of 112 SSR markers were detected in these genotypes. The number of alleles per locus ranged from 1 to 17, with the average number of alleles per locus equal to 3.7. The number of molecular markers associated with observed traits ranged from 1 (for the number of kernels in row, ears weight and fresh weight of one plant) to 14 (for damage of maize caused by P. nubilalis) in 2016 as well as from 1 (for soil plant analysis development—SPAD, the number of grains in ear and fresh weight of one plant) to 12 (for carotenoids content) in 2017. The sum of statistically significant associations between SSR markers and at least one trait was equal to one hundred sixty in 2016 as well as one hundred twenty-five in 2017. Marker trait associations (MTAs) were found on the basis of regression analysis. The proportion of the total phenotypic variances of individual traits explained by the marker ranged from 24.4% to 77.7% in the first year of study and from 24.3% to 77.9% in 2017. Twenty-two SSR markers performed a significant effect on at least one tested trait in both years of experiment. The three markers (phi021/4, phi036/3, and phi061/2) can be a good tool in marker-assisted selection because they allow simultaneous selection for multiple traits in both years of study, such as the number of kernels in row and the number of grains in ear (phi021/4), the number of plant after germination, the number of plants before harvest, and the number of ears (phi036/3), as well as moisture of grain and length of ears (phi061/2).

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Relationship between the performance of parental inbred lines and hybrids for food-feed traits in maize (Zea mays L.) in Ethiopia

Field Crops Research ◽

10.1016/j.fcr.2013.02.008 ◽

2013 ◽

Vol 153 ◽

pp. 86-93 ◽

Cited By ~ 11

Author(s):

Berhanu Tadesse Ertiro ◽

Habtamu Zeleke ◽

Dennis Friesen ◽

Michael Blummel ◽

S. Twumasi-Afriyie

Keyword(s):

Zea Mays ◽

Zea Mays L ◽

Inbred Lines ◽

Parental Inbred

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Analysis of nonadditive protein accumulation in young primary roots of a maize (Zea mays L.) F1-hybrid compared to its parental inbred lines

PROTEOMICS ◽

10.1002/pmic.200800023 ◽

2008 ◽

Vol 8 (18) ◽

pp. 3882-3894 ◽

Cited By ~ 44

Author(s):

Nadine Hoecker ◽

Tobias Lamkemeyer ◽

Barbara Sarholz ◽

Anja Paschold ◽

Claudia Fladerer ◽

...

Keyword(s):

Zea Mays ◽

Zea Mays L ◽

Inbred Lines ◽

Protein Accumulation ◽

F1 Hybrid ◽

Primary Roots ◽

Parental Inbred

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Microsatellite megatracts in the maize (Zea mays L.) genome

Genome ◽

10.1139/g05-061 ◽

2005 ◽

Vol 48 (6) ◽

pp. 1061-1069 ◽

Cited By ~ 12

Author(s):

E V Ananiev ◽

M A Chamberlin ◽

J Klaiber ◽

S Svitashev

Keyword(s):

Zea Mays ◽

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Ssr Markers ◽

Zea Mays L ◽

Size Variation ◽

Inbred Lines ◽

Retrotransposable Elements ◽

Dynamic Mutations

Long tracts (megatracts) of (CAG)n, (TAG)n, and (GAA)n microsatellite sequences capable of forming composite DNA segments were found in the maize (Zea mays L.) genome. Some of the (CAG)n and (TAG)n megatracts were organized in clusters of up to 1 Mb on several chromosomes, as detected by fluorescence in situ hybridization (FISH), as well as on extended DNA fibers. Extensive polymorphism was found among different maize inbred lines with respect to the number and size of microsatellite megatract clusters on the A chromosomes. Polymorphism was also common among B chromosomes of different nuclei in the inbred line Zapalote Chico. Different retrotransposable elements were often inserted into the microsatellite tracts. Size variation in some (TAG)n and (GAA)n megatracts was observed in consecutive generations among siblings of the inbred lines, indicating that these loci are highly unstable and predisposed to dynamic mutations similar to those described in mammalian systems.Key words: maize, microsatellites, SSR, markers, FISH.

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