Adsorption of Enzyme on Modified Diatomite by Organo-Functional Group and Metallic Ions

In this paper, two calcined Zhejiang diatomite and Jilin diatomite were modified by organic amine, polyethylene glycol, organocationic ions, organofunction group and metallic ions to adsorption and immobilize enzyme, the different organo-function group and metallic ions lead to the capacity of adsorptive enzyme changing with the variation of IEP value.

Immobilization of the Horseradish Peroxide on the Multi-Wall Nanotube and its Application for H2O2 Sensing

In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANIMWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.