Structure-function analysis of the maize bulliform cell cuticle and its role in dehydration and leaf rolling

The cuticle is a hydrophobic layer on the outer surface plant shoots, which serves as an important interaction interface with the environment. It consists of the lipid polymer cutin, embedded with and covered by waxes, and provides protection against stresses including desiccation, UV radiation, and pathogen attack. Bulliform cells form in longitudinal strips on the adaxial leaf surface, and have been implicated in the leaf rolling response observed in drought stressed grass leaves. In this study, we show that bulliform cells of the adult maize leaf epidermis have a specialized cuticle, and we investigate its function along with that of bulliform cells themselves. Analysis of natural variation was used to relate bulliform strip pattering to leaf rolling rate, providing evidence of a role for bulliform cells in leaf rolling. Bulliform cells displayed increased shrinkage compared to other epidermal cell types during dehydration of the leaf, providing a potential mechanism to facilitate leaf rolling. Comparisons of cuticular conductance between adaxial and abaxial leaf surfaces, and between bulliform-enriched mutants vs. wild type siblings, provided evidence that bulliform cells lose water across the cuticle more rapidly than other epidermal cell types. Bulliform cell cuticles have a distinct ultrastructure, and differences in cutin monomer content and composition, compared to other leaf epidermal cells. We hypothesize that this cell type-specific cuticle is more water permeable than the epidermal pavement cell cuticle, facilitating the function of bulliform cells in stress-induced leaf rolling observed in grasses.One sentence summaryBulliform cells in maize have a specialized cuticle, lose more water than other epidermal cell types as the leaf dehydrates, and facilitate leaf rolling upon dehydration.

Phytolith assemblages in modern top soils under plant communities of Northern and Western Altay, Russia

We investigated 120 assemblages of phytoliths from modern top soils of 40 different plant communities of Northern and Western Altay region of Russia. The samples were collected from elevations between 360 m and 2360 m above sea level. Using statistical analyses, it was discovered that many communities produce sufficiently distinct assemblages based on standard morphotypes. Specifically we studied 6 kinds of forests (larch, spruce, fir, pine, cedar pine and birch-dominated), 3 kinds of steppes (true, meadow and petrophytic), 5 kinds of meadows (steppe-like, upland dry, wet floodplain, subalpine, alpine), alpine tundra, and mountain shrubland communities. The communities were not evenly sampled, with more redundancy in some types than in others. Using PCA, it was possible to reveal the few morphotypes most responsible for distinguishing different communities, e.g., low conical rondels, rondel sum, long cell sum, lanceolate cells with massive base, and bulliform cell sum.

Holocene history of intentional fires and grassland development on the Soni Plateau, Central Japan, reconstructed from phytolith and macroscopic charcoal records within cumulative soils, combined with paleoenvironmental data from mire sediments

Phytolith and macroscopic charcoal in cumulative soils on the Soni Plateau, Central Japan, were evaluated to clarify the Holocene history of intentional fires and grassland development, and to compare the findings with those derived from pollen and charcoal records in sediments taken from a nearby mire in the previous study. Prior to ~1500 cal. BP, Bambusoid short-cell phytoliths and Pleioblastus-type and Bambusoideae-type bulliform cell phytoliths were abundant with scarce charcoal particles (<1000 particles/cm3). In contrast, since ~1500 cal. BP Andropogoneae-type bulliform cell phytoliths and Bilobate short-cell phytoliths were dominant with abundant charcoal particles (>1000 particles/cm3). Based on correlating these records with pollen and charcoal records in mire sediments, prior to ~1500 cal. BP, dwarf bamboo flourished on the forest floor under largely fire-free conditions, whereas since ~1500 cal. BP, grassland dominated by Japanese pampas grass has been sustained by periodic intentional burning that has continued until the present day.