highly repeated dna
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Genome ◽  
2002 ◽  
Vol 45 (5) ◽  
pp. 973-976
Author(s):  
Michele Boniotto ◽  
Mario Ventura ◽  
Maria Francesca Cardone ◽  
Francesca Boaretto ◽  
Nicoletta Archidiacono ◽  
...  

We have isolated and cloned an 800-bp highly repeated DNA (HRDNA) sequence from Lemur catta (LCA) and described its localization on LCA chromosomes. Lemur catta HRDNA sequences were localized by performing FISH experiments on standard and elongated metaphasic chromosomes using an LCA HRDNA probe (LCASAT). A complex hybridization pattern was detected. A strong pericentromeric hybridization signal was observed on most LCA chromosomes. Chromosomes 7 and 13 were lit in pericentromeric regions, as well as in the interspersed heterochromatin. Chromosomes 1, 3, 4, 17, 19, X, and microchromosomes (20, 25, 26, and 27) showed no signals in the pericentromeric region, but chromosomes 3 and 4 showed a positive hybridization in heterochromatic regions. The 800-bp L. catta HRDNA was species specific. We performed FISH experiments with the LCASAT probe on Eulemur macaco macaco (EMA) andEulemur fulvus fulvus (EFU) metaphases and no positive signal of hybridization was detected. These findings were also confirmed by Southern blot analysis and PCR.Key words: highly repeated DNA, FISH, chromosome rearrangements, heterochromatin, Malagasy lemurs.


Heredity ◽  
2002 ◽  
Vol 88 (5) ◽  
pp. 366-370 ◽  
Author(s):  
M J L Barragán ◽  
S Martínez ◽  
J A Marchal ◽  
M Bullejos ◽  
R Díaz de La Guardia ◽  
...  

Heredity ◽  
2001 ◽  
Vol 87 (6) ◽  
pp. 637-646 ◽  
Author(s):  
Rosa Fernández ◽  
María José L Barragán ◽  
Mónica Bullejos ◽  
Juan Alberto Marchal ◽  
Sergio Martínez ◽  
...  

Gene ◽  
2001 ◽  
Vol 275 (2) ◽  
pp. 305-310 ◽  
Author(s):  
Mario Ventura ◽  
Michele Boniotto ◽  
Maria Francesca Cardone ◽  
Luigi Fulizio ◽  
Nicoletta Archidiacono ◽  
...  

Chromosoma ◽  
1999 ◽  
Vol 108 (7) ◽  
pp. 436-442 ◽  
Author(s):  
M. Mandrioli ◽  
D. Bizzaro ◽  
G. C. Manicardi ◽  
D. Gionghi ◽  
L. Bassoli ◽  
...  

1998 ◽  
Vol 15 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Marco Passamonti ◽  
Barbara Mantovani ◽  
Valerio Scali

1998 ◽  
Vol 15 (4) ◽  
pp. 599-605 ◽  
Author(s):  
Marco Passamonti ◽  
Barbara Mantovani ◽  
Valerio Scali

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