Localization of a new highly repeated DNA sequence of Lemur catta (Lemuridae, Strepsirhini)

Genome ◽  
2002 ◽  
Vol 45 (5) ◽  
pp. 973-976
Author(s):  
Michele Boniotto ◽  
Mario Ventura ◽  
Maria Francesca Cardone ◽  
Francesca Boaretto ◽  
Nicoletta Archidiacono ◽  
...  

We have isolated and cloned an 800-bp highly repeated DNA (HRDNA) sequence from Lemur catta (LCA) and described its localization on LCA chromosomes. Lemur catta HRDNA sequences were localized by performing FISH experiments on standard and elongated metaphasic chromosomes using an LCA HRDNA probe (LCASAT). A complex hybridization pattern was detected. A strong pericentromeric hybridization signal was observed on most LCA chromosomes. Chromosomes 7 and 13 were lit in pericentromeric regions, as well as in the interspersed heterochromatin. Chromosomes 1, 3, 4, 17, 19, X, and microchromosomes (20, 25, 26, and 27) showed no signals in the pericentromeric region, but chromosomes 3 and 4 showed a positive hybridization in heterochromatic regions. The 800-bp L. catta HRDNA was species specific. We performed FISH experiments with the LCASAT probe on Eulemur macaco macaco (EMA) andEulemur fulvus fulvus (EFU) metaphases and no positive signal of hybridization was detected. These findings were also confirmed by Southern blot analysis and PCR.Key words: highly repeated DNA, FISH, chromosome rearrangements, heterochromatin, Malagasy lemurs.

1986 ◽  
Vol 204 (3) ◽  
pp. 417-423 ◽  
Author(s):  
Jose M. Martinez-Zapater ◽  
Mark A. Estelle ◽  
Chris R. Somerville

1985 ◽  
Vol 13 (6) ◽  
pp. 1965-1975 ◽  
Author(s):  
Ramareddy V. Guntaka ◽  
Siddarame Gowda ◽  
Arepalli S. Rao ◽  
Theodore J. Green

1989 ◽  
Vol 78 (4) ◽  
pp. 589-593 ◽  
Author(s):  
N. V. Sonina ◽  
A. A. Lushnikova ◽  
A. P. Tihonov ◽  
E. V. Ananiev

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1237-1243 ◽  
Author(s):  
K. Hirai ◽  
K. Irifune ◽  
R. Tanaka ◽  
H. Morikawa

A highly repeated DNA sequence with a repeat unit of ca. 180 bp was found in genomic DNA HindIII-digests of Raphanus sativus. The repeating units of six isolated, independent clones were sequenced. These units have 177 or 178 bp, are 36% G+C in their DNA base composition, and show 90% sequence homology. The copy number of this 180-bp repeat unit is about 0.5 × 106 per diploid genome. In situ hybridization analysis with the repeating unit as the probe and C-banding analysis indicated that the repeated DNA sequence of R. sativus is closely associated with the major C-heterochromatins in the proximal regions of all 18 chromosomes at mitotic metaphase.Key words: Raphanus sativus, repeated DNA sequence, nucleotide sequence, in situ hybridization, C-banding.


Chromosoma ◽  
1999 ◽  
Vol 108 (7) ◽  
pp. 436-442 ◽  
Author(s):  
M. Mandrioli ◽  
D. Bizzaro ◽  
G. C. Manicardi ◽  
D. Gionghi ◽  
L. Bassoli ◽  
...  

1987 ◽  
Vol 3 (12) ◽  
pp. 378-379 ◽  
Author(s):  
W.F. Piessens ◽  
L.A. McReynolds ◽  
S.A. Williams

1993 ◽  
Vol 111 (2-3) ◽  
pp. 147-152 ◽  
Author(s):  
T.J. Doran ◽  
A.L.M. Hodgson ◽  
J.K. Davies ◽  
A.J. Radford

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