Temporary adhesion of the proseriate flatworm Minona ileanae

Flatworms can very rapidly attach to and detach from many substrates. In the presented work, we analysed the adhesive system of the marine proseriate flatworm Minona ileanae . We used light-, scanning- and transmission electron microscopy to analyse the morphology of the adhesive organs, which are located at the ventral side of the tail-plate. We performed transcriptome sequencing and differential RNA-seq for the identification of tail-specific transcripts. Using in situ hybridization expression screening, we identified nine transcripts that were expressed in the cells of the adhesive organs. Knock-down of five of these transcripts by RNA interference led to a reduction of the animal's attachment capacity. Adhesive proteins in footprints were confirmed using mass spectrometry and antibody staining. Additionally, lectin labelling of footprints revealed the presence of several sugar moieties. Furthermore, we determined a genome size of about 560 Mb for M. ileanae . We demonstrated the potential of Oxford Nanopore sequencing of genomic DNA as a cost-effective tool for identifying the number of repeats within an adhesive protein and for combining transcripts that were fragments of larger genes. A better understanding of the molecules involved in flatworm bioadhesion can pave the way towards developing innovative glues with reversible adhesive properties. This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’.

Echinochiton dufoei: A New Spiny Ordovician chiton

Echinochiton dufoei new genus and species is described from the Ordovician age Forreston Member, Grand Detour Formation (Blackriveran) near Beloit, Wisconsin. For a variety of reasons, we regard E. dufoei as a chiton; the species is known from four articulated or partially articulated specimens, one of which has eight plates and two of which have a mucro on the tail plate. Echinochiton dufoei differs from other chitons in having large hollow spines that project from each of the known plates. In plate shape and position, E. dufoei is much like the Upper Cambrian species Matthevia variabilis Walcott, 1885, and the Lower Ordovician species Chelodes whitehousei Runnegar, Pojeta, Taylor, and Collins (1979).

The aglaspidid arthropod Beckwithia from the Cambrian of Utah and Wisconsin

New specimens of Beckwithia typa, from the upper Middle Cambrian of Utah, show that, contrary to previous descriptions, the animal had at least 10 tergites (possibly 12) and probably a tail-spine. There is no evidence for a fused tail-plate, the one character that made Beckwithia appear anomalous with respect to other aglaspidid arthropods. It did, however, differ from most other aglaspidids in having a single series of axial spines along the trunk, but this cannot be regarded as a basis for continued separation of Beckwithia into a monogeneric family. A possible relationship with Kodymirus vagans is suggested by the presence of axial spines, although there may have been significant differences in the nature of the ventral sclerites. Beckwithia? major from the Upper Cambrian of Wisconsin is known only from fragmentary specimens and no evidence of a fused tail-plate has been found. Beckwithia? daubikhensis from the Khanka Massif, Soviet Union, is unlikely to be a member of this genus; the single specimen on which the taxon was based may be a poorly preserved Khankaspis bazhanovi.