Application of Date Seeds Powder as Growth Additive for Callus Induction in vitro Using Vigna radiata Hypocotyl Seedling Explant

Date seeds (Phoenix dectylifera) are one of the seeds that is not usable and always end to be disposed. The present study describes date seeds as an additive on callus induction in vitro from hypocotyl explants of Vigna radiata. The objective is to explore the usage of date seed powder as growth additive to promote in plant tissue culture media thereby it can be utilized as fertilizer in vivo for sustainable agriculture. 1% concentrations of date seed powder under controlled conditions highly influenced the callus induction. MS media supplied with different auxins is prepared for callus induction. The highest degree of callus weight was observed in MS media supplemented with (5mg/L) 2, 4-D + (0.5 mg/L) Kn (0.437±0.1). MS media supplied with different concentrations of date seed powder + (5mg/L) 2,4-D + (0.5 mg/L) Kn are prepared for callus induction under controlled conditions (16hrs light and 8hrs dark, 3000 lux light intensity,60% humidity and 25±20c) in a plant growth chamber. MS media supplied with 1% date seed + 3% sucrose + 0.5 mg/l of Kn +5 mg/l of 2,4D gave the highest stimulation of callus growth. Results show that date seed is not replacing sucrose as a carbon source, but it acts as a good additive to promote induction callus. Quantitative nutritional analysis of date seed powder was carried out. The results show date seed powder contains a high amount of elements like: Ca (2994.33), k (1712.33), Si (456.33), Mg (687.33), which plays a major role in callus formation.

Direct shoot regeneration from epicotyl explants of grasspea (Lathyrus sativus)

A reproducible procedure is described for adventitious shoot organogenesis in epicotyl segments resulting in prolific plant regeneration of a grain legume grasspea (Lathyrus sativus L.). Among seedling explant types examined, epicotyl segments were most responsive. The highest percentage of direct shoot regeneration was elicited on Murashige–Skoog (MS) medium augmented with 4.0 mg L–1 6-benzyladenine (BA) + 2.0 mg L–1 α-naphthaleneacetic acid (NAA). Compared with four other genotypes examined, IC-120487 showed the highest shoot regeneration frequency (approximately 80%) with maximum shoot numbers (averaging eight shoots per explant) and longest average shoot length (approximately 4 cm). Rhizogenesis was induced in ~78% of the regenerated shoots in half-strength MS medium containing 0.5 mg L–1 indole-3-acetic acid (IAA). Plantlets were acclimated in vermi-compost and 75% of those transferred to soil survived and set viable seeds.