Direct shoot regeneration from epicotyl explants of grasspea (Lathyrus sativus)

A reproducible procedure is described for adventitious shoot organogenesis in epicotyl segments resulting in prolific plant regeneration of a grain legume grasspea (Lathyrus sativus L.). Among seedling explant types examined, epicotyl segments were most responsive. The highest percentage of direct shoot regeneration was elicited on Murashige–Skoog (MS) medium augmented with 4.0 mg L–1 6-benzyladenine (BA) + 2.0 mg L–1 α-naphthaleneacetic acid (NAA). Compared with four other genotypes examined, IC-120487 showed the highest shoot regeneration frequency (approximately 80%) with maximum shoot numbers (averaging eight shoots per explant) and longest average shoot length (approximately 4 cm). Rhizogenesis was induced in ~78% of the regenerated shoots in half-strength MS medium containing 0.5 mg L–1 indole-3-acetic acid (IAA). Plantlets were acclimated in vermi-compost and 75% of those transferred to soil survived and set viable seeds.

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Induction of Direct Adventitious Shoot Regeneration in Pear (Pyrus communis L.)

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v24i1.19215 ◽

2014 ◽

Vol 24 (1) ◽

pp. 87-92 ◽

Cited By ~ 2

Author(s):

Mahdieh Yousefiara ◽

Maryam Jafarkhani Kermani ◽

Abdolreza Bagheri ◽

Ali Akbar Habashi ◽

Hamid Abdollahi

Keyword(s):

Shoot Regeneration ◽

Culture Media ◽

Adventitious Shoot ◽

Pyrus Communis ◽

Explant Type ◽

Direct Shoot Regeneration ◽

Adventitious Shoot Regeneration ◽

Engineering Research ◽

Pyrus Communis L ◽

Direct Shoot

Efficient direct shoot regeneration of two pear (Pyrus communis L.), namely 'bartlett' and 'dargazi' was successfully developed for the use in future genetic engineering research. The basal MS and NN media supplemented with different concentrations of TDZ (0, 2.5, 5, 7.5 ?M) or BAP (0, 4, 8, 16 ?M) in combination with NAA (1 ?M) were compared. The result showed that direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media. 'dargazi' had higher rate of regeneration compared to 'bartlett' and in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves. Although the highest per cent of regeneration in 'bartlett' (38) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, but the differences in shoot regeneration between media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest per cent of regeneration in 'dargazi' (56) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA. Plant Tissue Cult. & Biotech. 24(1): 87-92, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19215

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Direct shoot regeneration from male immature flower buds of Musa paradisiaca Linn. cv. Poovan (AAB)

Plant Science Today ◽

10.14719/pst.2018.5.4.403 ◽

2018 ◽

Vol 5 (4) ◽

pp. 142-148

Author(s):

Anjana R G Nair ◽

P Ravichandran ◽

Mathew Bejoy

Keyword(s):

Shoot Regeneration ◽

Male Flower ◽

Basal Region ◽

Ms Medium ◽

Direct Shoot Regeneration ◽

Benzyl Adenine ◽

Flower Buds ◽

Flower Bud ◽

Musa Paradisiaca ◽

Direct Shoot

A tissue culture system has been developed to multiply Musa paradisiaca cv. Poovan using male immature flower bud and to establish it in ex vitro condition. Size of explants has been found an influencing factor for culture initiation. Immature male flower bud segments of 3 cm size were ideal for better survival and subsequent shoot regeneration. Direct shoot regeneration was achieved from male immature flower buds on Murashige and Skoog (MS) medium supplemented with varying concentrations of plant growth regulators. Initially, actively dividing meristematic region developed at the basal region of flower buds near the bract axil, which later grew into green shoot buds in most of the PGR treatments. Single use of benzyl adenine were found beneficial than kinetin or addition of indole-3-acetic acid. Maximum production of 31.0 ± 0.65 shoots was achieved on MS + 3% sucrose + 6 mg/L benzyl adenine in 15 weeks. Isolated healthy shoots were rooted in half-strength MS medium with 150 mg/L activated charcoal + 30 g/L sucrose + 1 mg/L indole-3-butyric acid within 15 days and they established successfully in greenhouse conditions with 85 % survival.

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High frequency direct shoot regeneration from Kazakh commercial potato cultivars

PeerJ ◽

10.7717/peerj.9447 ◽

2020 ◽

Vol 8 ◽

pp. e9447

Author(s):

Laura S. Abeuova ◽

Balnur R. Kali ◽

Aizhan O. Rakhimzhanova ◽

Sara S. Bekkuzhina ◽

Shuga A. Manabayeva

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Multiple Shoot ◽

Potato Cultivars ◽

Direct Regeneration ◽

Naphthaleneacetic Acid ◽

Direct Shoot Regeneration ◽

Potato Genome ◽

Direct Shoot

Potato (Solanum tuberosum L.) is the third most economically important crop in the world and has a high nutritional value. In this study, the in vitro culture response of four widely grown in Kazakhstan potato cultivars, Astanalyk, Monument Kunaev, Tokhtar, and Aksor, was investigated using stem and leaf explants. Published protocols were evaluated and optimized to develop a more efficient protocol for the regeneration of plants from local potato cultivars in tissue culture, which is a prerequisite to facilitate potato genome modification. The explants were cultured on solid Murashige and Skoog medium supplemented with different concentrations and combinations of zeatin, 6-benzylaminopurine (BAP), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA). The maximum regeneration was induced from the stem internodal explants. A significant effect of the explant source on direct regeneration was confirmed with statistical analysis. The number of shoots obtained from the internode was 10.0 from cv. Aksor followed by cvs. Tokhtar and Astanalyk. The medium DRM-VIII with 1 mg/l zeatin, 0.1 mg/l IAA and 7.0 mg/l GA3 was considered the best for direct shoot regeneration and multiple shoot formation from all cultivars. To conclude, we outline a protocol for direct plant regeneration from four potato cultivars. Our findings suggest commercial cultivars Astanalyk and Aksor are good candidates for developing the genome-edited plants through direct shoot regeneration.

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Direct shoot regeneration from immature inflorescence cultures of Chlorophytum arundinaceum and Chlorophytum borivilianum

Biologia ◽

10.2478/s11756-009-0039-1 ◽

2009 ◽

Vol 64 (2) ◽

Cited By ~ 3

Author(s):

Sanghamitra Samantaray ◽

Saroj Kumar ◽

Satyabrata Maiti

Keyword(s):

Shoot Regeneration ◽

Terminal Segment ◽

Type I ◽

Ms Medium ◽

Direct Shoot Regeneration ◽

Immature Inflorescence ◽

Shoot Buds ◽

Shoot Bud ◽

Half Strength ◽

Direct Shoot

AbstractDirect shoot regeneration was achieved from immature inflorescence explants of Chlorophytum arundinaceum and C. borivilianum on half-strength Murashige & Skoog (MS) medium supplemented with 3.0 mg L−1 BA, 150 mg L−1 Ads, 0.1 mg L−1 NAA and 3% (w/v) sucrose under a 16-h photoperiod. The shoot buds developed within 2–3 weeks of culture. High frequency of shoot bud regeneration was achieved when cultured on similar medium in subsequent subcultures. The apex portion (Type I) of the inflorescence produced more shoot buds as compared to the middle ones (type II). More than 75% of the terminal segment explants produced shoot buds within 4-week of culture. Response of basal portion (Type III) was negative for shoot bud initiation. Shoots rooted on half-strength basal MS medium supplemented with half-strength MS medium, 0.1 mg L−1 IAA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house and successfully established in the soil where 90% of the plants survived. This protocol would be useful for commercial micropropagation and genetic improvement prograrmme.

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The Effect of Pretreating Seedlings with Bap on Direct Shoot Regeneration from Petiole Explants of Sugar Beet (Beta VulgarisL.)

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2003.10817064 ◽

2003 ◽

Vol 17 (2) ◽

pp. 89-96 ◽

Cited By ~ 5

Author(s):

E. Gürel ◽

E. Topal ◽

S. Gürel

Keyword(s):

Sugar Beet ◽

Shoot Regeneration ◽

Direct Shoot Regeneration ◽

Petiole Explants ◽

Direct Shoot

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Direct shoot regeneration from leaf explants of Rosa damascena Mill.

In Vitro Cellular & Developmental Biology - Plant ◽

10.1079/ivp2003503 ◽

2004 ◽

Vol 40 (2) ◽

pp. 192-195 ◽

Cited By ~ 23

Author(s):

Pratap Kumar Pati ◽

Madhu Sharma ◽

Anil Sood ◽

P. S. Ahuja

Keyword(s):

Shoot Regeneration ◽

Leaf Explants ◽

Rosa Damascena ◽

Direct Shoot Regeneration ◽

Direct Shoot

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Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

Glasnik Sumarskog fakulteta ◽

10.2298/gsf1206141m ◽

2012 ◽

pp. 141-150

Author(s):

Marija Markovic ◽

Milos Tanasic ◽

Nevena Stojic ◽

Radivoje Bulatovic ◽

Marta Jovic ◽

...

Keyword(s):

Shoot Regeneration ◽

Coconut Water ◽

Soy Flour ◽

Multiplication Rate ◽

Direct Shoot Regeneration ◽

Medium Components ◽

Cultivation In Vitro ◽

Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

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