scholarly journals Application of Date Seeds Powder as Growth Additive for Callus Induction in vitro Using Vigna radiata Hypocotyl Seedling Explant

2020 ◽  
pp. 11-21
Author(s):  
Salla Hemadri Reddy ◽  
Ibtihal Sultan Al Maskari ◽  
Shaima Eid Alrubkhi ◽  
Shamsa Sulaiman Alkindi
Keyword(s):  
Callus Induction ◽  
Vigna Radiata ◽  
Culture Media ◽  
Callus Formation ◽  
Date Seed ◽  
Tissue Culture Media ◽  
Controlled Conditions ◽  
Seedling Explant

Date seeds (Phoenix dectylifera) are one of the seeds that is not usable and always end to be disposed. The present study describes date seeds as an additive on callus induction in vitro from hypocotyl explants of Vigna radiata. The objective is to explore the usage of date seed powder as growth additive to promote in plant tissue culture media thereby it can be utilized as fertilizer in vivo for sustainable agriculture. 1% concentrations of date seed powder under controlled conditions highly influenced the callus induction. MS media supplied with different auxins is prepared for callus induction. The highest degree of callus weight was observed in MS media supplemented with (5mg/L) 2, 4-D + (0.5 mg/L) Kn (0.437±0.1). MS media supplied with different concentrations of date seed powder + (5mg/L) 2,4-D + (0.5 mg/L) Kn are prepared for callus induction under controlled conditions (16hrs light and 8hrs dark, 3000 lux light intensity,60% humidity and 25±20c) in a plant growth chamber.  MS media supplied with 1% date seed + 3% sucrose + 0.5 mg/l of Kn +5 mg/l of 2,4D gave the highest stimulation of callus growth. Results show that date seed is not replacing sucrose as a carbon source, but it acts as a good additive to promote induction callus. Quantitative nutritional analysis of date seed powder was carried out. The results show date seed powder contains a high amount of elements like: Ca (2994.33), k (1712.33), Si (456.33), Mg (687.33), which plays a major role in callus formation.

scholarly journals Induced callus from seedlings of Peganum harmala L. and studying harmine compound concentration in vitro and in vivo by GC analysis

2019 ◽  
pp. 1442-1451
Author(s):  
H. H. Mutasher ◽  
H. J. Attiya
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Culture Media ◽  
Dry Weight ◽  
Peganum Harmala ◽  
Tissue Culture Media ◽  
Compound Concentration ◽  
Stimulation Of

Plant tissue culture considers a benefit biotechnological technique for scientific research especially the production of undifferentiation callus cells and regeneration through suspension or static media. The seedlings of Peganum harmala was used as a source to produce callus mass in vitro in static media through different tissue culture media supplemented by varying combinations of plant growth regulators (PGR). The result illustrates that 2 mg/l of Kinitine with 0.5 mg/l of 2, 4-D was efficient to stimulate callus induction with percent 100% in stem and root of P. harmala and this combination gave a high fresh weight, 1954 mg in root and 1170mg in stem and high dry weight in root and stem was 74.60, 60.30 respectively. In a comparative analysis through gas chromatography (GC) the stem and root in field recorded harmine concentration 56.13 and 40.95 μg respectively, which was higher than the in vitro callus induction from stem and root, which may be due to the fact that field plants have not been exposed to plant hormones with concentrations higher than the normal level, which reduced the stimulation of cells producing active compounds.

scholarly journals IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH

2018 ◽  
Vol 10 (9) ◽  
pp. 68
Author(s):  
Deep Chhavi Anand ◽  
Rishikesh Meena ◽  
Vidya Patni
Keyword(s):  
Callus Induction ◽  
Culture Media ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Dodecanoic Acid ◽  
Stem Explants ◽  
Ms Analysis ◽  
Wide Range

Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.

scholarly journals Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Pemberian ZPT 2,4-D (Dichlorophenoxy Acetid Acid)

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Siti RLR Idris ◽  
Asri Pirade Paserang
Keyword(s):  
Solanum Tuberosum ◽  
Callus Induction ◽  
Culture Media ◽  
Callus Formation ◽  
Solanum Tuberosum L ◽  
Randomized Design ◽  
Completely Randomized Design

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

scholarly journals Effect of cisplatin on the plasma membrane phosphatase activities in ascites sarcoma-180 cells: a cytochemical study.

1983 ◽  
Vol 31 (2) ◽  
pp. 307-317 ◽  
Author(s):  
S K Aggarwal ◽  
I Niroomand-Rad
Keyword(s):  
Alkaline Phosphatase ◽  
Plasma Membrane ◽  
Culture Media ◽  
Tumor Burden ◽  
Sarcoma 180 ◽  
Cytochemical Study ◽  
In Vivo Experiments ◽  
Tissue Culture Media

To study the effects of cisplatin [cis-dichlorodiammine-platinum (II)] on tumor cells in the presence or absence of the immune system, animals with ascites sarcoma-180 tumor burden were treated with therapeutic dose levels (9 mg/kg). Similarly, ascites sarcoma-180 cells were maintained in tissue culture media containing the same levels of the drug. Cell samples were taken from the animals at 12-hr intervals for 3 days, whereas samples were drawn from the tissue cultures at 15-, 30-, 45-, and 60-min and at 2-, 3-, 4-, and 5-hr intervals. Treated and untreated cells from in vitro and in vivo experiments, when checked for alkaline phosphatase, 5'-nucleotidase, Ca2+-ATPase, and Na+-K+-ATPase, show a gradual decrease in activity on the plasma membrane. It takes about 60 min for inactivation of any enzyme in vitro, whereas it takes 2 days in in vivo experiments. Quantitative analysis show alkaline phosphatase activity drops from 9.7 to 4.9 nmol in just 15 min, and drops further to 0.79 nmol after 2 hr. Inactivation of various plasma membrane enzymes, resulting in permeability changes, is probably responsible for cell death.

scholarly journals Sirtuin 1 Modulation in Rat Model of Acetaminophen-Induced Hepatotoxicity

2015 ◽  
pp. S477-S487 ◽  
Author(s):  
L. WOJNAROVÁ ◽  
N. KUTINOVÁ CANOVÁ ◽  
H. FARGHALI ◽  
T. KUČERA
Keyword(s):  
Culture Media ◽  
Specific Treatment ◽  
Sirtuin 1 ◽  
Drug Induced ◽  
Tissue Culture Media ◽  
Male Wistar Rats ◽  
Apoptosis And Inflammation

Sirtuin 1 (SIRT1) is involved in important biological processes such as energy metabolism and regulatory functions of the cell cycle, apoptosis, and inflammation. Our previous studies have shown hepatoprotective effect of polyphenolic compound resveratrol, which is also an activator of SIRT1. Therefore, the aim of our present study was to clarify the role of SIRT1 in process of hepatoprotection in animal model of drug-induced liver damage. Male Wistar rats were used for both in vivo and in vitro studies. Hepatotoxicity was induced by single dose of acetaminophen (APAP). Some rats and hepatocytes were treated by resveratrol or synthetic selective activator of sirtuin 1 (CAY10591). The degree of hepatotoxicity, the activity and expression of the SIRT1 were determined by biochemical, histological and molecular-biological assessments of gained samples (plasma, liver tissue, culture media and hepatocytes). Resveratrol and CAY attenuated APAP-induced hepatotoxicity in vivo and in vitro. Moreover, both drugs enhanced APAP-reduced SIRT1 activity. Our results show that modulation of the SIRT1 activity plays a role in hepatoprotection. Synthetic activators of SIRT1 would help in understanding the role of SIRT1 and are therefore a major boost towards the search for specific treatment of liver disease.

scholarly journals Prooxidative and antioxidative properties of cucumber (Cucumis sativus L.) callus in vitro and young in vivo plantlets in response to copper ions

2013 ◽  
Vol 25 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Alexander S. Lukatkin ◽  
Irina D. Michailova ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Oxidative Stress ◽  
Cucumis Sativus ◽  
Callus Induction ◽  
Copper Ions ◽  
Callus Formation ◽  
Negative Influence ◽  
Cucumis Sativus L ◽  
Biochemical Indices

ABSTRACT The effects of different concentrations of copper ions (Cu2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. ‘Edinstvo’) seedlings as well as on in vitro hypocotyl-derived callus were considered. Callus induction from hypocotyls was more prolific than from roots or cotyledons. Thus, callus obtained from hypocotyls of 7-day-old cucumber plants was cultured for 5 weeks on Murashige and Skoog medium containing 4 mg dm-3 2,4-D + 1 mg dm-3 BA supplemented with 0.01 mM, 0.1 mM or 1.0 mM of Cu2+. Biochemical indices related to oxidative stress were assessed. Cu2+ at 0.01 mM stimulated callus induction but 1.0 mM Cu2+ negatively affected callus formation and growth. LPO intensity was significantly lower than the control at all concentrations of Cu2+ but significantly higher than the control in plants exposed to 0.01 or 0.1 mM Cu2+. A similar trend was observed for the generation of the superoxide radical in both callus and plantlets. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APOX) activity increased in both callus and plantlets, but the level of increase in these antioxidant enzyme systems depended on the Cu2+ concentration. Cu2+ ions had a stronger (i.e., more negative) influence on oxidative stress in cucumber seedlings than on cucumber callus.

Stress response inhibits the nephrotoxicity of cisplatin

2005 ◽  
Vol 288 (1) ◽  
pp. F125-F132 ◽  
Author(s):  
Marie H. Hanigan ◽  
Mei Deng ◽  
Lei Zhang ◽  
Peyton T. Taylor ◽  
Maia G. Lapus
Keyword(s):  
Tissue Culture ◽  
Stress Response ◽  
Culture Media ◽  
Sodium Concentration ◽  
Vitro Assay ◽  
Salt Loading ◽  
Cisplatin Nephrotoxicity ◽  
Tissue Culture Media

Salt loading and saline hydration are used to protect patients from cisplatin-induced nephrotoxicity. The mechanism by which salt exerts its protective effect is unknown. As part of an ongoing study of cisplatin nephrotoxicity, an in vitro assay system was developed that models the in vivo exposure and response of proximal tubule cells to cisplatin. In this study, it was discovered that the toxicity of cisplatin toward LLC-PK1 cells varied dramatically according to the tissue culture media used for 3-h cisplatin exposure. Further experiments revealed that minor variations in the sodium concentration among standard tissue culture media modulated cisplatin nephrotoxicity. NaCl has been shown to protect against cisplatin-induced nephrotoxicity in vivo but has never before been demonstrated in vitro. NaCl did not alter the cellular accumulation of cisplatin. NaCl altered the osmolarity of the external media, and its effect was replicated by substituting equiosmolar concentrations of impermeant anions or cations. The change in osmolarity triggered a stress response within the cell that modulated sensitivity to cisplatin. These data resolve several long-standing controversies regarding the mechanism by which salt loading protects the kidney from cisplatin-induced nephrotoxicity.

scholarly journals Nanoscale Strontium-Substituted Hydroxyapatite Pastes and Gels for Bone Tissue Regeneration

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1611
Author(s):  
Caroline J. Harrison ◽  
Paul V. Hatton ◽  
Piergiorgio Gentile ◽  
Cheryl A. Miller
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Culture Media ◽  
Full Range ◽  
Sol Gel ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Tissue Culture Media ◽  
Bone Deposition

Injectable nanoscale hydroxyapatite (nHA) systems are highly promising biomaterials to address clinical needs in bone tissue regeneration, due to their excellent biocompatibility, bioinspired nature, and ability to be delivered in a minimally invasive manner. Bulk strontium-substituted hydroxyapatite (SrHA) is reported to encourage bone tissue growth by stimulating bone deposition and reducing bone resorption, but there are no detailed reports describing the preparation of a systematic substitution up to 100% at the nanoscale. The aim of this work was therefore to fabricate systematic series (0–100 atomic% Sr) of SrHA pastes and gels using two different rapid-mixing methodological approaches, wet precipitation and sol-gel. The full range of nanoscale SrHA materials were successfully prepared using both methods, with a measured substitution very close to the calculated amounts. As anticipated, the SrHA samples showed increased radiopacity, a beneficial property to aid in vivo or clinical monitoring of the material in situ over time. For indirect methods, the greatest cell viabilities were observed for the 100% substituted SrHA paste and gel, while direct viability results were most likely influenced by material disaggregation in the tissue culture media. It was concluded that nanoscale SrHAs were superior biomaterials for applications in bone surgery, due to increased radiopacity and improved biocompatibility.

scholarly journals Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 195
Author(s):  
Alla A. Shulgina ◽  
Elena A. Kalashnikova ◽  
Ivan G. Tarakanov ◽  
Rima N. Kirakosyan ◽  
Mikhail Yu. Cherednichenko ◽  
...  
Keyword(s):  
Culture Media ◽  
Stevia Rebaudiana ◽  
Adventitious Shoot ◽  
Optimal Growth ◽  
Medium Composition ◽  
Light Spectra ◽  
Stevia Rebaudiana Bertoni ◽  
Multiplication Coefficient

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs).

scholarly journals Somaclonal variation on in vitro callus culture potato cultivars

2004 ◽  
Vol 22 (2) ◽  
pp. 300-304 ◽  
Author(s):  
Patricia N. Bordallo ◽  
Derly H. Silva ◽  
José Maria ◽  
Cosme D. Cruz ◽  
Elizabeth P. Fontes
Keyword(s):  
Somaclonal Variation ◽  
Culture Media ◽  
Callus Formation ◽  
Potato Cultivars ◽  
Synthetic Seed ◽  
Arbitrary Sequence ◽  
Stem Explants ◽  
Factors Affecting ◽  
High Level

Synthetic seeds can be an alternative for those species in which botanical seeds are not viable. One of the major problems of in vitro plant cultivation is the high level of somaclonal variation. The most common factors affecting somaclonal variation are genotype, explant source, in vitro period and cultivation conditions in which the culture is established. In this work, calli were induced using leaf and stem explants of the commercial potato cultivars Achat, Baraka, Baronesa, Bintje, and Contenda in MS culture media supplemented with 1.65 mM of picloram and 11.5 mM of 2,4-D. Seventy and 90 days after induction, DNA samples of 40 calli were compared concerning the effects of the two explant (leaf and stem) and two growth regulator sources on five potatoes cultivars. A total of 20 arbitrary sequence primers were evaluated. The RAPD pattern generated by these primers suggested a high percentage of polymorphic fragments among the five genotypes, indicating a high level of genetic variation among cultivars. Cultivar Baronesa showed the highest number of polymorphic fragments for all treatments. The cultivar Contenda showed the smallest somaclonal variation, for most of the treatments, except for the treatment which consisted of stem explants, picloram (1.65 mM) application, and a 70-day period of callus formation. 'Contenda' is, therefore, the most suitable cultivar for synthetic seed production.

Sign in / Sign up

Export Citation Format

Share Document