PLSDB: a resource of complete bacterial plasmids

The study of bacterial isolates or communities requires the analysis of the therein included plasmids in order to provide an extensive characterization of the organisms. Plasmids harboring resistance and virulence factors are of especial interest as they contribute to the dissemination of antibiotic resistance. As the number of newly sequenced bacterial genomes is growing a comprehensive resource is required which will allow to browse and filter the available plasmids, and to perform sequence analyses. Here, we present PLSDB, a resource containing 13 789 plasmid records collected from the NCBI nucleotide database. The web server provides an interactive view of all obtained plasmids with additional meta information such as sequence characteristics, sample-related information and taxonomy. Moreover, nucleotide sequence data can be uploaded to search for short nucleotide sequences (e.g. specific genes) in the plasmids, to compare a given plasmid to the records in the collection or to determine whether a sample contains one or multiple of the known plasmids (containment analysis). The resource is freely accessible under https://ccb-microbe.cs.uni-saarland.de/plsdb/.

Cellular and Molecular Heterogeneity Associated with Vessel Formation Processes

The microvasculature heterogeneity is a complex subject in vascular biology. The difficulty of building a dynamic and interactive view among the microenvironments, the cellular and molecular heterogeneities, and the basic aspects of the vessel formation processes make the available knowledge largely fragmented. The neovascularisation processes, termed vasculogenesis, angiogenesis, arteriogenesis, and lymphangiogenesis, are important to the formation and proper functioning of organs and tissues both in the embryo and the postnatal period. These processes are intrinsically related to microvascular cells, such as endothelial and mural cells. These cells are able to adjust their activities in response to the metabolic and physiological requirements of the tissues, by displaying a broad plasticity that results in a significant cellular and molecular heterogeneity. In this review, we intend to approach the microvasculature heterogeneity in an integrated view considering the diversity of neovascularisation processes and the cellular and molecular heterogeneity that contribute to microcirculatory homeostasis. For that, we will cover their interactions in the different blood-organ barriers and discuss how they cooperate in an integrated regulatory network that is controlled by specific molecular signatures.

Symbolic interactionism

Symbolic interactionism is in the main a US sociological and social psychological perspective that has focused on the reciprocal relationship between language, identity and society. Philosophically it has largely been associated with pragmatists such as James (1907), Mead (1934), Dewey (1922) and Pierce (1958), although in the European context it has affinities with hermeneutics and phenomenology. In addition, it has links with various ‘dramaturgical’ approaches to communication that emphasize the interactive processes underpinning the construction, negotiation, presentation and affirmation of the self. In brief, symbolic interactionism is premised on the supposition that human beings are ‘active’ and not ‘reactive’. Although it is not easy to spell out the central propositions of Symbolic Interactionism in a systematic way, nevertheless, most of its proponents are committed to an interactive view of self and society, that is, they take issue with those views that see the social world as a seamless unity that completely encapsulates and determines individual conduct.