Selecting rhizosphere soil as the object of study which from 5 different kinds of plants in the Inner Mongolia University campus, we use fumigation extraction method and liquid chlorine fumigation extraction-water bath method to determine the microbial biomass carbon in the rhizosphere soil of different plants, in order to compare that whether or not liquid chlorine fumigation extraction-water bath method applicable to this type of soil sample. The results show that, there are significant differences in the rhizosphere soil microbial biomass carbon of 5 different kinds of plants, determination results is that Pyrus sorotina (LS) > Platycladus orientalis (CB) > Prunus persica (TS)> Syringa vulgaris (DX) > Pinus tabuliformis (YS), the content of P. sorotina is highest, 260.53 mg/kg, and P. tabulaeformis is lowest, 121.11 mg/kg, the differ is 139.42 mg/kg. There is a big gap between the measure values of the two methods, so that liquid chlorine fumigation extraction-water bath method doesn’t apply to the determination of this type microbial biomass carbon.
Is microbial biomass measurement by the chloroform fumigation extraction method biased by experimental addition of N and P?
