silver staining technique
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scholarly journals A Study on Keratin-Associated Protein (KAP) 3.2 Gene and Its Polymorphism in Sandyno Breed of Sheep

2020 ◽  
pp. 40-45
Author(s):  
R. Bharathesree ◽  
R. Saravanan ◽  
M. Jeyakumar ◽  
N. Murali
Keyword(s):  
Gene Locus ◽  
Pcr Amplification ◽  
Staining Technique ◽  
Single Strand Conformation Polymorphism ◽  
Effective Number ◽  
Specific Primer ◽  
Hardy Weinberg Equilibrium ◽  
Its Polymorphism ◽  
Silver Staining Technique ◽  
Conformation Polymorphism

The current study investigates the polymorphic patterns of keratin-associated protein (KAP) 3.2 gene in Sandyno breed of sheep. Genomic DNA was isolated from blood samples of 51 numbers of Sandyno breed. Ovine specific primer associated PCR amplification of KAP 3.2 gene revealed product at 393 bp and genotyped by PCR-SSCP (Single Strand Conformation Polymorphism) method and visualized under silver staining technique. KAP 3.2 gene locus revealed 3 genotypes, viz. AA, AB and BB with a frequency of 0.84, 0.16 and 0 in Sandyno breed with allele frequencies of A(0.92) and B(0.08). Regarding population genetic indices, the effective number of alleles (Ne) for KAP 3.2 in Sandyno breed of sheep was found to be 1.1716. The PIC values was 0.1356 and FIS values was negative (– 0.0864) in this breed. The result revealed that the selected population of Sandyno breed of sheep was in Hardy-Weinberg equilibrium without any significant deviation from the population mean and was monomorphic for KAP 3.2 gene.

scholarly journals Utility of a modified silver staining technique for detection of Leptospira

2017 ◽  
Vol 7 (2) ◽  
pp. 85
Author(s):  
C. P. Gunasekara ◽  
M. H. F. Sumaiha ◽  
M. K. S. Damayanthi ◽  
M. M. Weerasekera ◽  
S. S. N. Fernando
Keyword(s):  
Silver Staining ◽  
Staining Technique ◽  
Silver Staining Technique

Demonstration of nucleolar organizer regions in intrahepatic bile duct carcinoma by the silver-staining technique

2008 ◽  
Vol 10 (5) ◽  
pp. 269-277 ◽  
Author(s):  
Akitaka Nonomura ◽  
Fujitsugu Matsubara ◽  
Yuji Mizukami ◽  
Ryohei Izumi ◽  
Yasuni Nakanuma ◽  
...  
Keyword(s):  
Bile Duct ◽  
Silver Staining ◽  
Bile Duct Carcinoma ◽  
Intrahepatic Bile Duct ◽  
Nucleolar Organizer ◽  
Staining Technique ◽  
Nucleolar Organizer Regions ◽  
Duct Carcinoma ◽  
Intrahepatic Bile Duct Carcinoma ◽  
Silver Staining Technique

Identification of nucleolar organizer regions in non-neoplastic and neoplastic hepatocytes by the silver-staining technique

2008 ◽  
Vol 10 (4) ◽  
pp. 229-238 ◽  
Author(s):  
Akitaka Nonomura ◽  
Yuji Mizukami ◽  
Fujitsugu Matsubara ◽  
Yasuni Nakanuma
Keyword(s):  
Silver Staining ◽  
Nucleolar Organizer ◽  
Staining Technique ◽  
Nucleolar Organizer Regions ◽  
Silver Staining Technique

Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique

2003 ◽  
pp. 159-168 ◽  
Author(s):  
Ketan Patel ◽  
David J. Easty ◽  
Michael J. Dunn
Keyword(s):  
Silver Staining ◽  
Staining Technique ◽  
Polyacrylamide Gels ◽  
Silver Staining Technique
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