A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels

2003 ◽  
pp. 73-78
Author(s):  
Jules A. Westbrook ◽  
Michael J. Dunn
Keyword(s):  
Silver Staining ◽  
Staining Technique ◽  
Two Dimensional ◽  
Silver Staining Technique

A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine.

1987 ◽  
Vol 33 (4) ◽  
pp. 561-563 ◽  
Author(s):  
J M Sheat ◽  
M A Lorier
Keyword(s):  
Silver Staining ◽  
Clinical Laboratory ◽  
Staining Technique ◽  
Silver Stain ◽  
Concentration Step ◽  
Silver Staining Technique ◽  
Bence Jones Proteins

Abstract Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.

New approaches to the role of sulfhydryl groups in silver stainability of protein in grasshopper chromosomes

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 133-137 ◽  
Author(s):  
J. de la Torre ◽  
J. L. Bella ◽  
C. López-Fernandez ◽  
J. Gosálvez
Keyword(s):  
Silver Staining ◽  
Chemical Nature ◽  
Germ Line ◽  
Staining Technique ◽  
Sulfhydryl Groups ◽  
Sh Groups ◽  
New Approaches ◽  
Silver Staining Technique ◽  
Reduced State

Silver staining in somatic and germ line cells of the grasshopper Arcyptera fusca has been analyzed after a standard silver staining technique was used with pretreatments that included dithiothreitol and β-mercaptoethanol as reagents to maintain —SH groups in the reduced state. The results show that in some tissues, these pretreatments improve not only the silver staining of nucleolar masses but also the recognition of other silver spots associated with the chromatin. These observations are similar to those previously described for the effect of double-strength standard saline citrate on silver staining. The possible chemical nature of the protein groups responsible for the differential silver stainability and the role of saline citrate in the modification of argyrophylic proteins suggested previously are briefly discussed.Key words: Orthoptera, silver staining, sulfhydryl groups.

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