Methods in Molecular Biology - Cytoskeleton Dynamics
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Published By Springer US

9781071602188, 9781071602195
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scholarly journals Methods for Studying Membrane-Associated Bacterial Cytoskeleton Proteins In Vivo by TIRF Microscopy

2019 ◽  
pp. 123-133 ◽  
Author(s):  
Charlène Cornilleau ◽  
Arnaud Chastanet ◽  
Cyrille Billaudeau ◽  
Rut Carballido-López
Keyword(s):  
Tirf Microscopy ◽  
Bacterial Cytoskeleton

scholarly journals Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes

2019 ◽  
pp. 267-295 ◽  
Author(s):  
Binyam Mogessie
Keyword(s):  
Functional Analysis ◽  
High Resolution ◽  
Chromosome Segregation ◽  
Actin Filaments ◽  
Immunofluorescence Microscopy ◽  
Super Resolution ◽  
Oocyte Meiosis ◽  
Meiotic Spindles

Abstract Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.

scholarly journals Detection of Microtubule Nucleation Hotspots at the Golgi

2019 ◽  
pp. 179-189
Author(s):  
Roslin J. Thoppil ◽  
Anna A. W. M. Sanders ◽  
Irina Kaverina
Keyword(s):  
Microtubule Nucleation

scholarly journals Optogenetic Control of Microtubule Dynamics

2019 ◽  
pp. 211-234
Author(s):  
Jeffrey van Haren ◽  
Lauren S. Adachi ◽  
Torsten Wittmann
Keyword(s):  
Microtubule Dynamics ◽  
Optogenetic Control

In Vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Filaments in Emulsion Droplets

2019 ◽  
pp. 53-75 ◽  
Author(s):  
Kim J. A. Vendel ◽  
Celine Alkemade ◽  
Nemo Andrea ◽  
Gijsje H. Koenderink ◽  
Marileen Dogterom
Keyword(s):  
Actin Filaments ◽  
Emulsion Droplets ◽  
In Vitro Reconstitution

Ultrastructural Analysis of Microtubule Ends

2019 ◽  
pp. 191-209
Author(s):  
J. Richard McIntosh ◽  
Eileen O’Toole ◽  
Cynthia Page ◽  
Garry Morgan
Keyword(s):  
Ultrastructural Analysis

Assaying Microtubule Nucleation

2019 ◽  
pp. 163-178
Author(s):  
Artur Ezquerra ◽  
Ricardo Viais ◽  
Jens Lüders
Keyword(s):  
Microtubule Nucleation
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