DIAGNOSTIC VALUE OF SKIN TESTING

1955 ◽  
Vol 157 (10) ◽  
pp. 825
Keyword(s):  
Skin Testing ◽  
Diagnostic Value

The Importance of Early Erythematous Reaction in Purified Protein Derivative Skin Testing

1994 ◽  
Vol 24 (4) ◽  
pp. 158-160
Author(s):  
I B Barlan ◽  
M M Basaran
Keyword(s):  
Pulmonary Tuberculosis ◽  
Skin Testing ◽  
Diagnostic Value ◽  
Active Tuberculosis ◽  
Purified Protein Derivative

We attempted to assess the diagnostic value of the early erythematous reaction observed at the sixth hour after the application of purified protein derivative (PPD) skin testing. For this purpose, 64 children with pulmonary tuberculosis and 49 healthy age-matched controls were PPD skin tested. Our results showed that the erythematous reaction of 5 mm or greater at the sixth hour was able to detect patients with active tuberculosis with 76% sensitivity, 85% specificity, 87% positive predictivity and 73% negative predictivity. Among 113 subjects, 6 h erythematous reaction of 5 mm or greater in size had 83% sensitivity to detect the ones who subsequently developed 10 mm or greater induration reaction at 48 h. We concluded that the sixth hour early erythematous reaction is just as helpful as the 48 h induration of 10 mm or greater in detecting patients with pulmonary tuberculosis.

scholarly journals Use of Mycobacterium tuberculosisComplex-Specific Antigen Cocktails for a Skin Test Specific for Tuberculosis

1998 ◽  
Vol 66 (8) ◽  
pp. 3606-3610 ◽  
Author(s):  
Konstantin Lyashchenko ◽  
Claudia Manca ◽  
Roberto Colangeli ◽  
Anna Heijbel ◽  
Alan Williams ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Guinea Pigs ◽  
Nontuberculous Mycobacteria ◽  
Skin Testing ◽  
Specific Antigen ◽  
Diagnostic Value ◽  
Mycobacterial Species ◽  
Diagnostic Specificity ◽  
Single Antigen

ABSTRACT The tuberculin skin test currently used to diagnose infection withMycobacterium tuberculosis has poor diagnostic value, especially in geographic areas where the prevalence of tuberculosis is low or where the environmental burden of saprophytic, nontuberculous mycobacteria is high. Inaccuracy of the tuberculin skin test often reflects a low diagnostic specificity due to the presence in tuberculin of antigens shared by many mycobacterial species. Thus, a skin test specific for tuberculosis requires the development of new tuberculins consisting of antigens specific to M. tuberculosis. We have formulated cocktails of two to eight antigens of M. tuberculosis purified from recombinant Escherichia coli. Multiantigen cocktails were evaluated by skin testing guinea pigs sensitized with M. bovis BCG. Reactivity of multiantigen cocktails was greater than that of any single antigen. Cocktail activity increased with the number of antigens in the cocktail even when the same amount of total protein was used for cocktails and for each single antigen. A cocktail of four purified antigens specific for the M. tuberculosis complex elicited skin test responses only in BCG-immunized guinea pigs, not in control animals immunized with M. avium. These findings open the way to designing a multiantigen formulation for a skin test specific for tuberculosis.

Non-immediate reactions to β-lactams: diagnostic value of skin testing and drug provocation test

2008 ◽  
Vol 38 (5) ◽  
pp. 822-828 ◽  
Author(s):  
A. Padial ◽  
C. Antunez ◽  
N. Blanca-Lopez ◽  
T. D. Fernandez ◽  
J. A. Cornejo-Garcia ◽  
...  
Keyword(s):  
Skin Testing ◽  
Provocation Test ◽  
Diagnostic Value ◽  
Drug Provocation Test ◽  
Immediate Reactions

Diagnostic value of recombinant allergen I/a for skin testing and serology

1994 ◽  
Vol 93 (1) ◽  
pp. 1-11 ◽  
Author(s):  
M MOSER ◽  
R CRAMERI ◽  
E BRUST ◽  
M SUTER ◽  
G MENZ
Keyword(s):  
Skin Testing ◽  
Diagnostic Value ◽  
Recombinant Allergen

MILK ALLERGY

PEDIATRICS ◽  
1963 ◽  
Vol 32 (4) ◽  
pp. 572-579
Author(s):  
A. S. Goldman ◽  
W. A. Sellars ◽  
S. R. Halpern ◽  
D. W. Anderson ◽  
T. E. Furlow ◽  
...  
Keyword(s):  
Milk Protein ◽  
Skin Testing ◽  
Protein A ◽  
Milk Proteins ◽  
Close Correlation ◽  
Diagnostic Value ◽  
Skin Tests ◽  
Normal Children ◽  
Diagnostic Significance ◽  
Positive Skin

Skin testing separately and with equal concentrations of the purified milk proteins, casein, alpha-lactalbumin, beta-lactoglobulin, and BSA was done in 85 milk allergic children. For control purposes, intradermal tests with the same antigens were done in 102 normal children and 31 children allergic to non-milk substances. Six per cent of the normal children had weakly positive reactions. However, positive skin tests occurred in 68% of the children allergic, but not to milk, and in 59% of the children allergic to milk. In the milk allergic children, the incidence of positive reactions to each milk protein was similar. The incidence of positive skin tests in milk allergic children was higher than previously apprised. Positive skin tests in the milk allergic child were obtained long after the dietary elimination of milk antigens and after the disappearance of circulating milk antibodies as detected by a variety of techniques. In contrast to the control groups, strongly positive skin tests occurred only in milk allergic patients. Although no correlation was found between the results of all skin tests and the results of oral challenges with the same milk protein, a close correlation was found between the results of strongly positive skin tests and the results of oral challenge with the same milk protein. It appears that weakly positive skin tests with purified milk proteins have a little diagnostic value, but strongly positive skin tests have diagnostic significance.

scholarly journals Comparison of the diagnostic value of two assays for specific immunoglobulin E in suspected beta lactam allergy

2017 ◽  
Vol 70 (7-8) ◽  
pp. 209-215 ◽  
Author(s):  
Mitja Kosnik ◽  
Mira Silar ◽  
Mihaela Zidarn ◽  
Petar Korosec
Keyword(s):  
Immunoglobulin E ◽  
Skin Testing ◽  
Diagnostic Value ◽  
Provocation Tests ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Penicillin Treatment ◽  
Provocation Testing ◽  
Drug Provocation Tests ◽  
Total Immunoglobulin

Introduction. The aim of this study was to compare the diagnostic value of two imunoglobulin E assays for penicillin in a group of patients with a history compatible with hypersensitivity reaction during penicillin treatment who exhibited positive skin testing and/or a drug provocation testing. Material and Methods. In the first part of the study, penicillin G, V and/or amoxicillin specific immunoglobulin E, positive with ImmunoCAP system (Termo Fisher, Waltham, Massachusetts, USA), were selected from our biobank sera, and measured specific immunoglobulin E with Immulite system (Siemens, Munich, Germany). The second part of the study included skin testing, and if negative, drug provocation testing was done with the culprit penicillin in patients with a history compatible with hyper?sensitivity reaction during penicillin treatment. To check the impact of high values of total immunoglobulin E on performance of specific immunoglobulin E, we examined the specific immunoglobulin E to penicillin using both methods in sera of penicillin tolerant patients with total immunoglobulin E over 700 kIU/L. Results. Out of 2.521 in vitro specific immunoglobulin E penicillin tests, 58 were positive with ImmunoCAP. Of these tests, 52 were tested by the Immulite and they were all negative. Among 114 patients with a history compatible with hypersensitivity reac?tion during penicillin treatment, hypersensitivity was confirmed in 36 (31.6%). In 11 patients with positive immediate skin or drug provocation tests, ImmunoCAP was positive in 3 patients and Immulite in 1 patient. Sensitivity of ImmunoCAP and Immulite were 27.1% and 9.1%, respec?tively. Specificity of ImmunoCAP and Immulite were 96.1% and 100%, respectively. Specific immunoglobulin E to penicillins was detected with ImmunoCAP in 8/22 sera with total immunoglobulin E over 700 kIU/L. All sera were negative by the Immulite. Conclusion. Both the Immuno?CAP and Immulite specific immunoglobulin E assays have low sensitiv?ity when assessing patients with a history of immediate hypersensitivity to penicillin, positive immediate skin, or drug provocation tests.

Skin testing with recombinant allergens rBet v 1 and birch profilin, rBet v 2: Diagnostic value for birch pollen and associated allergies

1996 ◽  
Vol 97 (5) ◽  
pp. 1100-1109 ◽  
Author(s):  
Gabrielle Pauli ◽  
Jean Philippe Oster ◽  
Philippe Deviller ◽  
Suzanne Heiss ◽  
Jean Claude Bessot ◽  
...  
Keyword(s):  
Skin Testing ◽  
Birch Pollen ◽  
Diagnostic Value ◽  
Recombinant Allergens

Diagnostic Value of Electron Microscopy in Soft Tissue Tumors

1970 ◽  
Vol 28 ◽  
pp. 220-221
Author(s):  
Gerald Fine ◽  
Azorides R. Morales
Keyword(s):  
Cardiac Myxoma ◽  
Osteogenic Sarcoma ◽  
Diagnostic Value ◽  
Soft Tissue Tumors ◽  
Amelanotic Melanoma ◽  
Growth Patterns ◽  
Light Microscopic Level ◽  
Mesenchymal Tumors ◽  
Good Agreement

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)

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