Background:
Compared to polymeric nanoparticles prepared using non-lipid surfactants,
lecithin addition forms larger nanoparticles and exhibits higher drug loading and the stability of
nanoparticles can be conferred by adding Vitamin E Polyethylene Glycol Succinate (TPGS) into the
formulation.
Aim:
The aim of this study is to prepare Gemcitabine (Gem) loaded lecithin/PLGA nanoparticles.
Moreover, the effect of TPGS and sodium cholate (SK) on the preparation of lecithin/PLGA nanoparticles
was compared.
Methods:
It was found that while PC addition into PLGATPGS nanoparticles formed larger particles
(251.3± 6.0 nm for Gem-PLGATPGS NPs and 516,9 ± 3.9 nm for Gem-PLGA-PCTPGS NPs), the particle
size of PLGASK nanoparticles was not affected by lecithin addition (p>0.05).
Results:
In cytotoxicity studies, it was found that the SK-MES-1 cell inhibition rates of Gem-PLGATPGS
NPs, Gem-PLGA-PCTPGS NPs, Gem-PLGASK NPs, Gem-PLGA-PCSK NPs were similar with free Gem
(p>0.05). In cytotoxicity studies, it was found that the encapsulation into nanoparticles did not change
the cytotoxicity of the drug. However, higher cellular uptake has been observed when the lecithin was
used in the preparation of PLGA nanoparticles.
Conclusion:
Compared with free Gem, the Gem-loaded nanoparticles enhanced the uptake of the drug
by SK-MES-1 cells which can increase the effect of gemcitabine for non-small cell lung cancer therapy.
Present status and upcoming prospects of hedgehog pathway inhibitors in small cell lung cancer therapy
