scholarly journals Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase fromJonesia denitrificans

FEBS Letters ◽  
2015 ◽  
Vol 590 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Sophanit Mekasha ◽  
Zarah Forsberg ◽  
Bjørn Dalhus ◽  
John-Paul Bacik ◽  
Swati Choudhary ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

scholarly journals Structural and Functional Characterization of a Lytic Polysaccharide Monooxygenase with Broad Substrate Specificity

2015 ◽  
Vol 290 (38) ◽  
pp. 22955-22969 ◽  
Author(s):  
Anna S. Borisova ◽  
Trine Isaksen ◽  
Maria Dimarogona ◽  
Abhishek A. Kognole ◽  
Geir Mathiesen ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Functional Characterization ◽  
Lytic Polysaccharide Monooxygenase ◽  
Broad Substrate Specificity ◽  
Polysaccharide Monooxygenase

scholarly journals Functional characterization of a lytic polysaccharide monooxygenase from the thermophilic fungus Myceliophthora thermophila

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202148 ◽  
Author(s):  
Marco A. S. Kadowaki ◽  
Anikó Várnai ◽  
John-Kristian Jameson ◽  
Ana E. T. Leite ◽  
Antonio J. Costa-Filho ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Thermophilic Fungus ◽  
Myceliophthora Thermophila ◽  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

Identification and characterization of a novel AA9-type lytic polysaccharide monooxygenase from a bagasse metagenome

2020 ◽  
Vol 105 (1) ◽  
pp. 197-210
Author(s):  
Benjarat Bunterngsook ◽  
Wuttichai Mhuantong ◽  
Pattanop Kanokratana ◽  
Yu Iseki ◽  
Takashi Watanabe ◽  
...  
Keyword(s):  
Lytic Polysaccharide Monooxygenase ◽  
Identification And Characterization ◽  
Polysaccharide Monooxygenase

scholarly journals Characterization of a lytic polysaccharide monooxygenase from Aspergillus fumigatus shows functional variation among family AA11 fungal LPMOs

2021 ◽  
pp. 101421
Author(s):  
Fredrik Gjerstad Støpamo ◽  
Åsmund Kjendseth Røhr ◽  
Sophanit Mekasha ◽  
Dejan M. Petrović ◽  
Anikó Várnai ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Functional Variation ◽  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

scholarly journals Genomic and transcriptomic analysis of the thermophilic lignocellulose-degrading fungus Thielavia terrestris LPH172

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Monika Tõlgo ◽  
Silvia Hüttner ◽  
Peter Rugbjerg ◽  
Nguyen Thanh Thuy ◽  
Vu Nguyen Thanh ◽  
...  
Keyword(s):  
Gc Content ◽  
Carbohydrate Active Enzymes ◽  
Lytic Polysaccharide Monooxygenase ◽  
Thielavia Terrestris ◽  
Biomass Saccharification ◽  
Beechwood Xylan ◽  
Genes Encoding ◽  
Encoding Genes ◽  
Polysaccharide Monooxygenase

Abstract Background Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification and make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active enzymes (CAZymes) for biomass degradation. The newly isolated LPH172 strain of the thermophilic Ascomycete Thielavia terrestris has been shown to possess high xylanase and cellulase activities and tolerate low pH and high temperatures. Here, we aimed to illuminate the lignocellulose-degrading machinery and novel carbohydrate-active enzymes in LPH172 in detail. Results We sequenced and analyzed the 36.6-Mb genome and transcriptome of LPH172 during growth on glucose, cellulose, rice straw, and beechwood xylan. 10,128 predicted genes were found in total, which included 411 CAZy domains. Compared to other fungi, auxiliary activity (AA) domains were particularly enriched. A higher GC content was found in coding sequences compared to the overall genome, as well as a high GC3 content, which is hypothesized to contribute to thermophilicity. Primarily auxiliary activity (AA) family 9 lytic polysaccharide monooxygenase (LPMO) and glycoside hydrolase (GH) family 7 glucanase encoding genes were upregulated when LPH172 was cultivated on cellulosic substrates. Conventional hemicellulose encoding genes (GH10, GH11 and various CEs), as well as AA9 LPMOs, were upregulated when LPH172 was cultivated on xylan. The observed co-expression and co-upregulation of genes encoding AA9 LPMOs, other AA CAZymes, and (hemi)cellulases point to a complex and nuanced degradation strategy. Conclusions Our analysis of the genome and transcriptome of T. terrestris LPH172 elucidates the enzyme arsenal that the fungus uses to degrade lignocellulosic substrates. The study provides the basis for future characterization of potential new enzymes for industrial biomass saccharification.

scholarly journals Characterization of a bacterial copper‐dependent lytic polysaccharide monooxygenase with an unusual second coordination sphere

FEBS Journal ◽  
2020 ◽  
Vol 287 (15) ◽  
pp. 3298-3314 ◽  
Author(s):  
Alessia Munzone ◽  
Bilal El Kerdi ◽  
Mathieu Fanuel ◽  
Hélène Rogniaux ◽  
David Ropartz ◽  
...  
Keyword(s):  
Coordination Sphere ◽  
Lytic Polysaccharide Monooxygenase ◽  
Second Coordination Sphere ◽  
Polysaccharide Monooxygenase

Purification and characterization of two forms of the homologously expressed lytic polysaccharide monooxygenase (PvLPMO9A) from Penicillium verruculosum

2020 ◽  
Vol 1868 (1) ◽  
pp. 140297 ◽  
Author(s):  
Margarita V. Semenova ◽  
Alexander V. Gusakov ◽  
Vadim D. Telitsin ◽  
Aleksandra M. Rozhkova ◽  
Elena G. Kondratyeva ◽  
...  
Keyword(s):  
Penicillium Verruculosum ◽  
Purification And Characterization ◽  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

scholarly journals Detection and Characterization of a Novel Copper‐Dependent Intermediate in a Lytic Polysaccharide Monooxygenase

2019 ◽  
Vol 26 (2) ◽  
pp. 454-463 ◽  
Author(s):  
Raushan K. Singh ◽  
Benedikt M. Blossom ◽  
David A. Russo ◽  
Ranjitha Singh ◽  
Høgni Weihe ◽  
...  
Keyword(s):  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity

2020 ◽  
Vol 12 (2) ◽  
pp. 149-161 ◽  
Author(s):  
Caio de Oliveira Gorgulho Silva ◽  
Tallyta Santos Teixeira ◽  
Kelly Barreto Rodrigues ◽  
Amanda Araújo Souza ◽  
Antonielle Vieira Monclaro ◽  
...  
Keyword(s):  
Lytic Polysaccharide Monooxygenase ◽  
Monooxygenase Activity ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Lytic Polysaccharide Monooxygenases ◽  
Polysaccharide Monooxygenases ◽  
Tof Ms ◽  
Polysaccharide Monooxygenase

Two new mass spectrometry methods, MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS, were developed for the characterization of cellulose-active lytic polysaccharide monooxygenases, expanding the analytical toolbox for the study of these enzymes.

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