Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity

Two new mass spectrometry methods, MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS, were developed for the characterization of cellulose-active lytic polysaccharide monooxygenases, expanding the analytical toolbox for the study of these enzymes.

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Characterization of polyethers using different methods: SFC, LAC and SEC with different detectors, and MALDI-TOF-MS

Macromolecular Symposia ◽

10.1002/masy.19961100117 ◽

1996 ◽

Vol 110 (1) ◽

pp. 231-240 ◽

Cited By ~ 17

Author(s):

Bernd Trathnigg ◽

Bernhard Maier ◽

Günther Schulz ◽

Ralph-Peter Krüger ◽

Ulrich Just

Keyword(s):

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

Journal of Agricultural and Food Chemistry ◽

10.1021/jf300740d ◽

2012 ◽

Vol 60 (19) ◽

pp. 5013-5022 ◽

Cited By ~ 53

Author(s):

Wei-Ming Chai ◽

Yan Shi ◽

Hui-Ling Feng ◽

Ling Qiu ◽

Hai-Chao Zhou ◽

...

Keyword(s):

Condensed Tannins ◽

Maldi Tof Ms ◽

Esi Ms ◽

Delonix Regia ◽

Maldi Tof ◽

Ms Analysis ◽

Tof Ms

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Multidimensional Characterization of α,ω-Telechelic Poly(ε-caprolactone)s via Online Coupling of 2D Chromatographic Methods (LC/SEC) and ESI-TOF/MALDI-TOF-MS

Macromolecules ◽

10.1021/ma3016739 ◽

2012 ◽

Vol 45 (24) ◽

pp. 9779-9790 ◽

Cited By ~ 20

Author(s):

Haitham Barqawi ◽

Elena Ostas ◽

Bo Liu ◽

Jean-François Carpentier ◽

Wolfgang H. Binder

Keyword(s):

Maldi Tof Ms ◽

Chromatographic Methods ◽

Maldi Tof ◽

Tof Ms

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Characterization of pathogenic bacteria using ionic liquid via single drop microextraction combined with MALDI-TOF MS

The Analyst ◽

10.1039/c1an15350a ◽

2011 ◽

Vol 136 (19) ◽

pp. 4020 ◽

Cited By ~ 27

Author(s):

Faheem Ahmad ◽

Hui-Fen Wu

Keyword(s):

Ionic Liquid ◽

Pathogenic Bacteria ◽

Single Drop ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Single Drop Microextraction ◽

Tof Ms

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Synthesis and characterization of polycarbodiimides by MALDI-TOF MS and NMR spectroscopy: kinetic and mechanism study

Polymer Bulletin ◽

10.1007/s00289-017-2175-z ◽

2017 ◽

Vol 75 (6) ◽

pp. 2657-2670

Author(s):

Albert Morell Garcia ◽

Francisco Medina Cabello ◽

Antonio Torrelles Nogués

Keyword(s):

Nmr Spectroscopy ◽

Synthesis And Characterization ◽

Mechanism Study ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Recent Advances in Screening Methods for the Functional Investigation of Lytic Polysaccharide Monooxygenases

Frontiers in Chemistry ◽

10.3389/fchem.2021.653754 ◽

2021 ◽

Vol 9 ◽

Author(s):

Damao Wang ◽

Yanping Li ◽

Yuting Zheng ◽

Yves S. Y. Hsieh

Keyword(s):

Copper Ions ◽

Biomass Conversion ◽

Screening Methods ◽

Lytic Polysaccharide Monooxygenase ◽

Indirect Measurements ◽

Glycosidic Bonds ◽

Lytic Polysaccharide Monooxygenases ◽

Polysaccharide Monooxygenases ◽

Functional Investigation ◽

Polysaccharide Monooxygenase

Lytic polysaccharide monooxygenase (LPMO) is a newly discovered and widely studied enzyme in recent years. These enzymes play a key role in the depolymerization of sugar-based biopolymers (including cellulose, hemicellulose, chitin and starch), and have a positive significance for biomass conversion. LPMO is a copper-dependent enzyme that can oxidize and cleave glycosidic bonds in cellulose and other polysaccharides. Their mechanism of action depends on the correct coordination of copper ions in the active site. There are still difficulties in the analysis of LPMO activity, which often requires multiple methods to be used in concert. In this review, we discussed various LPMO activity analysis methods reported so far, including mature mass spectrometry, chromatography, labeling, and indirect measurements, and summarized the advantages, disadvantages and applicability of different methods.

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