myceliophthora thermophila
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2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jingen Li ◽  
Bingchen Chen ◽  
Shuying Gu ◽  
Zhen Zhao ◽  
Qian Liu ◽  
...  

Abstract Background Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO2 into carbon metabolism for increased carbon efficiency in producing value-added compounds is another strategy for cost-effective bio-manufacturing. It has not been reported whether these two strategies can be combined in one microbial platform. Results In this study, using the cellulolytic thermophilic fungus Myceliophthora thermophila, we designed and constructed a novel biorefinery system DMCC (Direct microbial conversion of biomass with CO2 fixation) through incorporating two CO2 fixation modules, PYC module and Calvin–Benson–Bassham (CBB) pathway. Harboring the both modules, the average rate of fixing and channeling 13CO2 into malic acid in strain CP51 achieved 44.4, 90.7, and 80.7 mg/L/h, on xylose, glucose, and cellulose, respectively. The corresponding titers of malic acid were up to 42.1, 70.4, and 70.1 g/L, respectively, representing the increases of 40%, 10%, and 7%, respectively, compared to the parental strain possessing only PYC module. The DMCC system was further improved by enhancing the pentose uptake ability. Using raw plant biomass as the feedstock, yield of malic acid produced by the DMCC system was up to 0.53 g/g, with 13C content of 0.44 mol/mol malic acid, suggesting DMCC system can produce 1 t of malic acid from 1.89 t of biomass and fix 0.14 t CO2 accordingly. Conclusions This study designed and constructed a novel biorefinery system named DMCC, which can convert raw plant biomass and CO2 into organic acid efficiently, presenting a promising strategy for cost-effective production of value-added compounds in biorefinery. The DMCC system is one of great options for realization of carbon neutral economy.


Author(s):  
Jou Chin Chan ◽  
Bixia Zhang ◽  
Michael Martinez ◽  
Balaganesh Kuruba ◽  
James Brozik ◽  
...  

2021 ◽  
Vol 12 ◽  
Author(s):  
Hanyu Wang ◽  
Tao Sun ◽  
Zhen Zhao ◽  
Shuying Gu ◽  
Qian Liu ◽  
...  

Efficient biological conversion of all sugars from lignocellulosic biomass is necessary for the cost-effective production of biofuels and commodity chemicals. Galactose is one of the most abundant sugar in many hemicelluloses, and it will be important to capture this carbon for an efficient bioconversion process of plant biomass. Thermophilic fungus Myceliophthora thermophila has been used as a cell factory to produce biochemicals directly from renewable polysaccharides. In this study, we draw out the two native galactose utilization pathways, including the Leloir pathway and oxido-reductive pathway, and identify the significance and contribution of them, through transcriptional profiling analysis of M. thermophila and its mutants on galactose. We find that galactokinase was necessary for galactose transporter expression, and disruption of galK resulted in decreased galactose utilization. Through metabolic engineering, both galactokinase deletion and galactose transporter overexpression can activate internal the oxido-reductive pathway and improve the consumption rate of galactose. Finally, the heterologous galactose-degradation pathway, De Ley–Doudoroff (DLD) pathway, was successfully integrated into M. thermophila, and the consumption rate of galactose in the engineered strain was increased by 57%. Our study focuses on metabolic engineering for accelerating galactose utilization in a thermophilic fungus that will be beneficial for the rational design of fungal strains to produce biofuels and biochemicals from a variety of feedstocks with abundant galactose.


2020 ◽  
Vol 61 ◽  
pp. 416-426 ◽  
Author(s):  
Jingen Li ◽  
Liangcai Lin ◽  
Tao Sun ◽  
Jing Xu ◽  
Jingxiao Ji ◽  
...  

Author(s):  
Maria Angela B. Grieco ◽  
Mireille Haon ◽  
Sacha Grisel ◽  
Ana Lucia de Oliveira-Carvalho ◽  
Augusto Vieira Magalhães ◽  
...  

2020 ◽  
Vol 27 ◽  
pp. 101684
Author(s):  
Vanessa Salto Massarente ◽  
Jéssica de Araujo Zanoni ◽  
Eleni Gomes ◽  
Gustavo Orlando Bonilla-Rodriguez

2020 ◽  
Vol 130 (1) ◽  
pp. 90-99
Author(s):  
A.C. Santos Gomes ◽  
F.P. Casciatori ◽  
E. Gomes ◽  
C. Costa Carreira Nunes ◽  
M.M.S. Moretti ◽  
...  

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