scholarly journals Microelectrode Arrays: Electrophysiological Phenotype Characterization of Human iPSC‐Derived Neuronal Cell Lines by Means of High‐Density Microelectrode Arrays (Adv. Biology 3/2021)

2021 ◽  
Vol 5 (3) ◽  
pp. 2170031
Author(s):  
Silvia Ronchi ◽  
Alessio Paolo Buccino ◽  
Gustavo Prack ◽  
Sreedhar Saseendran Kumar ◽  
Manuel Schröter ◽  
...  
Keyword(s):  
Cell Lines ◽  
Microelectrode Arrays ◽  
Neuronal Cell ◽  
High Density ◽  
Phenotype Characterization ◽  
Neuronal Cell Lines ◽  
Human Ipsc

scholarly journals Electrophysiological Phenotype Characterization of Human iPSC‐Derived Neuronal Cell Lines by Means of High‐Density Microelectrode Arrays

2021 ◽  
pp. 2000223
Author(s):  
Silvia Ronchi ◽  
Alessio Paolo Buccino ◽  
Gustavo Prack ◽  
Sreedhar Saseendran Kumar ◽  
Manuel Schröter ◽  
...  
Keyword(s):  
Cell Lines ◽  
Microelectrode Arrays ◽  
Neuronal Cell ◽  
High Density ◽  
Phenotype Characterization ◽  
Neuronal Cell Lines ◽  
Human Ipsc

scholarly journals Characterization of Fetal and Postnatal Enteric Neuronal Cell Lines With Improvement in Intestinal Neural Function

2008 ◽  
Vol 134 (5) ◽  
pp. 1424-1435 ◽  
Author(s):  
Mallappa Anitha ◽  
Irene Joseph ◽  
Xiaokun Ding ◽  
Enrique R. Torre ◽  
Michael A. Sawchuk ◽  
...  
Keyword(s):  
Cell Lines ◽  
Neuronal Cell ◽  
Neural Function ◽  
Neuronal Cell Lines

Establishment and characterization of immortalized neuronal cell lines derived from the spinal cord of normal and trisomy 16 fetal mice, an animal model of Down syndrome

2002 ◽  
Vol 68 (1) ◽  
pp. 46-58 ◽  
Author(s):  
Ana María Cárdenas ◽  
David D. Allen ◽  
Christian Arriagada ◽  
Alexis Olivares ◽  
Lori B. Bennett ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Animal Model ◽  
Down Syndrome ◽  
Cell Lines ◽  
Neuronal Cell ◽  
Trisomy 16 ◽  
Fetal Mice ◽  
Neuronal Cell Lines

Characterization of endogenous calcium responses in neuronal cell lines

2010 ◽  
Vol 79 (6) ◽  
pp. 908-920 ◽  
Author(s):  
Irina Vetter ◽  
Richard J. Lewis
Keyword(s):  
Cell Lines ◽  
Neuronal Cell ◽  
Neuronal Cell Lines

The derivation and characterization of neuronal cell lines from rat and mouse brain

1977 ◽  
Vol 135 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Karen Bulloch ◽  
William B. Stallcup ◽  
Melvin Cohn
Keyword(s):  
Cell Lines ◽  
Mouse Brain ◽  
Neuronal Cell ◽  
Neuronal Cell Lines

scholarly journals Electrophysiological Phenotype Characterization of Human iPSC-Derived Neuronal Cell Lines by Means of High-Density Microelectrode Arrays

2020 ◽  
Author(s):  
Silvia Ronchi ◽  
Alessio Paolo Buccino ◽  
Gustavo Prack ◽  
Sreedhar Saseendran Kumar ◽  
Manuel Schröter ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Drug Testing ◽  
Microelectrode Arrays ◽  
Neuronal Cell ◽  
Drug Effects ◽  
Induced Pluripotent Stem Cell ◽  
Cellular Reprogramming ◽  
High Density ◽  
Phenotype Characterization

AbstractRecent advances in the field of cellular reprogramming have opened a route to study the fundamental mechanisms underlying common neurological disorders. High-density microelectrode-arrays (HD-MEAs) provide unprecedented means to study neuronal physiology at different scales, ranging from network through single-neuron to subcellular features. In this work, we used HD-MEAs in vitro to characterize and compare human induced-pluripotent-stem-cell (iPSC)-derived dopaminergic and motor neurons, including isogenic neuronal lines modeling Parkinson’s disease and amyotrophic lateral sclerosis. We established reproducible electrophysiological network, single-cell and subcellular metrics, which were used for phenotype characterization and drug testing. Metrics such as burst shapes and axonal velocity enabled the distinction of healthy and diseased neurons. The HD-MEA metrics could also be used to detect the effects of dosing the drug retigabine to human motor neurons. Finally, we showed that the ability to detect drug effects and the observed culture-to-culture variability critically depend on the number of available recording electrodes.

Sign in / Sign up

Export Citation Format

Share Document