scholarly journals Bone Regeneration: Embryonic‐Like Mineralized Extracellular Matrix/Stem Cell Microspheroids as a Bone Graft Substitute (Adv. Healthcare Mater. 19/2018)

2018 ◽  
Vol 7 (19) ◽  
pp. 1870076
Author(s):  
Cuicui Fu ◽  
Dan Luo ◽  
Min Yu ◽  
Nan Jiang ◽  
Dawei Liu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Graft Substitute

Embryonic‐Like Mineralized Extracellular Matrix/Stem Cell Microspheroids as a Bone Graft Substitute

2018 ◽  
Vol 7 (19) ◽  
pp. 1800705 ◽  
Author(s):  
Cuicui Fu ◽  
Dan Luo ◽  
Min Yu ◽  
Nan Jiang ◽  
Dawei Liu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Bone Graft ◽  
Bone Graft Substitute

scholarly journals Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

2019 ◽  
Vol 20 (16) ◽  
pp. 3970 ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Susumu Horikoshi ◽  
Tomoya Ogawa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Host Cells ◽  
Dependent Manner ◽  
Serum Free ◽  
Serum Free Conditions ◽  
Free Serum

Three-dimensional clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. We demonstrated previously that C-MSCs can be transplanted into bone defect regions with no artificial scaffold to induce bone regeneration. To apply C-MSCs in a clinical setting as a reliable bone regenerative therapy, the present study aimed to generate C-MSCs in xeno-free/serum-free conditions that can exert successful bone regenerative properties and to monitor interactions between grafted cells and host cells during bone healing processes. Human bone marrow-derived MSCs were cultured in xeno-free/serum-free medium. To obtain C-MSCs, confluent cells that had formed on the cellular sheet were scratched using a micropipette tip and then torn off. The sheet was rolled to make a round clump of cells. Then, C-MSCs were transplanted into an immunodeficient mouse calvarial defect model. Transplantation of C-MSCs induced bone regeneration in a time-dependent manner. Immunofluorescence staining showed that both donor human cells and host mice cells contributed to bone reconstruction. Decellularized C-MSCs implantation failed to induce bone regeneration, even though the host mice cells can infiltrate into the defect area. These findings suggested that C-MSCs generated in xeno-free/serum-free conditions can induce bone regeneration via direct and indirect osteogenesis.

Harnessing the Osteogenicity of In Vitro Stem Cell-Derived Mineralized Extracellular Matrix as 3D Biotemplate to Guide Bone Regeneration

2017 ◽  
Vol 23 (17-18) ◽  
pp. 874-890 ◽  
Author(s):  
Yoke Chin Chai ◽  
Johanna Bolander ◽  
Ioannis Papantoniou ◽  
Jennifer Patterson ◽  
Jef Vleugels ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Bone Regeneration ◽  
Guide Bone Regeneration

scholarly journals Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

2015 ◽  
Vol 4 (5) ◽  
pp. 538-538 ◽  
Author(s):  
Choon G. Chung ◽  
Aaron W. James ◽  
Greg Asatrian ◽  
Le Chang ◽  
Alan Nguyen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Graft ◽  
Spinal Fusion ◽  
Bone Graft Substitute

scholarly journals Biosilicated collagen/β-tricalcium phosphate composites as a BMP-2-delivering bone‐graft substitute for accelerated craniofacial bone regeneration

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Dong Keon Lee ◽  
Mi-Ran Ki ◽  
Euy Hyun Kim ◽  
Chang-Joo Park ◽  
Jae Jun Ryu ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Bone Grafting ◽  
Drug Carrier ◽  
Bone Graft Substitute ◽  
Tissue Growth ◽  
Bone Morphogenetic Protein 2 ◽  
Therapeutic Drug

Abstract Background Bioceramic β-tricalcium phosphate (β-TCP) is used as a bone-grafting material and a therapeutic drug carrier for treatment of bone defects in the oral and maxillofacial regions due to the osteoconductivity and biocompatibility. However, the low mechanical strength and limited osteoinductivity of β-TCP agglomerate restrict bone regenerating performance in clinical settings. Methods Herein, a biomimetic composite is proposed as a bone morphogenetic protein-2 (BMP-2)-delivering bone graft substitute to achieve a robust bone grafting and augmented bone regeneration. Results The sequential processes of brown algae-inspired biosilicification and collagen coating on the surface of β-TCP enable the effective incorporation of BMP-2 into the coating layer without losing its bioactivity. The sustained delivery of BMP-2 from the biosilicated collagen and β-TCP composites promoted in vitro osteogenic behaviors of pre-osteoblasts and remarkedly accelerated in vivo bone regeneration within a rat calvarial bone defect. Conclusions Our multicomposite bone substitutes can be practically applied to improve bone tissue growth in bone grafting applications with further expansion to general bone tissue engineering.

scholarly journals Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

2014 ◽  
Vol 3 (10) ◽  
pp. 1231-1241 ◽  
Author(s):  
Choon G. Chung ◽  
Aaron W. James ◽  
Greg Asatrian ◽  
Le Chang ◽  
Alan Nguyen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Graft ◽  
Spinal Fusion ◽  
Bone Graft Substitute

scholarly journals Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Manabu Takewaki ◽  
Susumu Horikoshi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration

Bone regeneration through facile binding of bone graft substitute particles using mussel adhesive protein

2014 ◽  
Vol 31 ◽  
pp. S53
Author(s):  
Hyung Joon Cha ◽  
Bong-Hyuk Choi ◽  
Hogyun Cheong ◽  
Yun Kee Jo ◽  
Jin-Soo Ahn ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Bone Graft Substitute ◽  
Adhesive Protein ◽  
Mussel Adhesive Protein ◽  
Mussel Adhesive
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