scholarly journals Cryopreserved clumps of mesenchymal stem cell/extracellular matrix complexes retain osteogenic capacity and induce bone regeneration

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Manabu Takewaki ◽  
Susumu Horikoshi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration

scholarly journals Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

2019 ◽  
Vol 20 (16) ◽  
pp. 3970 ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Susumu Horikoshi ◽  
Tomoya Ogawa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Host Cells ◽  
Dependent Manner ◽  
Serum Free ◽  
Serum Free Conditions ◽  
Free Serum

Three-dimensional clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. We demonstrated previously that C-MSCs can be transplanted into bone defect regions with no artificial scaffold to induce bone regeneration. To apply C-MSCs in a clinical setting as a reliable bone regenerative therapy, the present study aimed to generate C-MSCs in xeno-free/serum-free conditions that can exert successful bone regenerative properties and to monitor interactions between grafted cells and host cells during bone healing processes. Human bone marrow-derived MSCs were cultured in xeno-free/serum-free medium. To obtain C-MSCs, confluent cells that had formed on the cellular sheet were scratched using a micropipette tip and then torn off. The sheet was rolled to make a round clump of cells. Then, C-MSCs were transplanted into an immunodeficient mouse calvarial defect model. Transplantation of C-MSCs induced bone regeneration in a time-dependent manner. Immunofluorescence staining showed that both donor human cells and host mice cells contributed to bone reconstruction. Decellularized C-MSCs implantation failed to induce bone regeneration, even though the host mice cells can infiltrate into the defect area. These findings suggested that C-MSCs generated in xeno-free/serum-free conditions can induce bone regeneration via direct and indirect osteogenesis.

scholarly journals Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

2020 ◽  
Vol 11 ◽  
pp. 204173142092691 ◽  
Author(s):  
Rui Dong ◽  
Yun Bai ◽  
Jingjin Dai ◽  
Moyuan Deng ◽  
Chunrong Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Complex Biological Process

Recently, extracellular matrix-based tissue-engineered bone is a promising approach to repairing bone defects, and the seed cells are mostly mesenchymal stem cells. However, bone remodelling is a complex biological process, in which osteoclasts perform bone resorption and osteoblasts dominate bone formation. The interaction and coupling of these two kinds of cells is the key to bone repair. Therefore, the extracellular matrix secreted by the mesenchymal stem cells alone cannot mimic a complex bone regeneration microenvironment, and the addition of extracellular matrix by preosteoclasts may contribute as an effective strategy for bone regeneration. Here, we established the mesenchymal stem cell/preosteoclast extracellular matrix -based tissue-engineered bones and demonstrated that engineered-scaffolds based on mesenchymal stem cell/ preosteoclast extracellular matrix significantly enhanced osteogenesis in a 3 mm rat femur defect model compared with mesenchymal stem cell alone. The bioactive proteins released from the mesenchymal stem cell/ preosteoclast extracellular matrix based tissue-engineered bones also promoted the migration, adhesion, and osteogenic differentiation of mesenchymal stem cells in vitro. As for the mechanisms, the iTRAQ-labeled mass spectrometry was performed, and 608 differentially expressed proteins were found, including the IGFBP5 and CXCL12. Through in vitro studies, we proved that CXCL12 and IGFBP5 proteins, mainly released from the preosteoclasts, contributed to mesenchymal stem cells migration and osteogenic differentiation, respectively. Overall, our research, for the first time, introduce pre-osteoclast into the tissue engineering of bone and optimize the strategy of constructing extracellular matrix–based tissue-engineered bone using different cells to simulate the natural bone regeneration environment, which provides new sight for bone tissue engineering.

A cancellous bone matrix system with specific mineralisation degrees for mesenchymal stem cell differentiation and bone regeneration

2019 ◽  
Vol 7 (6) ◽  
pp. 2452-2467 ◽  
Author(s):  
Shijie Liu ◽  
Yiyun Wang ◽  
Jian Wang ◽  
Pengcheng Qiu ◽  
Shengyu Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Bone Matrix ◽  
Stem Cell Differentiation ◽  
Matrix System ◽  
Mesenchymal Stem Cell Differentiation ◽  
Regenerative Therapies

Bone regenerative therapies have been explored using various biomaterial systems.

scholarly journals IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4+ T cells

2016 ◽  
Vol 23 (12) ◽  
pp. 1941-1951 ◽  
Author(s):  
Jiajia Xu ◽  
Yiyun Wang ◽  
Jing Li ◽  
Xudong Zhang ◽  
Yiyun Geng ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Cd4 T Cells

scholarly journals Mesenchymal Stem Cell Seeding of Porcine Small Intestinal Submucosal Extracellular Matrix for Cardiovascular Applications

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153412 ◽  
Author(s):  
Chia Wei Chang ◽  
Tye Petrie ◽  
Alycia Clark ◽  
Xin Lin ◽  
Claus S. Sondergaard ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Seeding ◽  
Small Intestinal
Sign in / Sign up

Export Citation Format

Share Document