scholarly journals Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy

2016 ◽  
Vol 128 (39) ◽  
pp. 12187-12191 ◽  
Author(s):  
Michael Andreas Juen ◽  
Christoph Hermann Wunderlich ◽  
Felix Nußbaumer ◽  
Martin Tollinger ◽  
Georg Kontaxis ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Nucleic Acids ◽  
Excited States ◽  
Relaxation Dispersion ◽  
Proton Relaxation ◽  
Relaxation Dispersion Nmr

scholarly journals Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy

2016 ◽  
Vol 55 (39) ◽  
pp. 12008-12012 ◽  
Author(s):  
Michael Andreas Juen ◽  
Christoph Hermann Wunderlich ◽  
Felix Nußbaumer ◽  
Martin Tollinger ◽  
Georg Kontaxis ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Nucleic Acids ◽  
Excited States ◽  
Relaxation Dispersion ◽  
Proton Relaxation ◽  
Relaxation Dispersion Nmr

Measurement of Methyl Axis Orientations in Invisible, Excited States of Proteins by Relaxation Dispersion NMR Spectroscopy

2009 ◽  
Vol 131 (33) ◽  
pp. 11939-11948 ◽  
Author(s):  
Andrew J. Baldwin ◽  
D. Flemming Hansen ◽  
Pramodh Vallurupalli ◽  
Lewis E. Kay
Keyword(s):  
Nmr Spectroscopy ◽  
Excited States ◽  
Relaxation Dispersion ◽  
Relaxation Dispersion Nmr ◽  
Invisible Excited States

scholarly journals How internal cavities destabilize a protein

2019 ◽  
Vol 116 (42) ◽  
pp. 21031-21036 ◽  
Author(s):  
Mengjun Xue ◽  
Takuro Wakamoto ◽  
Camilla Kejlberg ◽  
Yuichi Yoshimura ◽  
Tania Aaquist Nielsen ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Molar Volume ◽  
Excited States ◽  
Partial Molar Volume ◽  
Protein Misfolding ◽  
Structural Information ◽  
Relaxation Dispersion ◽  
Internal Cavity ◽  
Cavity Volume ◽  
Internal Cavities

Although many proteins possess a distinct folded structure lying at a minimum in a funneled free energy landscape, thermal energy causes any protein to continuously access lowly populated excited states. The existence of excited states is an integral part of biological function. Although transitions into the excited states may lead to protein misfolding and aggregation, little structural information is currently available for them. Here, we show how NMR spectroscopy, coupled with pressure perturbation, brings these elusive species to light. As pressure acts to favor states with lower partial molar volume, NMR follows the ensuing change in the equilibrium spectroscopically, with residue-specific resolution. For T4 lysozyme L99A, relaxation dispersion NMR was used to follow the increase in population of a previously identified “invisible” folded state with pressure, as this is driven by the reduction in cavity volume by the flipping-in of a surface aromatic group. Furthermore, multiple partly disordered excited states were detected at equilibrium using pressure-dependent H/D exchange NMR spectroscopy. Here, unfolding reduced partial molar volume by the removal of empty internal cavities and packing imperfections through subglobal and global unfolding. A close correspondence was found for the distinct pressure sensitivities of various parts of the protein and the amount of internal cavity volume that was lost in each unfolding event. The free energies and populations of excited states allowed us to determine the energetic penalty of empty internal protein cavities to be 36 cal⋅Å−3.

scholarly journals Probing RNA dynamics via longitudinal exchange and CPMG relaxation dispersion NMR spectroscopy using a sensitive 13C-methyl label

2011 ◽  
Vol 39 (10) ◽  
pp. 4340-4351 ◽  
Author(s):  
Karin Kloiber ◽  
Romana Spitzer ◽  
Martin Tollinger ◽  
Robert Konrat ◽  
Christoph Kreutz
Keyword(s):  
Nmr Spectroscopy ◽  
Relaxation Dispersion ◽  
Rna Dynamics ◽  
Cpmg Relaxation Dispersion ◽  
Relaxation Dispersion Nmr

scholarly journals Binding Kinetics of Histone Chaperone Chz1 and Variant Histone H2A.Z-H2B by Relaxation Dispersion NMR Spectroscopy

2009 ◽  
Vol 387 (1) ◽  
pp. 1-9 ◽  
Author(s):  
D. Flemming Hansen ◽  
Zheng Zhou ◽  
Haniqiao Feng ◽  
Lisa M. Miller Jenkins ◽  
Yawen Bai ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Histone Chaperone ◽  
Relaxation Dispersion ◽  
Binding Kinetics ◽  
Relaxation Dispersion Nmr ◽  
Kinetics Of
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