Fine structure of the adrenocortical homologue in the north American eel and modifications following seawater adaptation

1979 ◽  
Vol 193 (2) ◽  
pp. 213-231 ◽  
Author(s):  
Tapan Kumar Bhattacharyya ◽  
David Gordon Butler
1977 ◽  
Vol 55 (2) ◽  
pp. 438-447 ◽  
Author(s):  
W. C. Hulbert ◽  
M. P. Komourdjian ◽  
T. W. Moon ◽  
J. C. Fenwick

Studies of the ultrastructure of the trophozoite (plasmodium) and the process of sporogony and polar capsule development of Myxidium zealandicum from gill and kidney of the North American eel Anguilla rostrata are described. Although the basic steps and characteristics of sporogony are similar to those previously reported for other myxosporidians, we report three new and significant observations in this study: (1) the identification of a uninucleate germ cell for the binucleate sporocyst; (2) the asynchronous maturation of polar capsules within a single sporocyst; and (3) the existence of a mitochondrial layer at the periphery of the Plasmodium that may have a transport function. We also observed cylindrical and spherical structures, either of which might be the primordium of the polar capsule, but we were unable to resolve this problem. These observations are discussed with reference to previous descriptions of myxosporidians.


1977 ◽  
Vol 55 (1) ◽  
pp. 52-59 ◽  
Author(s):  
M. P. Komourdjian ◽  
W. C. Hulbert ◽  
J. C. Fenwick ◽  
T. W. Moon

Myxidium zealandicum Hine, 1975 is described from gills and kidney of the North American eel Anguilla rostrata collected from the St. Lawrence River near Quebec City and Cornwall. Cysts of M. zealandicum on gills measured up to 1 by 2 mm and in kidneys up to 15 by 20 mm. In addition to single spherical cysts, several polymorphous forms were also observed on the gills. Polymorphous cysts were not found in the kidney. Different stages of spore development were evident in gill cysts and were differentiated by means of a lead hematoxylin – fast green stain. Number and pattern of spore striations were examined by scanning electron microscopy and were highly variable. The invasion of the parasite into kidney tissue appeared to result in less physiological damage to the host than did gill invasion. The existence of this parasite, previously found in eel species in New Zealand, in a North American eel species is discussed.


Author(s):  
Tapan Kumar Bhattacharyya ◽  
David Gordon Butler

The teleost fishes have been extensively utilized to explore the biochemical nature of adrenocortical steroids, pathways of steroidogenesis and the metabolic role of adrenocortical steroids. The adrenocortical homologue (AH) has been localized in more than two hundred species of teleosts, although the fine structure of teleost AH has been studied only in the goldfish. It seemed to be of interest to investigate the normal fine structure of this endocrine organ in another zoologically important teleost species, the north American eel (Anguilla rostrata) as a baseline for experimental studies. This animal being a euryhaline species, the AH ultrastructure of animals exposed to artificial seawater for a long period (1 1/2 years) was also examined.Mature female silver eels held in freshwater(FW) and seawater(SW) aquaria were anesthetized and perfused with glutaraldehyde in phosphate buffer or formaldehyde-glutaraldehyde in cacodylate buffer, and glutaraldehyde containing 0. 2% digitonin for demonstration of cholesterol.


2006 ◽  
Vol 175 (4S) ◽  
pp. 511-512
Author(s):  
David G. McLeod ◽  
Ira Klimberg ◽  
Donald Gleason ◽  
Gerald Chodak ◽  
Thomas Morris ◽  
...  

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