Ultrastructural study of the adrenocortical homologue in freshwater and seawater adapted eels
The teleost fishes have been extensively utilized to explore the biochemical nature of adrenocortical steroids, pathways of steroidogenesis and the metabolic role of adrenocortical steroids. The adrenocortical homologue (AH) has been localized in more than two hundred species of teleosts, although the fine structure of teleost AH has been studied only in the goldfish. It seemed to be of interest to investigate the normal fine structure of this endocrine organ in another zoologically important teleost species, the north American eel (Anguilla rostrata) as a baseline for experimental studies. This animal being a euryhaline species, the AH ultrastructure of animals exposed to artificial seawater for a long period (1 1/2 years) was also examined.Mature female silver eels held in freshwater(FW) and seawater(SW) aquaria were anesthetized and perfused with glutaraldehyde in phosphate buffer or formaldehyde-glutaraldehyde in cacodylate buffer, and glutaraldehyde containing 0. 2% digitonin for demonstration of cholesterol.