Histochemical and elemental localization of calcium in the granular cell subapical granules of the amphibian urinary bladder epithelium

1987 ◽  
Vol 218 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Walter L. Davis ◽  
Ruth Gwendolyn Jones ◽  
H. K. Hagler ◽  
Gene R. Farmer ◽  
David B. P. Goodman
Keyword(s):  
Urinary Bladder ◽  
Granular Cell ◽  
Bladder Epithelium ◽  
Amphibian Urinary Bladder

Effect of verapamil on cytoplasmic distribution of granules and microfilaments in amphibian urinary bladder

1988 ◽  
Vol 46 ◽  
pp. 324-325
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Morphological Changes ◽  
Granular Cell ◽  
Cytosolic Calcium ◽  
Calcium Storage ◽  
Amphibian Urinary Bladder ◽  
Vesicular Membrane ◽  
High Concentrations ◽  
Cytoskeletal System

The amphibian urinary bladder has been used as a ‘model’ system for studies of the mechanism of action of antidiuretic hormone (ADH) in stimulating transepithelial water flow. The increase in water permeability is accompanied by morphological changes that include the stimulation of apical microvilli, mobilization of microtubules and microfilaments and vesicular membrane fusion events . It has been shown that alterations in the cytosolic calcium concentrations can inhibit ADH transmembrane water flow and induce alterations in the epithelial cell cytomorphology, including the cytoskeletal system . Recently, the subapical granules of the granular cell in the amphibian urinary bladder have been shown to contain high concentrations of calcium, and it was suggested that these cytoplasmic constituents may act as calcium storage sites for intracellular calcium homeostasis. The present study utilizes the calcium antagonist, verapamil, to examine the effect of calcium deprivation on the cytomorphological features of epithelial cells from amphibian urinary bladder, with particular emphasis on subapical granule and microfilament distribution.

Activation of actin-containing microfilaments by vasopressin in the amphibian urinary bladder epithelium: A fluorescent study using NBD-phallacidin

1985 ◽  
Vol 211 (3) ◽  
pp. 239-245 ◽  
Author(s):  
Walter L. Davis ◽  
Ruth Gwendolyn Jones ◽  
Phillip C. Richemont ◽  
David B. P. Goodman
Keyword(s):  
Urinary Bladder ◽  
Bladder Epithelium ◽  
Amphibian Urinary Bladder

76 and 14 kDa polypeptides, two major components released from amphibian urinary bladder epithelium. Localization and potential role

1989 ◽  
Vol 66 (1-2) ◽  
pp. 131-143 ◽  
Author(s):  
A. Dassouli ◽  
R. Gobin ◽  
J. Grossetete ◽  
M. Rouchon ◽  
P. Ripoche ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Potential Role ◽  
Bladder Epithelium ◽  
Amphibian Urinary Bladder

Immunolocalization of secretory protein-I or chromogranin A in amphibian urinary bladder granular cell granules

1990 ◽  
Vol 14 (7) ◽  
pp. 601-612 ◽  
Author(s):  
W DAVIS ◽  
K SCHMID ◽  
J HUETTNER ◽  
G FARMER ◽  
B JACOBY ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Chromogranin A ◽  
Granular Cell ◽  
Secretory Protein ◽  
Amphibian Urinary Bladder

Isolated epithelial cells from amphibian urinary bladder express functional gap junctional hemichannels

1999 ◽  
Vol 276 (1) ◽  
pp. C279-C284 ◽  
Author(s):  
Carlos G. Vanoye ◽  
Leoncio A. Vergara ◽  
Luis Reuss
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Cell Damage ◽  
Divalent Cations ◽  
Membrane Conductance ◽  
Bladder Epithelium ◽  
Necturus Maculosus ◽  
Pathological Conditions ◽  
Amphibian Urinary Bladder ◽  
Gap Junctional

Exposure of the urinary bladder epithelium of Necturus maculosus (NUB) to protease and collagenase yields ∼50% isolated polarized cells. These cells express a membrane current slowly activated by depolarization or by removal of external divalent cations. The biophysical and pharmacological properties of the current are largely consistent with those of gap junctional hemichannels. After removal of divalent cations, the cells can also be loaded with 5(6)-carboxyfluorescein, a hydrophilic fluorescent anionic dye, and exposure to dye reduces the current in a manner dependent on membrane voltage and side of application. In contrast, Necturus gallbladder (NGB) cells exhibit no membrane conductance attributable to gap junctional hemichannels, although previous studies reveal the persistence of gap junction plaques on the plasma membrane. We conclude that functional gap junctional hemichannels can be expressed on the surface of certain isolated epithelial cells and that this is not a necessary consequence of the isolation procedure. These structures may contribute to cell damage under pathological conditions involving cell detachment.

Apical material extracted from amphibian urinary bladder epithelium by enzymes and detergent treatment

1985 ◽  
Vol 55 (3) ◽  
pp. 191-197 ◽  
Author(s):  
S. Bidet ◽  
V. Berthonaud ◽  
R. Gobin ◽  
J. Chevalier ◽  
J. Bourguet ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Bladder Epithelium ◽  
Amphibian Urinary Bladder ◽  
Detergent Treatment

scholarly journals Electron microscopic cytochemical localization of adenylate cyclase in amphibian urinary bladder epithelium: effects of antidiuretic hormone.

1987 ◽  
Vol 35 (1) ◽  
pp. 103-111 ◽  
Author(s):  
W L Davis ◽  
R G Jones ◽  
D B Goodman
Keyword(s):  
Urinary Bladder ◽  
Adenylate Cyclase ◽  
Antidiuretic Hormone ◽  
Toad Urinary Bladder ◽  
Osmotic Gradient ◽  
Bladder Epithelium ◽  
Electron Microscopic ◽  
Cytochemical Localization ◽  
Amphibian Urinary Bladder ◽  
Hormone Sensitive

A cytochemical technique for electron microscopic localization of adenylate cyclase was used to identify this enzyme in quiescent and hormone-stimulated toad urinary bladder epithelium. In the absence of vasopressin (antidiuretic hormone), adenylate cyclase was detected along the outer surface of the basolateral plasma membranes of granular cells, mitochondria-rich cells, and basal cells, the major cell types comprising the hormone-sensitive urinary epithelium. In the presence of antidiuretic hormone, the basolateral precipitates were markedly increased. The latter was true for both tissues incubated in the presence of an osmotic gradient and those stimulated in the absence of such a gradient. A significant mucosal reaction was never seen. Such data indicate that the hormone receptors for vasopressin are located along the basolateral membranes of all epithelial cells comprising the mucosal hormone-sensitive epithelium. All cells of the epithelium also demonstrate a vasopressin-sensitive adenylate cyclase. We discuss possible mechanisms that attempt to integrate the cytochemical data into an overall scheme for the physiological action of this hormone on amphibian urinary bladder.

Initial effects of cyclophosphamide on urinary bladder epithelium in the rat

Pathology ◽  
1974 ◽  
Vol 6 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Mary E. Schultz ◽  
Michael W. Weldon
Keyword(s):  
Urinary Bladder ◽  
Bladder Epithelium
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