Eugenol carbonate activity against Plasmodium falciparum , Leishmania braziliensis , and Trypanosoma cruzi

2021 ◽  
Author(s):  
Camila M. Clemente ◽  
Tatiana Pineda ◽  
Lina M. Yepes ◽  
Yulieth Upegui ◽  
Daniel A. Allemandi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Trypanosoma Cruzi ◽  
Leishmania Braziliensis

Lipid biosynthesis pathways as chemotherapeutic targets in kinetoplastid parasites

Parasitology ◽  
1997 ◽  
Vol 114 (7) ◽  
pp. 91-99 ◽  
Author(s):  
J. A. URBINA
Keyword(s):  
Trypanosoma Cruzi ◽  
Recent Work ◽  
Chagas Disease ◽  
Pneumocystis Carinii ◽  
Lipid Biosynthesis ◽  
Sterol Biosynthesis ◽  
Leishmania Braziliensis ◽  
Biosynthesis Inhibitors

Inhibitors of sterol and phospholipid biosynthesis in kinetoplastid parasites such as Trypanosoma cruzi, the causative agent of Chagas' disease, and different species of Leishmania have potent and selective activity as chemotherapeutic agents in vitro and in vivo. Recent work with the sterol C14α-demethylase inhibitor D0870, a bis triazole derivative, showed that this compound is capable of inducing radical parasitological cure in murine models of both acute and chronic Chagas' disease. Other inhibitors of this type, such as SCH 56592, have also shown curative, rather than suppressive, activity against T. cruzi in these models. Leishmania species have different susceptibilities to sterol biosynthesis inhibitors, both in vitro and in vivo. Leishmania braziliensis promastigotes, naturally resistant to C14α-demethylase inhibitors such as ketoconazole and D0870, were susceptible to these drugs when used in combination with the squalene epoxidase inhibitor terbinafine. Inhibitors of Δ24(25) sterol methyl transferase have been shown to act as potent antiproliferative agents against Trypanosoma cruzi, both in vitro and in vivo. New inhibitors of this type which show enhanced activity and novel mechanisms of action have been synthesized. Recent work has also demonstrated that this type of enzyme inhibitors can block sterol biosynthesis and cell proliferation in Pneumocystis carinii, a fungal pathogen which had previously been found resistant to other sterol biosynthesis inhibitors. Ajoene, an antiplatelet compound derived from garlic, was shown to have potent antiproliferative activity against epimastigotes and amastigotes of Trypanosoma cruzi in vitro; this activity was associated with a significant alteration of the phospholipid composition of the cells with no significant effects on the sterol content. In addition, alkyllsophospholipids such as ilmofosine, miltefosine and edelfosine have been shown to block the proliferation of T. cruzi and Leishmania and alter both the phospholipid and sterol composition. These results indicate the potential of lipid biosynthesis inhibitors as useful therapeutic agents in the treatment of leishmaniasis and Chagas' disease.

scholarly journals Antiparasitic Metabolites from Hyptis brevipes, a Tacana Medicinal Plant

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400 ◽  
Author(s):  
Ivan Limachi ◽  
Claudia Condo ◽  
Camila Palma ◽  
Nelida Nina ◽  
Efrain Salamanca ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Nmr Spectroscopy ◽  
Medicinal Plants ◽  
Medicinal Plant ◽  
Trypanosoma Cruzi ◽  
Hela Cells ◽  
Giardia Lamblia ◽  
Structure Elucidation ◽  
Intestinal Parasites ◽  
Active Constituents

A bioassay screening against protozoa parasites of several Tacana medicinal plants gave Hyptis brevipes (Id'ene eidhue), traditionally used as decoction for intestinal parasites, as the most active extract. In this work we did a bioguided isolation of active constituents found in leaves. Structure elucidation was carried out by NMR spectroscopy and MS spectrometry analyses. Active constituents showed differentiated activity towards Giardia lamblia, Trypanosoma cruzi, several Leishmania strains, Plasmodium falciparum and cytotoxicity against HeLa cells. Brevipolide H (1) was the less cytotoxic and best antiparasitic, while the catechol derivative (2) the most active and cytotoxic.

scholarly journals Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis

1996 ◽  
Vol 38 (3) ◽  
pp. 177-185 ◽  
Author(s):  
Ana de Cássia Vexenat ◽  
Jaime M. Santana ◽  
Antonio R.L. Teixeira
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Cross Reactivity ◽  
Antigenic Determinants ◽  
Leishmania Braziliensis ◽  
Leishmania Chagasi ◽  
Mucocutaneous Leishmaniasis ◽  
Kala Azar ◽  
Homologous Antigen ◽  
Positive Results

We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100% positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100% positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95% of Kala-azar and 35% of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15% of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.

scholarly journals Trypanocidal and Leishmanicidal Properties of Substitution-Containing Chalcones

2003 ◽  
Vol 47 (4) ◽  
pp. 1449-1451 ◽  
Author(s):  
Fabiane Lunardi ◽  
Michel Guzela ◽  
Andrea T. Rodrigues ◽  
Rogério Corrêa ◽  
Iriane Eger-Mangrich ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Toxic Effect ◽  
Leishmania Braziliensis ◽  
In Vitro Growth ◽  
Dependent Inhibition

ABSTRACT Ten chalcones were synthesized and tested as potential leishmanicidal and trypanocidal agents. All tested compounds caused concentration-dependent inhibition of the in vitro growth of Leishmania braziliensis and Trypanosoma cruzi with no significant toxic effect towards host macrophages. Our results show that the positions of the substituents seem to be critical for their antiprotozoal activities.

scholarly journals Intracellular Ca2+ Signaling in Protozoan Parasites: An Overview with a Focus on Mitochondria

2021 ◽  
Vol 22 (1) ◽  
pp. 469
Author(s):  
Pedro H. Scarpelli ◽  
Mateus F. Pecenin ◽  
Celia R. S. Garcia
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Plasmodium Falciparum ◽  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Cell Cycle Control ◽  
Endoplasmatic Reticulum ◽  
Protozoan Parasites ◽  
Cellular Functions ◽  
Higher Eukaryotes

Ca2+ signaling has been involved in controling critical cellular functions such as activation of proteases, cell death, and cell cycle control. The endoplasmatic reticulum plays a significant role in Ca2+ storage inside the cell, but mitochondria have long been recognized as a fundamental Ca2+ pool. Protozoan parasites such as Plasmodium falciparum, Toxoplasma gondii, and Trypanosoma cruzi display a Ca2+ signaling toolkit with similarities to higher eukaryotes, including the participation of mitochondria in Ca2+-dependent signaling events. This review summarizes the most recent knowledge in mitochondrial Ca2+ signaling in protozoan parasites, focusing on the mechanism involved in mitochondrial Ca2+ uptake by pathogenic protists.

scholarly journals Anticuerpos de Trypanosoma cruzi, Leishmania mexicana y Leishmania braziliensis en perros domiciliados de Tabasco, México

2017 ◽  
pp. 5829-5836
Author(s):  
Guadalupe Arjona J ◽  
Maritza Zaragoza V ◽  
Claudia Zaragoza V ◽  
Ricardo García Herrera ◽  
Manuel Sánchez M ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Leishmania Braziliensis ◽  
Leishmania Mexicana

Objetivo. Determinar la frecuencia de anticuerpos circulantes de Trypanosoma cruzi (T. cruzi), Leishmania mexicana (L. mexicana) y Leishmania braziliensis (L. braziliensis) en una población de perros usando ELISA Fe-SOD y Western blot en la región Chontalpa del estado de Tabasco, México. Materiales y métodos. Para este estudio se obtuvieron 119 sueros de perros domiciliados, con el consentimiento previo de los propietarios. Los sueros fueron analizados para detectar anticuerpos contra T. cruzi, L. mexicana, y L. braziliensis, usando como prueba diagnóstica ELISA-sod y Western Blot. La fracción antigénica utilizada en las dos pruebas fue la Fe-SOD excretada por las especies de Trypanosoma y Leishmania. Resultados. La frecuencia obtenida en este estudio fue de 3.36% para T. cruzi, 9.24% para L. mexicana y 10.08% L. braziliensis. Conclusiones. El presente estudio demostró la presencia de anticuerpos para estos parásitos en la región Chontalpa del estado de Tabasco, México.

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