scholarly journals The fate of Leishmania braziliensis, L. donovani and Trypanosoma cruzi in diffusion chambers implanted into hamsters and mice. A preliminary study.

1987 ◽  
Vol 15 (2) ◽  
pp. 97-104
Author(s):  
YOSHIHISA HASHIGUCHI ◽  
MASATO FURUYA ◽  
YOSHISUKE OKAMURA
Keyword(s):  
Trypanosoma Cruzi ◽  
Leishmania Braziliensis ◽  
Diffusion Chambers ◽  
Preliminary Study

Eugenol carbonate activity against Plasmodium falciparum , Leishmania braziliensis , and Trypanosoma cruzi

2021 ◽  
Author(s):  
Camila M. Clemente ◽  
Tatiana Pineda ◽  
Lina M. Yepes ◽  
Yulieth Upegui ◽  
Daniel A. Allemandi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Trypanosoma Cruzi ◽  
Leishmania Braziliensis

Lipid biosynthesis pathways as chemotherapeutic targets in kinetoplastid parasites

Parasitology ◽  
1997 ◽  
Vol 114 (7) ◽  
pp. 91-99 ◽  
Author(s):  
J. A. URBINA
Keyword(s):  
Trypanosoma Cruzi ◽  
Recent Work ◽  
Chagas Disease ◽  
Pneumocystis Carinii ◽  
Lipid Biosynthesis ◽  
Sterol Biosynthesis ◽  
Leishmania Braziliensis ◽  
Biosynthesis Inhibitors

Inhibitors of sterol and phospholipid biosynthesis in kinetoplastid parasites such as Trypanosoma cruzi, the causative agent of Chagas' disease, and different species of Leishmania have potent and selective activity as chemotherapeutic agents in vitro and in vivo. Recent work with the sterol C14α-demethylase inhibitor D0870, a bis triazole derivative, showed that this compound is capable of inducing radical parasitological cure in murine models of both acute and chronic Chagas' disease. Other inhibitors of this type, such as SCH 56592, have also shown curative, rather than suppressive, activity against T. cruzi in these models. Leishmania species have different susceptibilities to sterol biosynthesis inhibitors, both in vitro and in vivo. Leishmania braziliensis promastigotes, naturally resistant to C14α-demethylase inhibitors such as ketoconazole and D0870, were susceptible to these drugs when used in combination with the squalene epoxidase inhibitor terbinafine. Inhibitors of Δ24(25) sterol methyl transferase have been shown to act as potent antiproliferative agents against Trypanosoma cruzi, both in vitro and in vivo. New inhibitors of this type which show enhanced activity and novel mechanisms of action have been synthesized. Recent work has also demonstrated that this type of enzyme inhibitors can block sterol biosynthesis and cell proliferation in Pneumocystis carinii, a fungal pathogen which had previously been found resistant to other sterol biosynthesis inhibitors. Ajoene, an antiplatelet compound derived from garlic, was shown to have potent antiproliferative activity against epimastigotes and amastigotes of Trypanosoma cruzi in vitro; this activity was associated with a significant alteration of the phospholipid composition of the cells with no significant effects on the sterol content. In addition, alkyllsophospholipids such as ilmofosine, miltefosine and edelfosine have been shown to block the proliferation of T. cruzi and Leishmania and alter both the phospholipid and sterol composition. These results indicate the potential of lipid biosynthesis inhibitors as useful therapeutic agents in the treatment of leishmaniasis and Chagas' disease.

scholarly journals Cross-reactivity of antibodies in human infections by the kinetoplastid protozoa Trypanosoma cruzi, Leishmania chagasi and Leishmania (Viannia) braziliensis

1996 ◽  
Vol 38 (3) ◽  
pp. 177-185 ◽  
Author(s):  
Ana de Cássia Vexenat ◽  
Jaime M. Santana ◽  
Antonio R.L. Teixeira
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Cross Reactivity ◽  
Antigenic Determinants ◽  
Leishmania Braziliensis ◽  
Leishmania Chagasi ◽  
Mucocutaneous Leishmaniasis ◽  
Kala Azar ◽  
Homologous Antigen ◽  
Positive Results

We have detected antibodies, in the sera of Chagas disease, Kala-azar and Mucocutaneous leishmaniasis patients, that bind multiple antigens shared between the three causative agents. The Chagas disease sera showed 98 to 100% positive results by ELISA when the Leishmania braziliensis and Leishmania chagasi antigens were used, respectively. The Kala-azar sera showed 100% positive results with Trypanosoma cruzi or L. braziliensis antigens by immunofluorescence assays. The antibodies in the sera of Mucocutaneous leishmaniasis patients showed 100% positive results by ELISA assays with T. cruzi or L. chagasi antigens. Furthermore, the direct agglutination of L. chagasi promastigotes showed that 95% of Kala-azar and 35% of Mucocutaneous leishmaniasis sera agglutinated the parasite in dilutions above 1:512. In contrast, 15% of Chagas sera agglutinated the parasite in dilutions 1:16 and below. Western blot analysis showed that the Chagas sera that formed at least 24 bands with the T. cruzi also formed 13 bands with the L. chagasi and 17 bands with the L. braziliensis. The Kala-azar sera that recognized at least 29 bands with the homologous antigen also formed 14 bands with the T. cruzi and 10 bands with the L. braziliensis antigens. Finally, the Mucocutaneous leishmaniasis sera that formed at least 17 bands with the homologous antigen also formed 10 bands with the T. cruzi and four bands with the L. chagasi antigens. These results indicate the presence of common antigenic determinants in several protozoal proteins and, therefore, explain the serologic cross-reactions reported here.

scholarly journals Trypanocidal and Leishmanicidal Properties of Substitution-Containing Chalcones

2003 ◽  
Vol 47 (4) ◽  
pp. 1449-1451 ◽  
Author(s):  
Fabiane Lunardi ◽  
Michel Guzela ◽  
Andrea T. Rodrigues ◽  
Rogério Corrêa ◽  
Iriane Eger-Mangrich ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Toxic Effect ◽  
Leishmania Braziliensis ◽  
In Vitro Growth ◽  
Dependent Inhibition

ABSTRACT Ten chalcones were synthesized and tested as potential leishmanicidal and trypanocidal agents. All tested compounds caused concentration-dependent inhibition of the in vitro growth of Leishmania braziliensis and Trypanosoma cruzi with no significant toxic effect towards host macrophages. Our results show that the positions of the substituents seem to be critical for their antiprotozoal activities.

scholarly journals Anticuerpos de Trypanosoma cruzi, Leishmania mexicana y Leishmania braziliensis en perros domiciliados de Tabasco, México

2017 ◽  
pp. 5829-5836
Author(s):  
Guadalupe Arjona J ◽  
Maritza Zaragoza V ◽  
Claudia Zaragoza V ◽  
Ricardo García Herrera ◽  
Manuel Sánchez M ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Western Blot ◽  
Leishmania Braziliensis ◽  
Leishmania Mexicana

Objetivo. Determinar la frecuencia de anticuerpos circulantes de Trypanosoma cruzi (T. cruzi), Leishmania mexicana (L. mexicana) y Leishmania braziliensis (L. braziliensis) en una población de perros usando ELISA Fe-SOD y Western blot en la región Chontalpa del estado de Tabasco, México. Materiales y métodos. Para este estudio se obtuvieron 119 sueros de perros domiciliados, con el consentimiento previo de los propietarios. Los sueros fueron analizados para detectar anticuerpos contra T. cruzi, L. mexicana, y L. braziliensis, usando como prueba diagnóstica ELISA-sod y Western Blot. La fracción antigénica utilizada en las dos pruebas fue la Fe-SOD excretada por las especies de Trypanosoma y Leishmania. Resultados. La frecuencia obtenida en este estudio fue de 3.36% para T. cruzi, 9.24% para L. mexicana y 10.08% L. braziliensis. Conclusiones. El presente estudio demostró la presencia de anticuerpos para estos parásitos en la región Chontalpa del estado de Tabasco, México.

scholarly journals Activity of Estafietin and Analogues on Trypanosoma cruzi and Leishmania braziliensis

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1209 ◽  
Author(s):  
Valeria Sülsen ◽  
Emilio Lizarraga ◽  
Orlando Elso ◽  
Natacha Cerny ◽  
Andrés Sanchez Alberti ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Biological Activities ◽  
Sesquiterpene Lactones ◽  
Leishmania Braziliensis ◽  
Antiprotozoal Activity ◽  
Plant Metabolites ◽  
Antiparasitic Activity ◽  
Naturally Occurring ◽  
Wide Range ◽  
Ic50 Values

Sesquiterpene lactones are naturally occurring compounds mainly found in the Asteraceae family. These types of plant metabolites display a wide range of biological activities, including antiprotozoal activity and are considered interesting structures for drug discovery. Four derivatives were synthesized from estafietin (1), isolated from Stevia alpina (Asteraceae): 11βH,13-dihydroestafietin (2), epoxyestafietin (3a and 3b), 11βH,13-methoxyestafietin, (4) and 11βH,13-cianoestafietin. The antiprotozoal activity against Trypanosoma cruzi and Leishmania braziliensis of these compounds was evaluated. Epoxyestafietin was the most active compound against T. cruzi trypomastigotes and amastigotes (IC50 values of 18.7 and 2.0 µg/mL, respectively). Estafietin (1) and 11βH,13-dihydroestafietin (2) were the most active and selective compounds on L. braziliensis promastigotes (IC50 values of 1.0 and 1.3 μg/mL, respectively). The antiparasitic activity demonstrated by estafietin and some of its derivatives make them promising candidates for the development of effective compounds for the treatment of Chagas disease and leihsmaniasis.

scholarly journals SARs for the Antiparasitic Plant Metabolite Pulchrol. Part 2: B- and C-Ring Substituents

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4510
Author(s):  
Paola Terrazas ◽  
Sophie Manner ◽  
Olov Sterner ◽  
Marcelo Dávila ◽  
Alberto Giménez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Neglected Tropical Diseases ◽  
Leishmania Species ◽  
Positive Control ◽  
Leishmania Braziliensis ◽  
General Tendency ◽  
Antiparasitic Activity ◽  
Plant Metabolite ◽  
Derivatives Of

Neglected tropical diseases affect most of the underprivileged populations in tropical countries. Among these are chagas and leishmaniasis, present mainly in South and Central America, Africa and East Asia. Current treatments are long and have severe adverse effects, therefore there is a strong need to develop alternatives. In this study, we base our research on the plant metabolite pulchrol, a natural benzochromene which has been shown to possess antiparasitic activity against Trypanosoma and Leishmania species. In a recent study, we investigated how changes in the benzyl alcohol functionality affected the antiparasitic activity, but the importance of B- and C-ring substituents is not understood. Fifteen derivatives of pulchrol with different substituents in positions 1, 2, 3, and 6 while leaving the A-ring intact, were therefore prepared by total synthesis, assayed, and compared with pulchrol and positive controls. The generated series and parental molecule were tested in vitro for antiparasitic activity against Trypanosoma cruzi, Leishmania braziliensis, and L. amazonensis, and cytotoxicity using RAW cells. Substantial differences in the activity of the compounds synthesized were observed, of which some were more potent towards Trypanosoma cruzi than the positive control benznidazole. A general tendency is that alkyl substituents improve the potency, especially when positioned on C-2.

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