Biochemical characterization of amyloid derived from the variable region of thek light chain subgroup III

1990 ◽  
Vol 33 (6) ◽  
pp. 880-884 ◽  
Author(s):  
Maria M. Picken ◽  
Gloria R. Gallo ◽  
Waldemar Pruzanski ◽  
Blas Frangione
1987 ◽  
Vol 166 (6) ◽  
pp. 1900-1905 ◽  
Author(s):  
P P Chen ◽  
D L Robbins ◽  
F R Jirik ◽  
T J Kipps ◽  
D A Carson

Previously, we isolated a Vk gene (Humkv325) from a human placenta that encodes RF light chains bearing the PSL2 and PSL3 CRI markers. Here we report the isolation and characterization of a second human Vk gene (Humkv328) that can be used for RF synthesis. This Vk gene probably encodes at least two 6B6.6 CRI+ RF light chains (Les and Pom) from unrelated subjects, and thus may be related to the light chain-associated 6B6.6 CRI.


Leukemia ◽  
1998 ◽  
Vol 12 (4) ◽  
pp. 601-609 ◽  
Author(s):  
H Kiyoi ◽  
K Naito ◽  
R Ohno ◽  
H Saito ◽  
T Naoe

2006 ◽  
Vol 71 (2) ◽  
pp. 113-119 ◽  
Author(s):  
M. E. Gasparian ◽  
V. G. Ostapchenko ◽  
D. A. Dolgikh ◽  
M. P. Kirpichnikov

2020 ◽  
Vol 7 ◽  
Author(s):  
Monica L. Fernández-Quintero ◽  
Katharina B. Kroell ◽  
Martin C. Heiss ◽  
Johannes R. Loeffler ◽  
Patrick K. Quoika ◽  
...  

Fab consist of a heavy and light chain and can be subdivided into a variable (VH and VL) and a constant region (CH1 and CL). The variable region contains the complementarity-determining region (CDR), which is formed by six hypervariable loops, shaping the antigen binding site, the paratope. Apart from the CDR loops, both the elbow angle and the relative interdomain orientations of the VH–VL and the CH1–CL domains influence the shape of the paratope. Thus, characterization of the interface and elbow angle dynamics is essential to antigen specificity. We studied nine antigen-binding fragments (Fab) to investigate the influence of affinity maturation, antibody humanization, and different light-chain types on the interface and elbow angle dynamics. While the CDR loops reveal conformational transitions in the micro-to-millisecond timescale, both the interface and elbow angle dynamics occur on the low nanosecond timescale. Upon affinity maturation, we observe a substantial rigidification of the VH and VL interdomain and elbow-angle flexibility, reflected in a narrower and more distinct distribution. Antibody humanization describes the process of grafting non-human CDR loops onto a representative human framework. As the antibody framework changes upon humanization, we investigated if both the interface and the elbow angle distributions are changed or shifted. The results clearly showed a substantial shift in the relative VH–VL distributions upon antibody humanization, indicating that different frameworks favor distinct interface orientations. Additionally, the interface and elbow angle dynamics of five antibody fragments with different light-chain types are included, because of their strong differences in elbow angles. For these five examples, we clearly see a high variability and flexibility in both interface and elbow angle dynamics, highlighting the fact that Fab interface orientations and elbow angles interconvert between each other in the low nanosecond timescale. Understanding how the relative interdomain orientations and the elbow angle influence antigen specificity, affinity, and stability has broad implications in the field of antibody modeling and engineering.


2012 ◽  
Vol 91 (8) ◽  
pp. 1251-1255 ◽  
Author(s):  
Jian Li ◽  
Zhen Huang ◽  
Ming-hui Duan ◽  
Wei Zhang ◽  
Miao Chen ◽  
...  

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