Process development exploiting competitive adsorption‐based displacement effects in monoclonal antibody aggregate removal ‐ a new high throughput screening procedure for membrane chromatography

2021 ◽  
Author(s):  
Dominik Stein ◽  
Volkmar Thom ◽  
Jürgen Hubbuch
Keyword(s):  
Monoclonal Antibody ◽  
High Throughput ◽  
High Throughput Screening ◽  
Competitive Adsorption ◽  
Process Development ◽  
Screening Procedure ◽  
Membrane Chromatography ◽  
Displacement Effects

scholarly journals High Throughput Screening of Formulations to Optimize the Thermal Stability of Therapeutic Monoclonal Antibody

2014 ◽  
Vol 106 (2) ◽  
pp. 470a
Author(s):  
Elaine Kan ◽  
Anita Niedziela-Majka ◽  
Perry Weissburg ◽  
Scott Sellers ◽  
Roman Sakowicz
Keyword(s):  
Thermal Stability ◽  
Monoclonal Antibody ◽  
High Throughput ◽  
High Throughput Screening ◽  
Therapeutic Monoclonal Antibody ◽  
Thermal Stability Of

High-throughput Screening Applied to Process Development

ChemInform ◽  
2004 ◽  
Vol 35 (12) ◽  
Author(s):  
Oliver Brummer ◽  
Bernd Jandeleit ◽  
Tetsuo Uno ◽  
W. Henry Weinberg
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Process Development

scholarly journals Process Development for Cross‐flow Diafiltration‐based VLP Disassembly: A Novel High‐throughput Screening Approach

2021 ◽  
Author(s):  
Nils Hillebrandt ◽  
Philipp Vormittag ◽  
Annabelle Dietrich ◽  
Christina H. Wegner ◽  
Jürgen Hubbuch
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Process Development ◽  
Cross Flow ◽  
Screening Approach

Mixed Mode Chromatography, Complex Development for Large Opportunities

2018 ◽  
Vol 20 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Charlotte Cabanne ◽  
Xavier Santarelli
Keyword(s):  
Salt Tolerance ◽  
High Throughput ◽  
High Throughput Screening ◽  
Mixed Mode ◽  
Design Space ◽  
Process Development ◽  
Optimal Parameters ◽  
Purification Step ◽  
Liquid Handling ◽  
Complex Development

Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins. High-Throughput Screening (HTS) for Process Development should be done with the help of Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid handling automated workstation. Modeling of the results allows the choice of optimal parameters that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification step.

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