Mixed mode chromatography resins with salt tolerance, large design space and orthogonal
selectivity requires a slightly more complex development than traditional resins. It is important to screen
several ligands and several binding and elution conditions. This allows taking full advantage of these
resins. High-Throughput Screening (HTS) for Process Development should be done with the help of
Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid
handling automated workstation. Modeling of the results allows the choice of optimal parameters
that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification
step.
Process Development for Cross‐flow Diafiltration‐based VLP Disassembly: A Novel High‐throughput Screening Approach