Induced effect of Ca 2+ and Al 3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Thermostability of crude endoglucanase from Aspergillus fumigatus grown under solid state fermentation (SSF) and submerged fermentation (SmF)

Process Biochemistry ◽

10.1016/j.procbio.2009.12.011 ◽

2010 ◽

Vol 45 (5) ◽

pp. 641-646 ◽

Cited By ~ 79

Author(s):

Abdul A.N. Saqib ◽

Madiha Hassan ◽

Nahida Farooq Khan ◽

Shahjahan Baig

Keyword(s):

Solid State ◽

Aspergillus Fumigatus ◽

Solid State Fermentation ◽

Submerged Fermentation

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Statistical optimization of substrate, carbon and nitrogen source by response surface methodology for pectinase production using Aspergillus fumigatus MTCC 870 in submerged fermentation

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb09.186 ◽

2009 ◽

Vol 8 (22) ◽

pp. 6355-6363 ◽

Cited By ~ 6

Author(s):

Palaniyappan M ◽

Vijayagopal V ◽

Viswanathan Renuka ◽

Viruthagiri T

Keyword(s):

Response Surface Methodology ◽

Aspergillus Fumigatus ◽

Nitrogen Source ◽

Response Surface ◽

Submerged Fermentation ◽

Statistical Optimization ◽

Carbon And Nitrogen ◽

Pectinase Production

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Improvement of chaetominine production by tryptophan feeding and medium optimization in submerged fermentation of Aspergillus fumigatus CY018

Bioresources and Bioprocessing ◽

10.1186/s40643-016-0117-5 ◽

2016 ◽

Vol 3 (1) ◽

Cited By ~ 2

Author(s):

Yu-peng Zhang ◽

Rui-hua Jiao ◽

Yan-hua Lu ◽

Ling-yun Yao

Keyword(s):

Aspergillus Fumigatus ◽

Submerged Fermentation ◽

Medium Optimization

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Induced effect of Ca2+ and Al3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

10.21203/rs.3.rs-791080/v1 ◽

2021 ◽

Author(s):

Changqing Liu ◽

Tianwen Chen ◽

Jijie Chen ◽

Yuxuan Zhou ◽

Lina Deng ◽

...

Keyword(s):

Aspergillus Fumigatus ◽

Submerged Fermentation ◽

Large Scale ◽

Leukemia Cell ◽

Human Leukemia ◽

Biosynthesis Pathway ◽

Promoting Effect ◽

Intermediate Metabolites ◽

Fermentation Strategy ◽

Co Addition

Abstract Chaetominine (CHA), an alkaloid with biological activity produced by Aspergillus fumigatus CY018, has strong anticancer activity against the human leukemia cell. However, its further research for pharmacokinetics is limited by CHA production in submerged fermentation, which is a problem urgently awaiting solutions by effective biological strategies. In this work, Ca2+ and Al3+ exhibited a strong promoting effect on CHA production after multiple metal ions screening. Then, the concentration of Ca2+ and Al3+ and the addition time was optimized. The suitable conditions were 0.5 mM Ca2+ and 0.5 mM Al3+ at 120 h, in which the CHA production reached 58.5 and 49.45 mg/L with optimized condition of Ca2+ and Al3+ respectively. The intermediate metabolites, such as DAHP, chorismate and tryptophan, were increased obviously with addition of Ca2+ and Al3+. The activities of key enzymes of DAHPs, AroAs and TrpCs in CHA biosynthesis pathway were improved by 3.58-, 3.60- and 3.34-folds respectively. Meanwhile, the transcription levels of laeA, dahp, cs, and trpC were upregulated by 3.22-, 12.65-, 5.58- and 6.99-fold respectively by addition of Ca2+ and Al3+. Additionally, the fermentation strategy was successfully scaled up to 5 L reactor, in which CHA production could attain 75.6 mg/L at 336 h. This work demonstrated that Ca2+ and Al3+ co-addition was an effective strategy for increasing CHA production, and the information obtained might be useful to the further improvement of CHA fermentation on large scale.

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