Co-addition Strategy for Enhancement of Chaetominine from Submerged Fermentation of Aspergillus fumigatus CY018

2018 ◽  
Vol 186 (2) ◽  
pp. 384-399 ◽  
Author(s):  
Chang-Qing Liu ◽  
Zheng-Hua Pan ◽  
Fa-Liang An ◽  
Yan-Hua Lu
Keyword(s):  
Aspergillus Fumigatus ◽  
Submerged Fermentation ◽  
Co Addition

scholarly journals Induced effect of Ca2+ and Al3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

2021 ◽  
Author(s):  
Changqing Liu ◽  
Tianwen Chen ◽  
Jijie Chen ◽  
Yuxuan Zhou ◽  
Lina Deng ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Submerged Fermentation ◽  
Large Scale ◽  
Leukemia Cell ◽  
Human Leukemia ◽  
Biosynthesis Pathway ◽  
Promoting Effect ◽  
Intermediate Metabolites ◽  
Fermentation Strategy ◽  
Co Addition

Abstract Chaetominine (CHA), an alkaloid with biological activity produced by Aspergillus fumigatus CY018, has strong anticancer activity against the human leukemia cell. However, its further research for pharmacokinetics is limited by CHA production in submerged fermentation, which is a problem urgently awaiting solutions by effective biological strategies. In this work, Ca2+ and Al3+ exhibited a strong promoting effect on CHA production after multiple metal ions screening. Then, the concentration of Ca2+ and Al3+ and the addition time was optimized. The suitable conditions were 0.5 mM Ca2+ and 0.5 mM Al3+ at 120 h, in which the CHA production reached 58.5 and 49.45 mg/L with optimized condition of Ca2+ and Al3+ respectively. The intermediate metabolites, such as DAHP, chorismate and tryptophan, were increased obviously with addition of Ca2+ and Al3+. The activities of key enzymes of DAHPs, AroAs and TrpCs in CHA biosynthesis pathway were improved by 3.58-, 3.60- and 3.34-folds respectively. Meanwhile, the transcription levels of laeA, dahp, cs, and trpC were upregulated by 3.22-, 12.65-, 5.58- and 6.99-fold respectively by addition of Ca2+ and Al3+. Additionally, the fermentation strategy was successfully scaled up to 5 L reactor, in which CHA production could attain 75.6 mg/L at 336 h. This work demonstrated that Ca2+ and Al3+ co-addition was an effective strategy for increasing CHA production, and the information obtained might be useful to the further improvement of CHA fermentation on large scale.

scholarly journals Induced effect of Ca 2+ and Al 3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

2022 ◽  
Author(s):  
Changqing Liu ◽  
Tianwen Chen ◽  
Jijie Chen ◽  
Yuxuan Zhou ◽  
Lina Deng ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Submerged Fermentation

Nicht-invasiver Nachweis von Aspergillus fumigatus anhand des Atemwegskondensats von Patienten mit neutropenem Fieber

Pneumologie ◽  
2014 ◽  
Vol 68 (02) ◽  
Author(s):  
A Gutmann ◽  
J Koepke ◽  
C Aepinus ◽  
C Wilhem ◽  
A Burchert ◽  
...  
Keyword(s):  
Aspergillus Fumigatus

Effect of Al2O3 and SiO2 Metal Oxide Nanoparticles Blended with POME on Combustion, Performance and Emissions Characteristics of a Diesel Engine

2019 ◽  
Vol 16 (3) ◽  
pp. 6859-6873 ◽  
Author(s):  
M. A. Adzmi ◽  
A. Abdullah ◽  
Z. Abdullah ◽  
A. G. Mrwan
Keyword(s):  
Diesel Engine ◽  
Metal Oxide Nanoparticles ◽  
Peak Torque ◽  
Combustion Characteristic ◽  
Maximum Pressure ◽  
Single Cylinder ◽  
Engine Testing ◽  
Co Addition ◽  
Carbon Dioxide Co2 ◽  
Test Fuel

Evaluation of combustion characteristic, engine performances and exhaust emissions of nanoparticles blended in palm oil methyl ester (POME) was conducted in this experiment using a single-cylinder diesel engine. Nanoparticles used was aluminium oxide (Al2O3) and silicon dioxide (SiO2) with a portion of 50 ppm and 100 ppm. SiO2 and Al2O3 were blended in POME and labelled as PS50, PS100 and PA50, PA100, respectively. The data results for PS and PA fuel were compared to POME test fuel. Single cylinder diesel engine YANMAR TF120M attached with DEWESoft data acquisition module (DAQ) model SIRIUSi-HS was used in this experiment. Various engine loads of zero, 7 N.m, 14 Nm, 21 N.m and 28 N.m at a constant engine speed of 1800 rpm were applied during engine testing. Results for each fuel were obtained by calculating the average three times repetition of engine testing. Findings show that the highest maximum pressure of nanoparticles fuel increase by 16.3% compared to POME test fuel. Other than that, the engine peak torque and engine power show a significant increase by 43% and 44%, respectively, recorded during the PS50 fuel test. Meanwhile, emissions of nanoparticles fuel show a large decrease by 10% of oxide of nitrogen (NOx), 6.3% reduction of carbon dioxide (CO2) and a slight decrease of 0.02% on carbon monoxide (CO). Addition of nanoparticles in biodiesel show positive improvements when used in diesel engines and further details were discussed.  

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