Antioxidant Activity
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2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Mohd Aaqib Sheikh ◽  
Charanjiv Singh Saini ◽  
Harish Kumar Sharma

PurposeThe study was aimed to explore the potential impact of microwave heating (450 W for 2, 4, 6 and 8 min) on antioxidant activity, anti-nutritional factors, volatile and phenolic compounds of the plum kernels.Design/methodology/approachPlum kernels are rich in proteins, lipids and bioactive compounds that are mostly underused and undervalued.FindingsThe results showed that microwave heating at 450 W for 6 min significantly (p < 0.05) increased the antioxidant activity, total phenolic and flavonoid content, while the longer treatment time (450 W for 8 min) adversely affected the phenolic compounds. Most importantly, the anti-nutritional factors like amygdalin, hydrocyanic acid, phytic acid and tannin content were reduced up to 87.1, 84.7, 20.9 and 46.2%, respectively at 450 W for 6 min treatment conditions, which was confirmed from the larger shifts observed in FT-IR spectra near 1,157 cm−1. Microwave heating at 450 W for 6 min also proved beneficial in improving the bioavailability of volatile and phenolic compounds including chlorogenic acid, gallic acid, syringic acid, (+)-catechin, caffeic acid, ß-carotene, trans-ferulic acid, rutin trihydrate, 3,4-dihydroxybenzoic acid, tannic acid and quercetin by liberating them from the plant matrix.Originality/valueThe results thus indicate that controlled microwave heating could be an effective approach for the reduction of anti-nutritional factors besides leading to an overall improvement in antioxidant potential and volatile and phenolic compounds. This novel technological approach can proliferate the use of plum kernels in different diversified food formulations.

2021 ◽  
pp. 100177
M. Ramón-Sierra Jesús ◽  
A. Villanueva Marco ◽  
Yam-Puc Alejandro ◽  
Rodríguez-Mendiola Martha ◽  
Arias-Castro Carlos ◽  

Nur Syazwani Hanafi ◽  
Rosnani Hasham ◽  
Nor Zalina Othman ◽  
Mohamad Roji Sarmidi

The use of freeze drying process to prolong the shelf life of food products is considered mainstream in food industries due to its capability to retain the nutritional value. Citric acid and osmotic solution were used as a pre-treatment in this study to produce high-quality dried products. Four treatment groups were observed, including untreated (control), 1% citric acid (CA), 1% CA with 20% sugar solution (CAOD 20%), and 1% CA with 60% sugar solution (CAOD 60%). Physical properties such as moisture content; pH; total soluble solid; colour value; and bioactive compounds, such as vitamin C, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities were evaluated in freeze-dried pineapples. The result showed that CAOD 60% demonstrated a positive effect, with the least moisture content and the lowest value of colour changes (ΔE) at 5.69 ± 0.04% and 33.76 ± 2.17, respectively, as compared with that of other samples. In terms of phytochemical, the control and CA groups recorded higher retention of vitamin C, TPC, and TFC compared with other osmotic dehydration samples; however, there was no significant effect (p<0.05) on TPC and TFC. Interestingly, CAOD 60% possessed strong antioxidant activity, with the lowest IC50 value of 85.85 ± 5.93. From these findings, it was found that CAOD 60% resulted in positive effect on both the physical qualities and preservation of antioxidant activity, making it a recommended condition for pre-treatment in fruit preservation, which is essential in ensuring both the food security aspect and maintenance of its nutritional value.

Irini F. Strati ◽  
Panagiotis Tataridis ◽  
Adnan Shehadeh ◽  
Arhontoula Chatzilazarou ◽  
Vasileios Bartzis ◽  

Rosario Pardo-Botello ◽  
Fátima Chamizo-Calero ◽  
Olga Monago-Maraña ◽  
Raquel Rodríguez-Corchado ◽  
Rosa de la Torre-Carreras ◽  

AbstractThe hydrophilic and lipophilic antioxidant activities due to the main bioactive components present in Spanish tomato paste samples were studied, using standardized and fluorescent methods. After extraction, phenolic antioxidants (Folin-Ciocalteu method) and total antioxidant activity (TEAC assay) were evaluated, examining differences between hydrophilic and lipophilic extracts corresponding to different samples. Total fluorescence spectra of extracts (excitation-emission matrices, EEMs) were recorded in the front-face mode at two different ranges: 210–300 nm/310–390 nm, and 295–350 nm/380–480 nm, for excitation and emission, respectively, in the hydrophilic extracts. In the lipophilic extracts, the first range was 230–283 nm/290–340 nm, while the second range was 315–383 nm/390–500 nm for excitation and emission, respectively. EEMs from a set of 22 samples were analyzed by the second-order multivariate technique Parallel Factor Analysis (PARAFAC). Tentative assignation of the different components to the various fluorophores of tomato was tried, based on literature. Correlation between the antioxidant activity and score values retrieved for different components in PARAFAC model was obtained. The possibility of using EEMs-PARAFAC to evaluate antioxidant activity of hydrophilic and lipophilic compounds in these samples was examined, obtaining good results in accordance with the Folin-Ciocalteu and TEAC assays.

2021 ◽  
Vol 12 ◽  
Deepak Kasote ◽  
Rhowell N. Tiozon ◽  
Kristel June D. Sartagoda ◽  
Hameeda Itagi ◽  
Priyabrata Roy ◽  

Cereal grains and products provide calories globally. The health benefits of cereals attributed to their diverse phenolic constituents have not been systematically explored. Post-harvest processing, such as drying, storing, and milling cereals, can alter the phenolic concentration and influence the antioxidant activity. Furthermore, cooking has been shown to degrade thermo-labile compounds. This review covers several methods for retaining and enhancing the phenolic content of cereals to develop functional foods. These include using bioprocesses such as germination, enzymatic, and fermentation treatments designed to enhance the phenolics in cereals. In addition, physical processes like extrusion, nixtamalization, and parboiling are discussed to improve the bioavailability of phenolics. Recent technologies utilizing ultrasound, micro- or nano-capsule polymers, and infrared utilizing processes are also evaluated for their effectiveness in improving the phenolics content and bio-accessibility. We also present contemporary products made from pigmented cereals that contain phenolics.

2021 ◽  
Min Zhu ◽  
Yiwen Han ◽  
Xuechao Hu ◽  
Lujing Ren

Abstract Ergothioneine is a natural and safe antioxidant playing an important role in anti-aging and the prevention of various diseases. Mushrooms are the main dietary source of ergothioneine. This study aims to report a kind of medicinal mushroom Panus conchatus with great potential for the bioproduction of ergothioneine. Molasses and soy peptone could promote cell growth of Panus conchatus and enhance the accumulation of ergothioneine. Meanwhile, three precursors of cysteine, histidine and methionine were added to the broth, and the highest ergothioneine concentration of 148.79mg/L was obtained when adding 0.4 g/L cysteine. Finally, the crude ergothioneine extract was purified and further evaluated. The ergothioneine from Panus conchatus showed higher antioxidant activity than vitamin C and glutathione with good stability at lower pH environment. This research would provide a new method for the bioproduction of ergothioneine and lay foundation for the in-depth study about the exploration of natural products from Panus conchatus.

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