Highly sensitive HPLC method for the determination of galantamine in human plasma and urine through derivatization with dansyl chloride using fluorescence detector

Luminescence ◽  
2017 ◽  
Vol 32 (7) ◽  
pp. 1145-1149 ◽  
Author(s):  
Elif Özdemir ◽  
Sevgi Tatar Ulu
Keyword(s):  
Human Plasma ◽  
Hplc Method ◽  
Dansyl Chloride ◽  
Fluorescence Detector ◽  
Human Plasma And Urine ◽  
Highly Sensitive

Rapid and validated fluorometric HPLC method for determination of gabapentin in human plasma and urine for clinical application

2011 ◽  
Vol 37 (1) ◽  
pp. 89-94 ◽  
Author(s):  
T. Yagi ◽  
T. Naito ◽  
Y. Mino ◽  
Y. Takashina ◽  
K. Umemura ◽  
...  
Keyword(s):  
Human Plasma ◽  
Clinical Application ◽  
Hplc Method ◽  
Human Plasma And Urine

Determination of Methoxamine in Human Plasma and Urine by a Validated HPLC-Fluorescence Detection Method

2013 ◽  
Vol 96 (5) ◽  
pp. 987-990
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Detection Method ◽  
Hplc Method ◽  
Fluorescent Product ◽  
Hplc Separation ◽  
Human Plasma And Urine ◽  
System Suitability ◽  
Isocratic Hplc

Abstract A sensitive HPLC method for the determination of methoxamine in human plasma and urine is described. The method is based on derivatization of methoxamine with 4-chloro-7-nitrobenzofurazan in borate buffer of pH 9.5 to yield a yellow, fluorescent product. Isocratic HPLC separation was achieved on an Inertsil C18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase methanol–water (60 + 40, v/v) at a flow rate of 1.2 mL/min. Fluorescence detection was used at excitation and emission wavelengths of 458 and 521 nm, respectively. The assay was linear over the concentration ranges of 10–250 and 20–300 ng/mL for plasma and urine, respectively. The LOD values were 3.3 and 6.8 ng/mL and the LOQ values were 10 and 20 ng/mL for plasma and urine, respectively. The extraction recoveries were more than 97.10%. After strict validation, the method indicated good performance in terms of linearity, sensitivity, precision, accuracy (recovery), robustness, and system suitability, and it was successfully applied to the determination of methoxamine in human plasma and urine.

scholarly journals Highly sensitive determination of PLP in human plasma with HPLC method.

1990 ◽  
Vol 36 (6) ◽  
pp. 521-529 ◽  
Author(s):  
Naoyuki HIROSE ◽  
Nobuko KUBO ◽  
Haruhito TSUGE
Keyword(s):  
Human Plasma ◽  
Hplc Method ◽  
Highly Sensitive ◽  
Sensitive Determination

Extractionless HPLC Method for the Determination of Naproxen in Human Plasma and Urine

1993 ◽  
Vol 26 (11) ◽  
pp. 2341-2348 ◽  
Author(s):  
Th. Karidas ◽  
A. Avgerinos ◽  
S. Malamataris
Keyword(s):  
Human Plasma ◽  
Hplc Method ◽  
Human Plasma And Urine
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